Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Wyatt Calypso Succeeds in ABRF-MIRG Study

Published: Monday, April 29, 2013
Last Updated: Tuesday, April 09, 2013
Bookmark and Share
Wyatt Technology reported the success of the Calypso composition-gradient multi-angle light scattering (CG-MALS) system in a study conducted by the Association of Biomolecular Research Facilities (ABRF).

The conclusions of the 2012 MIRG study were announced at the ABRF 2013 annual meeting in Palm Springs earlier this month.

In the study, a pair of unknown proteins, prepared and characterized by the ABRF, was sent out to multiple labs to determine affinity and stoichiometry of binding. In a single afternoon consisting of 2 CG-MALS runs, the Wyatt Calypso system correctly determined that one molecule of Protein Y (26.3 kDa) binds 2 molecules of Protein X (11.9 kDa), one with KD of 10 nM and the other with 14 µM. This analysis agreed perfectly with the extensive AUC and ITC measurements performed by ABRF prior to distributing the samples.

The CG-MALS measurement was the only solution-based measurement among the participating labs. The others used surface plasmon resonance (SPR) instruments which require immobilization of one of the binding partners. Only 4 of 13 SPR labs correctly measured and interpreted the data, typically taking 1-2 days even though the chip immobilization recipe and regeneration were provided by the organizers.

Interestingly, the affinities determined by SPR were significantly weaker than those found by any of the solution-based measurements (AUC, ITC, CG-MALS), indicating that, in this instance, immobilization modifies the interaction.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

ILSC to Highlight Developments in Applications and Characterization of Nanoparticles
For the first time, this year’s ILSC will be open to all rather than exclusively to Wyatt’s customers.
Thursday, August 21, 2014
Determining Charge and Diffusion Interaction Parameter
New study from Wyatt Technology showcases developments in the ability to characterize colloidal stability to establish suitability of protein formulations for drug therapeutics.
Tuesday, June 03, 2014
New Whitepaper from Wyatt on Branching in Synthetic and Natural Polymers
New whitepaper highlights the importance of multi-angle light scattering instrumentation.
Tuesday, February 11, 2014
Wyatt Technology Recognized Among Top 15 Advances

Thursday, December 19, 2013
Key Protein Interactions Show Promise for Difficult Biomolecular Systems
Wyatt Technology publishes a new application note highlighting a study on the complex interactions between proteins and how they modulate the rotational direction of bacteria flagella.
Tuesday, December 10, 2013
Using Appropriate Buffer Conditions Improves Sample Preparation for Structural Analysis
New Customer Application Note Available from Wyatt Technology.
Monday, July 29, 2013
Wyatt Technology Ranked Among The Scientist Magazine’s Best Places to Work in Industry
Wyatt was named #5 on the worldwide list of companies this year.
Tuesday, July 02, 2013
Wyatt Technology Celebrates 30th Years
Company will toast 300 years of macromolecular characterization expertise at the 2012 International Light Scattering Colloquium.
Thursday, July 12, 2012
Wyatt Technology Ranks as #4 in The Scientist’s 10th Annual Best Places to Work Survey
Company ranked fourth place due to their ability to nurture scientific excellence and provide high staff satisfaction.
Monday, June 04, 2012
MALS Detectors Used in Breakthrough Virus Research
Wyatt Technology Corporation have announced that its instruments were used in a recent landmark study that transforms the previous scientific understanding of immunity to viral diseases like the common cold, 'winter vomiting' and gastroenteritis.
Wednesday, January 05, 2011
Wyatt Technology Instrumentation Used Against Bacteriophage Infections in Dairy Production
Wyatt’s instruments facilitate the study of protein-protein complexes, identifying their potential to serve as inhibitors of bacteriophage infections.
Wednesday, November 17, 2010
Wyatt Technology to Host 21st Annual International Light Scattering Colloquium
The annual event will showcase Wyatt’s new Möbiu? mobility instrument.
Thursday, August 19, 2010
Wyatt Technology will Host 21st Annual International Light Scattering Colloquium
The colloquium will welcome an array of Wyatt Technology light scattering customers to showcase company's new Möbiu? mobility instrument.
Friday, May 28, 2010
University of Navarra Implements Wyatt Dynamic Light Scattering Instrument
The DynaPro instrument will be utilized to characterize phase transitions and structures in micellar polymeric systems.
Thursday, April 29, 2010
Wyatt Technology Uses Composition Gradient Multi-Angle Light Scattering for Characterizing Protein Inhibition in Solution Without Labeling or Immobilization
Wyatt Technology Corporation, announces the availability of a new application note demonstrating the advanced capability of Composition Gradient Multi-angle Light Scattering (CG-MALS) technology.
Wednesday, September 23, 2009
Scientific News
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Dengue Virus Exposure May Amplify Zika Infection
Researchers at Imperial College London have found that the previous exposure to the dengue virus may increase the potency of Zika infection.
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Identifying Novel Types of Forensic Markers in Degraded DNA
Scientists have tried to verify the nucleosome protection hypothesis by discovering STRs within nucleosome core regions, using whole genome sequencing.
Proteins in Blood of Heart Disease Patients May Predict Adverse Events
Nine-protein test shown superior to conventional assessments of risk.
Higher Frequency of Huntington's Disease Mutations Discovered
University of Aberdeen study shows that the gene change that causes Huntington's disease is much more common than previously thought.
Starving Stem Cells May Enable Scientists To Build Better Blood Vessels
Researchers from the University of Illinois at Chicago College of Medicine have uncovered how changes in metabolism of human embryonic stem cells help coax them to mature into specific cell types — and may improve their function in engineered organs or tissues.
Rates of Nonmedical Prescription Opioid Use Disorder Double in 10 Years
Researchers at NIH have found that the nonmedical use of prescription opioids has more than doubled among adults in the United States from 2001-2002 to 2012-2013.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!