Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

AB SCIEX Improves Food Safety with New Method to Identify Markers for Horse Meat

Published: Friday, May 24, 2013
Last Updated: Friday, May 24, 2013
Bookmark and Share
New LC/MS/MS-based meat speciation method detects numerous animal protein markers in a single analysis with higher accuracy and reliability than existing methods.

AB SCIEX announced that its scientists have developed a new method for detecting horse tissue present in meat samples. This is in response to recent reports, including the Food Standards Agency’s announcement earlier this year, that horse and pig DNA had been identified in beef products sold in several supermarket chains in Europe.

The new AB SCIEX method, which is based on LC/MS/MS (liquid chromatography / tandem mass spectrometry), represents a novel, more accurate approach to meat speciation. It detects the protein markers distinct to specific meat species and confirming the presence of a particular species in a sample by direct detection. The method also enables laboratories to detect veterinary drug residues in the same analysis.

While the method was optimized to identify horse tissue contamination in beef samples, it may also be adapted to detect peptide markers of numerous different animal types simultaneously. Ultimately, the method has the power to identify horse meat that may be present in other tissue samples (such as pork, beef or lamb) by its unique protein markers.

“Our new method shows a clear role for LC/MS/MS in meat speciation, giving scientists the most reliable results for identification of horse meat in food,” said Vincent Paez, Sr. Director for Food and Environmental at AB SCIEX. “It further demonstrates our commitment to helping companies and governments improve food safety and verify food authenticity.”

The horse meat markers that AB SCIEX scientists identified have been independently verified by research scientists at the University of Münster (Prof. Dr. Hans-Ulrich Humpf, Dr. Jens Brockmeyer and Christoph von Bargen). In addition, AB SCIEX scientists used the method in a recent LGC Standards proficiency testing scheme, confirming that the method can easily detect horse meat in beef at levels of 10% with no false positives. The method was then adapted to detect horse meat at levels as low as 1% in beef samples, making the method detection limits comparable to those achieved with existing methodologies such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) techniques.

The AB SCIEX mass spectrometry-based method offers a more accurate and reliable approach to meat speciation than PCR, which must detect the animal’s DNA, or ELISA, which must detect the intact animal proteins ‒ both of which have limitations. Additionally, this method is an improvement over other indirect LC/MS/MS-based methods, which target veterinary drug residues rather than the targeted animal proteins themselves. This new approach, however, does allow for the detection of veterinary drug residues in the same analysis, which is not possible by ELISA or PCR.

The new method was developed on an AB SCIEX LC/MS/MS platform, consisting of an AB SCIEX QTRAP® 5500 LC/MS/MS system coupled with an Eksigentekspert™ micro LC system. The QTRAP® 5500 system uses multiple reaction monitoring (MRM) to detect each peptide and is then capable of providing sequence information by acquiring a product ion scan for each triggered MRM, which can be used to confirm the peptide’s identity. This gives greater confidence for food testing when distinguishing between species. This is especially significant because, for example, horse and beef proteins may differ by as little as one or two amino acids.

“LC/MS/MS will allow laboratory scientists to analyse a single meat sample for 15-20 different animal species in a single run, with very little chance of false positives,” said Stephen Lock, PhD, Technical Market Applications Manager, Food & Environmental, AB SCIEX. “Future work is planned to add more markers to the method and further increase the number of species detected with this approach.”

As a leader in next-generation food testing technologies, AB SCIEX has previously developed similar methods for protein screening in food, including new techniques for detecting allergens such as eggs, milk, sesame seeds, nuts, and mustard simultaneously in food samples, as well as more recent work investigating casein detection in wine.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

SCIEX Works with the Francis Crick Institute and the University of Cambridge
New proteomic study to develop advanced resource for precision medicine research by using Microflow SWATH® Acquisition for HT industrialized proteomics.
Wednesday, June 29, 2016
Solutions for Biotherapeutic Characterization
Innovation to speed the routine.
Friday, June 17, 2016
SCIEX, QPS Holdings Collaborate
Agreement aims to advance the hybrid LBA-LCMS workflow for biologics quantification.
Wednesday, April 06, 2016
SCIEX, Children's Medical Research Institute Collaborate
SCIEX industrialized proteomics solutions, using SWATH® Acquisition-based workflows and powered by the OneOmics™ Project, will enable large scale proteome studies to advance cancer research.
Thursday, February 11, 2016
SCIEX, A*STAR’s BTI Launch Collaboration
Agreement aims to boost innovation of protein drugs that target new diseases and are safer for patients.
Wednesday, January 27, 2016
SCIEX, Pressure BioSciences Partner
SCIEX and Pressure BioSciences to offer complete solution for next-generation proteomics, to increase the reproducibility and depth of proteomic analysis.
Wednesday, January 13, 2016
SCIEX, University of Manchester Partner
SWATH® Acquisition-based next-generation proteomics technology promises to make industrial proteomics a reality.
Thursday, October 29, 2015
SCIEX Partners with the Paulovich Lab at Fred Hutchinson Cancer Research Center
Collaboration targets research reproducibility crisis with highly validated multiplex quantitation assays.
Wednesday, September 23, 2015
SCIEX and New Objective Announce Partnership
New nanospray sources to offer maximum sensitivity and robustness for NanoLC-MS.
Tuesday, June 02, 2015
SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
SCIEX and Hepregen Announce Co-Marketing Agreement
Alliance establishes SCIEX and Hepregen as a one-stop solution provider for drug metabolism investigators.
Friday, May 22, 2015
Sciex, Hepregen Team up on Metabolite Identification Offering
This recently announced collaboration aims to provide practical solutions for drug metabolism researchers.
Thursday, May 21, 2015
Dr. Gyula Vigh wins Arnold O. Beckman Award
SCIEX sponsored award recognizes outstanding contributions to the field of electrodriven separation techniques.
Thursday, May 07, 2015
SCIEX and Mass Consortium Corporation Announce Exclusive Reseller Agreement
SCIEX to provide customers with XCMSplus software solution for simplified and accelerated metabolomics workflows.
Tuesday, March 31, 2015
SCIEX Announces OneOmics™ Collaborators
Advaita Bioinformatics, Researchers at Yale University and ISB launch applications and libraries that combine next-generation proteomics and next-generation sequencing data in innovative ways.
Thursday, March 19, 2015
Scientific News
Open Source Seed Initiative – A Welcome Boost to Global Crop Breeding
A team of plant breeders, farmers, non-profit agencies, seed advocates, and policymakers have created the Open Source Seed Initiative.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Supplement May Switch off Cravings for High-Calorie Foods
Propionate is made by bacteria in the gut after they digest fiber, with researchers finding higher levels of the substance can curb cravings for junk food.
Implementation Science Approaches to Reduce Mother-to-Child HIV Transmission
The NIH study will investigate best practices to ease major disease burden in Sub-Saharan Africa.
Tough New Hydrogel Hybrid Doesn’t Dry Out
Water-based material could be used to make artificial skin, longer-lasting contact lenses.
New CAR T Cell Therapy Using Double Target Aimed at Solid Tumors
Researchers at Penn University have described how antibody, carbohydrate combination could apply to range of cancer types.
Lasers Carve the Path to Tissue Engineering
A new technique, developed at EPFL, combines microfluidics and lasers to guide cells in 3D space, overcoming major limitations to tissue engineering.
Link Between Canned Food, BPA Exposure Revealed
New Stanford research resolves the debate on the link between canned food and exposure to the hormone-disrupting chemical known as Bisphenol A, or BPA.
Portable Test Rapidly Detects Zika
To better diagnose and track the disease, scientists are now reporting a new $2 test that in the lab can accurately detect low levels of the virus in saliva.
Erasing Unpleasant Memories with a Genetic Switch
Researchers from KU Leuven and the Leibniz Institute for Neurobiology have managed to erase unpleasant memories in mice using a 'genetic switch'.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!