Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Promega and BASF Develop Alternative Method on Identifying Allergenic Substances

Published: Thursday, April 11, 2013
Last Updated: Thursday, April 11, 2013
Bookmark and Share
Animal studies for cosmetic starting materials completely prohibited in the EU since March.

Promega and BASF have jointly developed an alternative method to animal studies that can reliably detect the allergenic potential of substances. Using a newly developed cell line, the reaction of skin cells to allergenic substances can now be demonstrated in the test tube.

Allergic skin reactions involve a variety of biochemical processes. These include the binding of a substance to one of the skin’s proteins and a resulting stress reaction. Both are early and reliable indicators of a possible allergenic potential of the test substance. In the newly developed cell line, scientists of RWTH Aachen University Hospital have modified a reporter gene from Promega, so that the stress reactions are coupled to a light signal. This gene construct was then stably inserted into human skin cell lines. Dr. Katarina Bohm, Marketing Manager of Promega: “Our luciferase vectors translate cellular changes into a luminescent signal. This allows stress reactions in skin cells to be easily detected with our luciferase assay systems.” The new cell line has been extensively tested at BASF and a standardized method has been developed to reliably assess the allergenic potential of a substance.

Previously, the skin sensitizing action of substances had to be determined in animal studies. “Combining the new method with two additional alternative methods to investigate skin sensitization allows us not only to significantly reduce the number of animal studies, but also to predict a possible allergic potential more reliably than before,” said Dr. Robert Landsiedel, Head of the short-term Toxicology unit at BASF. Developing the method at the present time is particularly important because following the REACH regulation, several thousand substances have to be tested for their skin sensitizing action by 2018. Furthermore, since March 2013 animal studies on cosmetic ingredients for the European market have been completely prohibited.

The alternative method was submitted to the European Commission (European Center for the Validation of Alternative Methods, ECVAM). This body determines whether it can be recognized as a standard method for toxicological studies in Europe.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Promega to Break Ground for New Processing and Manufacturing Facility
Facility will allow for efficiency improvements for processing, packaging, staging and shipping products.
Monday, July 07, 2014
Examine the Connection Between Consciousness and Nature
Experts explore consciousness through the perspective of intelligence in nature at the 2014 International Bioethics Forum: 3.8 Billion Years of Wisdom: Exploring the Genius of Nature.
Thursday, March 27, 2014
Rediscover Richard III, Explore the $1000 Genome and More at the 2013 International Symposium on Human Identification
24th International Symposium on Human Identification provides an opportunity for DNA analysts, members of law enforcement and other DNA industry professionals to learn about emerging forensic DNA techniques from experts in the field.
Thursday, August 15, 2013
Promega and MyBio Sign Exclusive Distribution Agreement
New partnership gives researchers and scientists in the University, Government and Commercial Laboratory Sector improved access to Promega’s world class product range.
Wednesday, June 12, 2013
The Society for Biomolecular Screening Awards Promega Scientist for Innovation
High Throughput Screening Award celebrates 'twinkles in the night'.
Tuesday, September 11, 2012
Promega on Target with 2014 Environmental Goals
Promega is on target described in the 2011 Corporate Responsibility Report.
Monday, August 13, 2012
Promega Corporation Building New Facility to Support Regulated Product Manufacturing
cGMP facility to accommodate business growth and customers who need FDA-regulated molecular biology products.
Tuesday, September 06, 2011
Promega Partners with ATCC to Offer New Cell Systems for Biologics Drug Discovery
Partnership for the cell banking and bioproduction of cells to create a new line of bioluminescence-based drug discovery assays for biologics.
Monday, June 27, 2011
Promega Expansion into Brazil Brings New Convenience and Support for Scientists
Now scientists in life science research, drug discovery and clinical diagnostics have direct access to technical service, fast delivery and the ability to request a quote online in Brazil.
Thursday, April 07, 2011
Promega SABiosciences Agreement Enables the Most Sensitive Tools for Pathway Analysis
The Companies combine their offerings to provide sensitive tools for interrogation specific cellular pathways via transcription factor reporters.
Tuesday, August 18, 2009
Promega Signs Agreement with the Guangzhou Institute of Biomedicine and Health
The agreement is designed to co-develop compound profiling solutions for small molecule and traditional Chinese medicine drug screening.
Wednesday, April 11, 2007
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Self-Assembling, Biomimetic Membranes May Aid Water Filtration
A synthetic membrane that self assembles and is easily produced may lead to better gas separation, water purification, drug delivery and DNA recognition, according to an international team of researchers.
Researchers Discover Immune System’s 'Trojan Horse'
Oxford University researchers have found that human cells use viruses as Trojan horses, transporting a messenger that encourages the immune system to fight the very virus that carries it.
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
How Cholesterol Leads to Clogged Arteries
A new study shows that when immune cells called neutrophils are exposed to cholesterol crystals, they release large extracellular web-like structures that trigger the production of inflammatory molecules linked to artherosclerosis.
Genetic Tug of War
Researchers have reported on a version of genetic parental control in mice that is more targeted, and subtle than canonical imprinting.
Ultrafast DNA Diagnostics
New technology developed by UC Berkeley bioengineers promises to make a workhorse lab tool cheaper, more portable and many times faster by accelerating the heating and cooling of genetic samples with the switch of a light.
Researchers Discover New Type of Mycovirus
Virus infects the fungus Aspergillus fumigatus, which can cause the human disease aspergillosis.
Error Correction Mechanism in Cell Division
Cell biologists have reported an advance in understanding the workings of an error correction mechanism that helps cells detect and correct mistakes in cell division early enough to prevent chromosome mis-segregation and aneuploidy, that is, having too many or too few chromosomes.
How to Become a Follicular T Helper Cell
Uncovering the signals that govern the fate of T helper cells is a big step toward improved vaccine design.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!