Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Sigma Life Science to Distribute Olink Bioscience's Protein-Protein Interaction Assay

Published: Tuesday, February 12, 2013
Last Updated: Tuesday, February 12, 2013
Bookmark and Share
The products complement Sigma's antibody, RNAi and gene editing technologies.

Sigma-Aldrich® Corporation announced that Sigma® Life Science have signed an agreement to distribute worldwide Olink Bioscience's Duolink® In Situ products, which present a new disruptive method for imaging and measuring protein-protein interactions in unmodified cells. The Duolink In Situ products work in tandem with Sigma Life Science's broad antibody portfolio and will allow researchers to target the precise protein of interest and enable robust analyses and imaging. 

The versatile technology, among other applications, can be used in combination with CompoZr® Zinc Finger Nuclease-mediated gene editing to track knocked-out or knocked-in genes and with MISSION® RNA interference technologies to track protein expression following gene knockdown. "The synergy between Olink and Sigma Life Science's global support network will help the scientific community to access and to accelerate the adoption of the powerful Duolink technology," said Josef Zihlmann, Vice President at Sigma Life Science.

The proprietary PLA® technology allows Olink's Duolink In Situ products to quickly image the locations of single protein-protein interactions regardless of strength, measure protein expression levels, and identify relative changes in post-translational modification events. The technique relies upon two target-specific primary antibodies, a "plus" and "minus" pair of species-specific PLA probes conjugated to DNA oligos that—when a target pair of proteins are in close proximity—can hybridize to trigger a rolling circle amplification reaction. Fluorescent detection probes bind the several hundred-fold amplified DNA at high density, allowing visualization of single protein—protein interactions inside an intact cell using a standard fluorescence microscope.

"The ability to visualize these protein-protein interactions in unmodified cells under endogenous expression and at very low levels is a fundamental advance that allows greater insight into basic biology, pathways, spatial phenomena and potential therapeutic targets in those pathways," said Zihlmann.

More than 350 publications in the past six years have relied upon the Duolink method, which is featured in an average of five publications per week by academic and pharmaceutical researchers. The Sigma Life Science Prestige® antibody collection complements the technology with its portfolio of more than 14,000 antibodies that have been validated by immunohistochemistry, a key requirement for Duolink In Situ products to target specific proteins of interest.

"We believe the agreement with Sigma Life Science will build awareness and develop the market for Duolink In Situ through their global sales and support organization serving our growing customer base locally," said Simon Fredriksson, CEO and President of Olink Bioscience. "Sigma Life Science's extensive product range for cell biology is a perfect context for customers interested in Duolink In Situ."

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Sigma-Aldrich Announces Agreement on Assets Sale to Honeywell
Asset sale includes Sigma-Aldrich-branded solvents and inorganics business in the EEA.
Tuesday, October 20, 2015
Sigma-Aldrich Stockholders Approve Merck Acquisition
The transaction, expected to close mid-2015, remains subject to the satisfaction of the closing conditions set forth in the merger agreement, including regulatory approvals.
Monday, December 08, 2014
SAFC Expands Continuous Flow Capabilities
Sigma-Aldrich custom manufacturing services business unit, SAFC® Commercial, has installed a new commercial-scale continuous flow reactor at its Sheboygan, WI facility.
Wednesday, May 21, 2014
Sigma-Aldrich® Partners with Scripps Research Institute
The agreement marks first partnership of its kind between a research institution and reagents company.
Friday, July 19, 2013
Sigma-Aldrich Completes Acquisition of BioReliance
Sigma-Aldrich Corporation completes its acquisition of BioReliance Holdings, Inc. for $350 million in cash.
Wednesday, February 01, 2012
Sigma Life Science Collaborates with Cofactor Genomics to Develop Publically Available Rat Genome Database
Sigma Life Science announces that its SAGE Labs initiative has partnered with Cofactor Genomics in an effort to sequence genomes for six of the most widely used strains of rat. Cofactor Genomics plans to generate and analyze sequence data using next generation sequencing systems, while SAGE Labs plans to provide the samples and to host a new, free public database at in late 2011.
Thursday, February 03, 2011
Sigma® Life Science Awarded Silver in Top Ten Innovations 2010
Company’s CompoZr® cell lines ranked second as The Scientist magazine unveils this year’s best life science innovations.
Monday, December 06, 2010
Plasticell Signs Technology Agreement with Sigma-Aldrich
Collaboration to produce novel turnkey solution for stem cell research.
Tuesday, November 30, 2010
Sigma® Life Science Enters Genetically Modified Cell Market
Sigma Life Science launches range of engineered cell lines exploiting CompoZr® zinc finger nuclease technology.
Monday, September 06, 2010
Sigma® Life Science and Switchgear Genomics to Co-Develop Simplified Novel MicroRNA Target Validation System
SwitchGear Genomics and Sigma’s lentiviral technology combined to simplify miRNA target validation, enabling researchers to investigate gene regulation.
Tuesday, August 10, 2010
Sigma-Aldrich Licenses from NeuroSurvival Technologies a Marker for Molecular Imaging of Apoptosis in Vivo
The marker, Apo-TRACE™, offers a breakthrough in advancing apoptosis-related research in a range of fields in biology and medicine.
Wednesday, February 27, 2008
Sigma-Aldrich Grants Pfizer Non-Exclusive Worldwide License to Use ddRNAi in Research Programs
The license enables Pfizer to undertake research activities throughout its global operations using ddRNAi technology.
Thursday, January 04, 2007
Sigma-Proligo Expands into Genopole® d'Evry Biopark
Sigma-Proligo provides researchers with high-quality oligonucleotides due to a high throughput synthesis platform.
Friday, October 20, 2006
Sigma-Aldrich Announces Service Provider for WGA Technology
Sigma announces MOgene as its first service provider of the GenomePlex® Whole Genome Amplification technology.
Wednesday, May 31, 2006
Sigma-Aldrich Sponsors Full-Text Article on High Content Viral Library Screens
The authors reported the creation of a lentiviral shRNA library to knock down genes for genome-wide screening.
Thursday, April 27, 2006
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos