Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Waters Unveils Industry’s First Integrated LC/MS Platform at WCBP 2013

Published: Tuesday, January 29, 2013
Last Updated: Monday, January 28, 2013
Bookmark and Share
Complementing the expanded biopharmaceutical platform solution are new charged surface hybrid particle column and glycoworks kit for glycan preparation.

Waters Corporation has reinforced its commitment to advancing biotherapeutic characterization technology at the Well Characterized Biotechnology Pharmaceuticals 2013 Symposium (WCBP 2013).

More specifically, Waters® has introduced an expansion of its Biopharmaceutical Platform Solution with UNIFI®, new ACQUITY UltraPerformance LC® (UPLC®)CSH130 C18 and XSelect™ HPLC CSH130 C18 columns for peptide mapping, and three GlycoWorks™ kits for glycan labeling and sample preparation.

These innovations represent Waters’ continued focus on developing targeted solutions for developers of biotherapeutics and biosimilars, as well as related contract laboratory organizations.

These new products further progress routine biotherapeutic analysis, particularly for glycoproteins that require analysis of glycan modifications in addition to detailed protein and peptide level structural analysis.

Armed with more rapid and accurate glycosylation knowledge throughout the development and manufacturing process, biopharmaceutical companies can gain a greater degree of control over the critical quality attributes of their molecules.

Such capabilities are in-line with increasing regulatory expectations aimed at guaranteeing safe and efficacious biotherapeutics.

Waters Biopharmaceutical Platform Solution with UNIFI
The Biopharmaceutical Platform Solution brings together UPLC/MS characterization technology with the UNIFI Scientific Information System that was first developed for intact protein mass analysis, peptide mapping and supporting general bio-separations.

Today, Waters extended the solution’s reach to support a mix of quadrupole time-of-flight (Q-Tof) MS and optical detection instruments within a networked laboratory workgroup.

The ability to deploy UNIFI-based systems within regulated or non-regulated laboratory environments provides biopharmaceutical companies the flexibility to employ high resolution UPLC bioseparations and high performance mass analysis across the entirety of their development and quality organizations.

The newly introduced released Glycan application workflow expands the platform’s capabilities to support routine assignment and profiling of released glycans using fluorescence detection.

The combination of high performance UPLC HILIC (hydrophilic interaction chromatography) separations, Waters’ calibration standards and reagents, and access to the NIBRT/Waters GlycoBase 3+ UPLC glycan unit reference database enables confident glycan assignments, quantification, and profiling.

Developed by Professor Pauline Rudd's research team at Ireland's National Institute for Bioprocessing Research and Training (NIBRT), the GlycoBase 3+ Database is the first-of-its-kind repository of glycan chromatographic retention data, expressed in glucose calibration units, and encompassing the diverse sets of glycan structures associated with modern biotherapeutic glycoproteins.

The Biopharmaceutical Platform Solution with UNIFI now features:

• ACQUITY UPLC H-Class and the H-Class Bio System featuring a bio-inert flow path and quaternary solvent management with Auto-Blend Plus™ Technology, for flexibility in executing high-resolution bioseparations;
• Waters Peptide, Protein, and Glycan Separation Technology Columns, engineered with the selectivity to leverage the characteristics of biomolecules and QC-tested to ensure consistent results;
• Waters Analytical Standards and Reagents for biopharmaceutical analysis, enabling SEC (size exclusion chromatography) and released glycan separation calibration, and system check standards for intact mass, peptide map, and released glycan workflows;
• Xevo® G2-S Q-Tof Mass Spectrometer, a highly sensitive exact-mass quantitative and qualitative benchtop MS system featuring Waters’ proprietary StepWave™ ion optics technology, a unique, off-axis ion source technology that delivers robust, reproducible, class-leading sensitivity to mass spectrometry;
• UNIFI Scientific Information System, an interactive, workflow-driven data platform for flexible instrument control, advanced data processing, and comprehensive reporting, with GxP laboratory compatibility that enables routine deployment in workstation or workgroup laboratory configurations;
• GlycoBase 3+ database, a first-of-its-kind library of chromatographic retention positions expressed in glucose units for sets of glycan structures associated with a range of biotherapeutics.

Waters Charged Surface Hybrid Particle Technology Columns
Waters’ new CSH130 particle technology columns offer unique selectivities for UPLC and HPLC peptide mapping and proteomics applications. The ACQUITY UPLC CSH130 C18 and XSelect™ HPLC CSH130 C18 Columns set a new standard in the analysis and purification of peptides for the quality of information generated by UPLC and UPLC/MS assays. The columns are available in a variety of particle sizes and column dimensions.

The novel columns feature Waters’ synthesis process for its Charged Surface Hybrid particles, which imparts a low level positive charge to the surface of each particle. This particle technology allows the columns to be used with a weaker acid modifier such as formic acid to obtain greater resolving power and improved method sensitivity compared to standard LC/MS methods that specify the use of MS signal-suppressing ion pairing additives such as trifluoroacetic acid (TFA).

The benefits of the Waters Biopharmaceutical Platform Solution with UNIFI for glycan analysis, as well as for biosimilar comparison and peptide mapping, are being presented in several posters being given at the WCBP 2013.

GlycoWorks Line of Consumable Products
Waters’ new GlycoWorks Line of Consumable Products brings together everything needed for the preparation of glycans for analysis, including sample preparation, labeling, clean-up and standards, along with methodologies and troubleshooting tools.

