Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Wyatt Calypso Succeeds in ABRF-MIRG Study

Published: Monday, April 29, 2013
Last Updated: Tuesday, April 09, 2013
Bookmark and Share
Wyatt Technology reported the success of the Calypso composition-gradient multi-angle light scattering (CG-MALS) system in a study conducted by the Association of Biomolecular Research Facilities (ABRF).

The conclusions of the 2012 MIRG study were announced at the ABRF 2013 annual meeting in Palm Springs earlier this month.

In the study, a pair of unknown proteins, prepared and characterized by the ABRF, was sent out to multiple labs to determine affinity and stoichiometry of binding. In a single afternoon consisting of 2 CG-MALS runs, the Wyatt Calypso system correctly determined that one molecule of Protein Y (26.3 kDa) binds 2 molecules of Protein X (11.9 kDa), one with KD of 10 nM and the other with 14 µM. This analysis agreed perfectly with the extensive AUC and ITC measurements performed by ABRF prior to distributing the samples.

The CG-MALS measurement was the only solution-based measurement among the participating labs. The others used surface plasmon resonance (SPR) instruments which require immobilization of one of the binding partners. Only 4 of 13 SPR labs correctly measured and interpreted the data, typically taking 1-2 days even though the chip immobilization recipe and regeneration were provided by the organizers.

Interestingly, the affinities determined by SPR were significantly weaker than those found by any of the solution-based measurements (AUC, ITC, CG-MALS), indicating that, in this instance, immobilization modifies the interaction.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

ILSC to Highlight Developments in Applications and Characterization of Nanoparticles
For the first time, this year’s ILSC will be open to all rather than exclusively to Wyatt’s customers.
Thursday, August 21, 2014
Determining Charge and Diffusion Interaction Parameter
New study from Wyatt Technology showcases developments in the ability to characterize colloidal stability to establish suitability of protein formulations for drug therapeutics.
Tuesday, June 03, 2014
New Whitepaper from Wyatt on Branching in Synthetic and Natural Polymers
New whitepaper highlights the importance of multi-angle light scattering instrumentation.
Tuesday, February 11, 2014
Wyatt Technology Recognized Among Top 15 Advances

Thursday, December 19, 2013
Key Protein Interactions Show Promise for Difficult Biomolecular Systems
Wyatt Technology publishes a new application note highlighting a study on the complex interactions between proteins and how they modulate the rotational direction of bacteria flagella.
Tuesday, December 10, 2013
Using Appropriate Buffer Conditions Improves Sample Preparation for Structural Analysis
New Customer Application Note Available from Wyatt Technology.
Monday, July 29, 2013
Wyatt Technology Ranked Among The Scientist Magazine’s Best Places to Work in Industry
Wyatt was named #5 on the worldwide list of companies this year.
Tuesday, July 02, 2013
Wyatt Technology Celebrates 30th Years
Company will toast 300 years of macromolecular characterization expertise at the 2012 International Light Scattering Colloquium.
Thursday, July 12, 2012
Wyatt Technology Ranks as #4 in The Scientist’s 10th Annual Best Places to Work Survey
Company ranked fourth place due to their ability to nurture scientific excellence and provide high staff satisfaction.
Monday, June 04, 2012
MALS Detectors Used in Breakthrough Virus Research
Wyatt Technology Corporation have announced that its instruments were used in a recent landmark study that transforms the previous scientific understanding of immunity to viral diseases like the common cold, 'winter vomiting' and gastroenteritis.
Wednesday, January 05, 2011
Wyatt Technology Instrumentation Used Against Bacteriophage Infections in Dairy Production
Wyatt’s instruments facilitate the study of protein-protein complexes, identifying their potential to serve as inhibitors of bacteriophage infections.
Wednesday, November 17, 2010
Wyatt Technology to Host 21st Annual International Light Scattering Colloquium
The annual event will showcase Wyatt’s new Möbiu? mobility instrument.
Thursday, August 19, 2010
Wyatt Technology will Host 21st Annual International Light Scattering Colloquium
The colloquium will welcome an array of Wyatt Technology light scattering customers to showcase company's new Möbiu? mobility instrument.
Friday, May 28, 2010
University of Navarra Implements Wyatt Dynamic Light Scattering Instrument
The DynaPro instrument will be utilized to characterize phase transitions and structures in micellar polymeric systems.
Thursday, April 29, 2010
Wyatt Technology Uses Composition Gradient Multi-Angle Light Scattering for Characterizing Protein Inhibition in Solution Without Labeling or Immobilization
Wyatt Technology Corporation, announces the availability of a new application note demonstrating the advanced capability of Composition Gradient Multi-angle Light Scattering (CG-MALS) technology.
Wednesday, September 23, 2009
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Self-Assembling, Biomimetic Membranes May Aid Water Filtration
A synthetic membrane that self assembles and is easily produced may lead to better gas separation, water purification, drug delivery and DNA recognition, according to an international team of researchers.
Researchers Discover Immune System’s 'Trojan Horse'
Oxford University researchers have found that human cells use viruses as Trojan horses, transporting a messenger that encourages the immune system to fight the very virus that carries it.
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
How Cholesterol Leads to Clogged Arteries
A new study shows that when immune cells called neutrophils are exposed to cholesterol crystals, they release large extracellular web-like structures that trigger the production of inflammatory molecules linked to artherosclerosis.
Genetic Tug of War
Researchers have reported on a version of genetic parental control in mice that is more targeted, and subtle than canonical imprinting.
Ultrafast DNA Diagnostics
New technology developed by UC Berkeley bioengineers promises to make a workhorse lab tool cheaper, more portable and many times faster by accelerating the heating and cooling of genetic samples with the switch of a light.
Researchers Discover New Type of Mycovirus
Virus infects the fungus Aspergillus fumigatus, which can cause the human disease aspergillosis.
Error Correction Mechanism in Cell Division
Cell biologists have reported an advance in understanding the workings of an error correction mechanism that helps cells detect and correct mistakes in cell division early enough to prevent chromosome mis-segregation and aneuploidy, that is, having too many or too few chromosomes.
How to Become a Follicular T Helper Cell
Uncovering the signals that govern the fate of T helper cells is a big step toward improved vaccine design.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!