Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Device Finds Stray Cancer Cells in Patients’ Blood

Published: Wednesday, April 10, 2013
Last Updated: Wednesday, April 10, 2013
Bookmark and Share
A microfluidic device that captures circulating tumor cells could give doctors a noninvasive way to diagnose and track cancers.

Doctors typically diagnose cancer via a biopsy, which can be invasive and expensive. A better way to diagnose the disease would be to detect telltale tumor cells floating in the bloodstream, but such a test has proved difficult to develop because stray cancer cells are rare, and it’s difficult to separate them from the mélange of cells in circulation.

Now researchers from Massachusetts General Hospital and Harvard Medical School say they’ve built a microfluidic device that can quickly grab nearly any type of tumor cell, an advance that may one day lead to simple blood tests for detecting or tracking cancer.

Similar, existing devices—including earlier versions developed by the authors of the study in Wednesday’s online issue of Science Translational Medicine—depend on tumor-specific biomarkers on the surface of the cells to pull them out of a blood sample, meaning that a given device won’t work for all cancer types. What’s more, the efficiency by which the tumor cells are purified from other cell types is generally low and time-consuming. In a given blood sample, circulating tumor cells are rare—there may be only one tumor cell for every billion cells.

The new device is a “substantial step forward from previous microfluidic devices,” says Peter Kuhn, a circulating-tumor-cell researcher at the Scripps Research Institute. Kuhn was not involved in the study. The device combines existing microfluidic techniques of cell sorting into a single device, he says. The result is that the tumor cells can be pulled out of a blood sample quicker, and without prior knowledge of their molecular characteristics.

Mehmet Toner, director of the BioMicroElectroMechanical Systems Resource Center at MGH, and colleagues report that their latest chip can isolate circulating-tumor cells in the blood, and could apply to all types of cancer. “For our earlier chip, you needed to know something on the surface of the tumor cells,” says Toner. In those devices, a small sample of blood would flow through microfluidic chambers, some of which contained an antibody that grabbed tumor cells. That system also took four to five hours to process a single blood sample. “But for early detection and to make this useful for virtually all cancers, we needed to increase the throughput and to make it [tumor-type] independent,” he says.

Identifying these wandering tumor cells could also help researchers study a cancer’s progression and help doctors track treatments or screen for new cases. By studying the surface proteins or genetic profiles of the cancer cells, doctors and researchers could learn which mutations are present in the cancer and perhaps tailor molecularly targeted treatments accordingly. The authors show that 15 tumor cells were recovered from a blood sample from a prostate cancer patient. The gene expression levels of each cell were studied individually and a mix of mutations was found.

The device developed by Toner’s group combines magnetic labeling of cells and microfluidic sorting to process a sample of blood in about an hour or two. To capture tumor cells regardless of their cancer type, the system first tags white blood cells with magnetic beads that are covered with antibodies that recognize proteins on the surface of the immune cells. The sample is then passed into microfluidic chambers that clear out red blood cells, plasma, and unused free magnetic beads based on their size. Then the device discards the tagged white blood cells using a magnetic field. “In the past, we were focused on tumor cells that we know very little about,” says Toner. “Here, we throw away the cells we know everything about, the blood cells,” he says.

The advantage of the new cell-sorting device over previous attempts is that it successfully brings together multiple technologies, such as size separation and magnetic-tag separation, already used in the field, says Gajus Worthington, president and CEO of Fluidigm, a California company that produces microfluidic devices for biomedical research. “The key thing here is the integration, which is crucial to anything related to single-cell work,” he says. All the steps in Toner’s device take place in similar volumes. “If you have to go from one microstep back to macrostep back to microstep, there are losses and complexity, which leads to noise,” says Worthington.

