Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Hamilton’s Ion Chromatography Columns

Published: Tuesday, May 07, 2013
Last Updated: Monday, May 06, 2013
Bookmark and Share
Demonstrates excellent results and high flexibility.

In a study using ion chromatography, two of Hamilton’s anion exchange columns demonstrate excellent results. These columns provide reliable results in a variety of buffer compositions, lending themselves to be used in existing instrumentation and methodologies.

Ion chromatography is a reliable method for determining anion concentration in aqueous solutions or environmental samples.

The process analyzes standard inorganic ions typically using a carbonate-bicarbonate buffer as the mobile phase.

The affect of various buffer compositions with the stationary phase is the subject of numerous research discussions.

Test run: Hamilton’s separation columns with three common buffers

A study has now verified to what extent retention time is influenced by buffer composition. To test this, the three most widely used buffer compositions were used with two Hamilton ion chromatography columns.

The columns are part of a new Anion Line that the leading manufacturer of lab technology has developed as a solution for ion chromatography.

The tested columns were Anion Resolution, a high-resolution column with high sample loading capacity, and Anion Fast, developed for fast separation processes.

All Anion Line columns, including the two used in this study, are made of polyetheretherketone (PEEK) hardware. The stationary phase was a PRP-X100 resin that consists of cross-linked polystyrene-divinylbenzene particles functionalized with trimethyl ammonium anion exchange groups.

The Anion Resolution column was a 4.6 x 250 mm with a 7 µm particle size, while the Anion Fast column was 4.6 x 100 mm with a 5 µm particle size.

Test result: excellent results in all buffer compositions (0.1 mM NaSCN was added to all solvents)

In the test run carried out on a Dionex DX 500 ion chromatograph made by Thermo Fisher, both columns delivered excellent results in all three buffer compositions.

The Anion Resolution column (suitable especially for analyzing unknown samples) demonstrated high separation efficiency. The Anion Fast column used above is best for quickly analyzing samples with known components, giving an optimal ratio of good resolution and short analytical run times.

A benefit is that retention time differences between anions can be increased in unknown samples simply by reducing buffer concentration to prevent possible sample signal overlapping in high concentrations.

In fast analysis, on the other hand, the elution of the anions is accelerated by making eluent concentration stronger.

Further test results demonstrate that the quality of the results was independent from the buffer composition and the Anion Resolution and Anion Fast could be easily integrated into existing instrumentation without extra expense.

Chromatographers realize immediately benefit from the high performance of Hamilton columns.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Hamilton Storage Technologies Opens 52,000-Square-Foot Facility in Franklin, Massachusetts
Innovative open floor plan helps life science business address growing market demands.
Tuesday, January 22, 2013
Hamilton Robotics and French Police Scientifique Publish Paper Describing Large-Scale Production of Genetic Profiles
More than 300,000 profiles have already been processed on the Hamilton platforms and stored in French Database.
Wednesday, March 10, 2010
Hamilton Star Liquid Handler Installation at the Diamond Light Source MPL Laboratory
Hamilton Robotics has installed a STAR liquid handler at the commissioned Membrane Protein Crystallography Laboratory at the Diamond Light Source near Didcot in Oxfordshire.
Monday, June 11, 2007
Automation of Adherent Cell Culture Maintenance
Hamilton, Life & Brain and University of Bonn will jointly develop a system for the automated culture of primary cells, cell lines and embryonic stem cells.
Monday, May 14, 2007
Introduction of an Anti Droplet Control Pipette
HAMILTON introduces ADC, for pipetting volatile organic solvents, with pressure sensors in the pipetting channel to monitor each pipetting step.
Monday, December 11, 2006
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos