Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of Molecular Indexing™ technology. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adaptors chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
"We are pleased to introduce the first in a series of high-precision assays from Cellular Research Inc., enabling a new level of accuracy for identifying and counting distinct DNA molecules.", said Stephen Fodor, PhD, CEO of Cellular Research, Inc. “Bioo Scientific’s reputation for building high quality RNA Sequencing kits with strong customer service and support make them an ideal commercialization partner.”
All of the NEXTflex™ kits incorporate the proprietary “Enhanced Adapter Ligation Technology”, which offers the highest ligation efficiency available, resulting in longer and more diverse sequencing reads. Bioo Scientific’s robust enzymes ensure the highest quality libraries for global RNA profiling.