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Crystal Structures of a Glycoside Hydrolase Family 20 Lacto-N-Biosidase from Bifidobacterium bifidum

Published: Monday, March 18, 2013
Last Updated: Monday, March 18, 2013
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Scientists have determined the first three-dimensional structures of lacto-N-biosidase from Bifidobacterium bifidum JCM1254 in complex with LNB and LNB-thiazoline (Gal-1,3-GlcNAc-thiazoline) at 1.8 Å resolution.

Background: Infant gut-associated bifidobacteria possess lacto-N-biosidase, which releases lacto-N-biose I (LNB) from human milk oligosaccharides.

Results: The crystal structures of lacto-N-biosidase complexed with LNB and LNB-thiazoline were determined.

Conclusion: The intermediate analogue complex allows the proposal of a conformational reaction coordinate.

Significance: The structures of a key enzyme in the colonization of human commensal bacteria provided its structural basis and insight into the development of inhibitors.

This article was published online in The Journal of Biological Chemistry and is free to access.

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