Toner notes that the Holy Grail for circulating-tumor-cell technology would be to diagnose patients early. “About 10 percent of cancer patients survive if they are diagnosed late, but almost 90 percent survive if they are diagnosed early,” says Toner. But whether or not these circulating tumor cells can be found in early-stage patients is not yet clear, says Luis Diaz, an oncologist at Johns Hopkins University School of Medicine. Diaz was not involved in the study. “Early-stage cancers might release very few cells into circulation,” he says. “That’s historically the problem with circulating tumor cells; you can only find them in advanced cancers.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 4,000+ scientific posters on ePosters
  • More Than 5,300+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Unique Visual Stimulation May Be New Treatment for Alzheimer’s
Noninvasive technique reduces beta amyloid plaques in mouse models of Alzheimer’s disease.
Thursday, December 08, 2016
How the Brain Recognizes Faces
Machine-learning system spontaneously reproduces aspects of human neurology.
Wednesday, December 07, 2016
Radiation-Free Imaging in the Brain
Scientists create sensors that use proteins to detect particular targets through induced blood flow changes.
Monday, December 05, 2016
New Method for Analyzing Crystal Structure
Exotic materials called photonic crystals reveal their internal characteristics with new method.
Monday, November 28, 2016
Biomarker Guiding Cancer Therapy
Biologists link levels of Mena protein to breast cancer cells’ sensitivity to chemotherapy.
Tuesday, November 22, 2016
Capsule Achieves Long-Term Drug Delivery
Novel drug delivery method could aid in elimination of malaria and treatment of many other diseases.
Monday, November 21, 2016
Synthetic Cells Isolate Genetic Circuits
Encapsulating molecular components in artificial membranes offers more flexibility in designing circuits.
Tuesday, November 15, 2016
Turning Greenhouse Gas into Gasoline
New catalyst provides design principles for producing fuels from carbon dioxide emissions.
Tuesday, November 15, 2016
New Approach Against Salmonella
Researchers have developed a strategy to immunize against microbes that invade the gastrointestinal tract, including Salmonella.
Tuesday, November 08, 2016
Laser Particles Could Provide Sharper Tissue Images
New imaging technique stimulates particles to emit laser light, could create higher-resolution images.
Tuesday, November 08, 2016
Engineers Design New Weapon Against Bacteria
Researchers have successfully engineered antimicrobial peptides that can kill bacterial strains resistant to existing antibiotics.
Thursday, November 03, 2016
Predicting Cancer Cells’ Response to Chemotherapy
Researcher develop method for testing cell ability to perform different types of DNA repair, which can reveal tumors’ sensitivity to drugs.
Wednesday, November 02, 2016
Nanobionic Spinach Detects Dangerous Chemicals
Scientists have changed spinach plants into biosensors that can detect harful chemicals and wirelessly relay the information.
Tuesday, November 01, 2016
Fighting Cancer with the Power of Immunity
Researchers at MIT have used a combination of four different therapies to activate both of the immune system’s two branches, producing a coordinated attack that led to the complete disappearance of large, aggressive tumors in mice.
Friday, October 28, 2016
Fighting Cancer with Immune Response
New treatment elicits two-pronged immune response that destroys tumors in mice.
Tuesday, October 25, 2016
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Personality Traits, Psychiatric Disorders Linked to Specific Genomic Locations
Researchers have unearthed genetic correlations between personality traits and psychiatric disorders.
Forensic 3D Documentation of Skin Injuries
In this study, the validity of using photogrammetry for documenting injuries in a pathological context was demonstrated.
3-D Printed Dog’s Nose Improves Vapor Detection
By mimicking how dogs get their whiffs, a team of government and university researchers have demonstrated that “active sniffing” can improve by more than 10 times the performance of current technologies that rely on continuous suction to detect trace amounts of explosives and other contraband.
New Markers for Forensic Body-fluid Identification
University of Bonn researchers have successfully identified specific Micro-RNA signatures to help forensically identify body fluids.
Genetics Control Regenerative Properties Of Stem Cells
Researchers define how genetic factors control regenerative properties of blood-forming stem cells.
Major Neuroscience Initiative Launched
Tianqiao and Chrissy Chen Institute invest $115 million to further expand neuroscience research, while Caltech construct $200 million biosciences complex.
Making It Personal
Cancer vaccine linked to increased immune response against leukemia cells.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!