The product line includes two GlycoWorks prep kits, one for high throughput and another for single use, and a labeling kit. Each prep kit includes enzymatic deglycosylation with a variety of enzyme choices, HILIC sorbent for the cleanup and capture of released glycans, and a control standard to help with method validation, development and troubleshooting. A GlycoWorks 2-AB labeling kit includes four reaction reagents for use with released glycans.

Other consumable products from Waters that support glycan analysis include ACQUITY UPLC BEH Glycan columns that have been specifically developed and QC tested to provide superior UPLC component resolution for glycan structures; a Dextran calibration ladder that is 2-AB labeled for calibration; and a Glycan performance standard, a control standard of 2-AB labeled Human IgG.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More Than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Waters’ Centers of Innovation Program Honors Ohio State University Laboratory
Wysocki-led facility recognized for proteomics, disease research and mass spectrometry excellence.
Wednesday, September 23, 2015
Waters Corporation Announces Christopher J. O'Connell as CEO
Chris joins Waters® from Medtronic plc.
Thursday, July 02, 2015
Waters Centers of Innovation Program Honors CSU Laboratory
Proteomics and metabolomics facility recognized for innovation in biological mass spectrometry.
Wednesday, October 29, 2014
Waters Q3 Revenues up 8 Percent
Company reports third quarter sales of $493 million, an increase of 8% versus sales of $457 million in the third quarter of 2013.
Tuesday, October 21, 2014
Waters, Chinese Pharmacopeia Commission Establish Joint Open Laboratory
Located in the laboratory building of Beijing Zhendong Guangming Drug Research Institute, the new facility is over 400m2.
Thursday, July 10, 2014
New Solutions to Old Challenges
Waters Corporation introduces a new NuGenesis Lab Management System (LMS) that integrates sample management functions and business operations across the enterprise. Technology Networks' Editor Helen Gillespie interviewed Waters' Garrett Mullen for details.
Tuesday, July 01, 2014
Waters, Prosolia Exclusive Agreement for DESI Technology for Clinical MS Applications
Prosolia DESI Technology now available on SYNAPT G2 Si and Xevo G2-XS QTof mass spectrometers.
Monday, June 16, 2014
Waters, Omics LLC Partner to Advance Petroleum Sample Analysis
Collaboration Combines the Value of Ion Mobility Mass Spectrometry (IM-MS) and Accurate Mass Information with Petroleomics Data Processing.
Monday, June 16, 2014
Waters ACQUITY UPLC TQD System Receives Chinese FDA Approval
Chinese FDA approves ACQUITY UPLC TQD system for routine diagnostic applications and neonatal metabolites diseases screening.
Thursday, June 05, 2014
Waters ACQUITY QDa Detector Takes Pittcon Editors' Award
Product receives Editors' Silver award for the most innovative product introduced at Pittcon 2014.
Monday, March 10, 2014
Waters Report 8% Revenue Increase
Fourth quarter 2013 sales were $565 million, an increase of 8% compared to sales of $522 million in the fourth quarter of 2012.
Tuesday, January 28, 2014
Waters Teams With Biochemical Society on Proteomics Training Day and Symposium
The consecutive events will take place on March 24 - 27, 2014 in Chester, England.
Wednesday, November 27, 2013
Waters Acquires Nonlinear Dynamics
Nonlinear Dynamics is best known for their Progenesis software which offers researchers unique ways to analyze and visualize the raw proteomic data.
Thursday, August 08, 2013
Waters Sponsors Clinical Chemistry Fellowship
The two-year, postdoctoral fellowship at the University of California, San Diego School of Medicine will emphasize a combination of training in classical clinical chemistry and specialty areas.
Wednesday, July 31, 2013
New Phenome Centre will Decipher Roles of Nature and Nurture in Human Health
The MRC-NIHR Phenome Centre will examine around one hundred thousand blood and urine samples every year, putting the UK at the forefront of a revolution in health and medical research.
Wednesday, June 05, 2013
Scientific News
Fixing Holes in the Heart Without Invasive Surgery
UV-light enabled catheter is a medical device which represents a major shift in how cardiac defects are repaired.
Chromosomal Chaos
Penn study forms basis for future precision medicine approaches for Sezary syndrome
Enzyme Malfunction May be Why Binge Drinking Can Lead to Alcoholism
A new study in mice shows that restoring the synthesis of a key brain chemical tied to inhibiting addictive behavior may help prevent alcohol cravings following binge drinking.
Key to Natural Detoxifier’s Reactivity Discovered
Results have implications for health, drug design and chemical synthesis.
New Treatment for Obesity Developed
Researchers at the University of Liverpool, working with a global healthcare company, have helped develop a new treatment for obesity.
New Protein Found in Immune Cells
Immunobiologists from the University of Freiburg discover Kidins220/ARMS in B cells and demonstrate its functions.
Will Brain Palpation Soon Be Possible?
Researchers have developed non-invasive brain imaging technique which provides the same information as physical palpation.
Shaking Up the Foundations of Epigenetics
Researchers at the Centre for Genomic Regulation (CRG) and the University of Barcelona (UB) published a study that challenges some of the current beliefs about epigenetics.
Groundbreaking Computer Program Diagnoses Cancer in Two Days
Researchers have combined genetics with computer science and created a new diagnostic technology can with 85 per cent certainty identify the source of the disease and thus target treatment and, ultimately, improve the prognosis for the patient.
Michigan Researchers Use Raman Spectroscopy
inVia confocal Raman microscope used in the study of various childhood diseases.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos