Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

High Yield RNA Isolation without the Paraffin Removal Step

Published: Wednesday, May 15, 2013
Last Updated: Wednesday, May 15, 2013
Bookmark and Share
AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

Using AMSBIO ExpressArt® RNA isolation technology it is now possible to achieve high RNA yield and maximum sequence length from FFPE or LCM samples without the paraffin removal step. ExpressArt® RNA technology overcomes traditional limitations placed on research due to limited sample size, RNA degradation and interference from the crosslinking in FFPE samples.

ExpressArt® FFPE RNA-ready isolation and mRNA amplification technology allows researcher to isolate higher quality and quantities of RNA from samples previously unsuitable for gene expression analysis. The kits includes patented de-cross linking technology which is uniquely able to isolate high quality RNA from old, archival FFPE samples (10 years old or more). The FFPE RNAready Kit provides special lysis and incubation conditions for optimal RNA recovery and improved template activity. Deparaffinisation of the tissue samples is followed by homogenisation and lysis by incubation with proteinase K and detergents. The lysis buffer is supplemented with the nucleic acid analogue NucleoGuard, which acts as universal inhibitor of RNases and displaces tightly bound RNA from high-molecular-weight aggregates, resulting in efficient release of RNA and preservation of the RNA quality. RNA solubilisation is followed by an innovative demodification step to reverse formalin induced cross-links and this enables the purified RNA to deliver maximal performance as template in reverse transcription reactions and subsequent downstream assays.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Ready-to-Use Tagged cDNA Clones
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Monday, June 01, 2015
Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
Saturday, January 31, 2015
AMSBIO Expands US Operations
Company announces opening of a new North American headquarters in Cambridge, MA.
Wednesday, April 23, 2014
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
Webinar Addresses 3D Cell-Based Models for Regenerative Medicine
Dr Elad Katz presents new on-demand webinar.
Friday, October 25, 2013
Webinar Discusses DNA Damage in Individual Cells
New on-demand webinar from AMSBIO.
Monday, September 30, 2013
AMSBIO & Sanguine Biosciences Announce Distribution Agreement
Agreement to distribute and support AMSBIO’s products and services throughout Europe.
Friday, August 23, 2013
AMSBIO Announces Expansion of Tissue Biorepository and Collection Services
Services to include blood products from human donors with auto-immune and neurological conditions.
Wednesday, April 24, 2013
Informative Guide to Gene Silencing & Delivery
AMSBIO’s new guide - 'Top Ten Tips for Gene Silencing & Delivery'.
Tuesday, June 19, 2012
Knockdown Cell Lines for DNA Repair Studies
New range of 20 DNA Repair Knockdown cell lines enables scientists to study the molecular etiology of tumour genomic instability and to exploit it in oncology research.
Wednesday, February 23, 2011
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Immunotherapy Agent Benefits Patients with Drug-Resistant Multiple Myeloma in First Human Trial
Daratumumab proved generally safe in patients, even at the highest doses.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!