Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

AB SCIEX Improves Food Safety with New Method to Identify Markers for Horse Meat

Published: Friday, May 24, 2013
Last Updated: Friday, May 24, 2013
Bookmark and Share
New LC/MS/MS-based meat speciation method detects numerous animal protein markers in a single analysis with higher accuracy and reliability than existing methods.

AB SCIEX announced that its scientists have developed a new method for detecting horse tissue present in meat samples. This is in response to recent reports, including the Food Standards Agency’s announcement earlier this year, that horse and pig DNA had been identified in beef products sold in several supermarket chains in Europe.

The new AB SCIEX method, which is based on LC/MS/MS (liquid chromatography / tandem mass spectrometry), represents a novel, more accurate approach to meat speciation. It detects the protein markers distinct to specific meat species and confirming the presence of a particular species in a sample by direct detection. The method also enables laboratories to detect veterinary drug residues in the same analysis.

While the method was optimized to identify horse tissue contamination in beef samples, it may also be adapted to detect peptide markers of numerous different animal types simultaneously. Ultimately, the method has the power to identify horse meat that may be present in other tissue samples (such as pork, beef or lamb) by its unique protein markers.

“Our new method shows a clear role for LC/MS/MS in meat speciation, giving scientists the most reliable results for identification of horse meat in food,” said Vincent Paez, Sr. Director for Food and Environmental at AB SCIEX. “It further demonstrates our commitment to helping companies and governments improve food safety and verify food authenticity.”

The horse meat markers that AB SCIEX scientists identified have been independently verified by research scientists at the University of Münster (Prof. Dr. Hans-Ulrich Humpf, Dr. Jens Brockmeyer and Christoph von Bargen). In addition, AB SCIEX scientists used the method in a recent LGC Standards proficiency testing scheme, confirming that the method can easily detect horse meat in beef at levels of 10% with no false positives. The method was then adapted to detect horse meat at levels as low as 1% in beef samples, making the method detection limits comparable to those achieved with existing methodologies such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) techniques.

The AB SCIEX mass spectrometry-based method offers a more accurate and reliable approach to meat speciation than PCR, which must detect the animal’s DNA, or ELISA, which must detect the intact animal proteins ‒ both of which have limitations. Additionally, this method is an improvement over other indirect LC/MS/MS-based methods, which target veterinary drug residues rather than the targeted animal proteins themselves. This new approach, however, does allow for the detection of veterinary drug residues in the same analysis, which is not possible by ELISA or PCR.

The new method was developed on an AB SCIEX LC/MS/MS platform, consisting of an AB SCIEX QTRAP® 5500 LC/MS/MS system coupled with an Eksigentekspert™ micro LC system. The QTRAP® 5500 system uses multiple reaction monitoring (MRM) to detect each peptide and is then capable of providing sequence information by acquiring a product ion scan for each triggered MRM, which can be used to confirm the peptide’s identity. This gives greater confidence for food testing when distinguishing between species. This is especially significant because, for example, horse and beef proteins may differ by as little as one or two amino acids.

“LC/MS/MS will allow laboratory scientists to analyse a single meat sample for 15-20 different animal species in a single run, with very little chance of false positives,” said Stephen Lock, PhD, Technical Market Applications Manager, Food & Environmental, AB SCIEX. “Future work is planned to add more markers to the method and further increase the number of species detected with this approach.”

As a leader in next-generation food testing technologies, AB SCIEX has previously developed similar methods for protein screening in food, including new techniques for detecting allergens such as eggs, milk, sesame seeds, nuts, and mustard simultaneously in food samples, as well as more recent work investigating casein detection in wine.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More Than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Collaboration to Develop Cannabis Testing Standards
SCIEX workflow solution enables cannabis labs to ensure product safety with robust, cost-effective analytical methods to facilitate routine testing.
Tuesday, September 13, 2016
SCIEX Works with the Francis Crick Institute and the University of Cambridge
New proteomic study to develop advanced resource for precision medicine research by using Microflow SWATH® Acquisition for HT industrialized proteomics.
Wednesday, June 29, 2016
Solutions for Biotherapeutic Characterization
Innovation to speed the routine.
Friday, June 17, 2016
SCIEX, QPS Holdings Collaborate
Agreement aims to advance the hybrid LBA-LCMS workflow for biologics quantification.
Wednesday, April 06, 2016
SCIEX, Children's Medical Research Institute Collaborate
SCIEX industrialized proteomics solutions, using SWATH® Acquisition-based workflows and powered by the OneOmics™ Project, will enable large scale proteome studies to advance cancer research.
Thursday, February 11, 2016
SCIEX, A*STAR’s BTI Launch Collaboration
Agreement aims to boost innovation of protein drugs that target new diseases and are safer for patients.
Wednesday, January 27, 2016
SCIEX, Pressure BioSciences Partner
SCIEX and Pressure BioSciences to offer complete solution for next-generation proteomics, to increase the reproducibility and depth of proteomic analysis.
Wednesday, January 13, 2016
SCIEX, University of Manchester Partner
SWATH® Acquisition-based next-generation proteomics technology promises to make industrial proteomics a reality.
Thursday, October 29, 2015
SCIEX Partners with the Paulovich Lab at Fred Hutchinson Cancer Research Center
Collaboration targets research reproducibility crisis with highly validated multiplex quantitation assays.
Wednesday, September 23, 2015
SCIEX and New Objective Announce Partnership
New nanospray sources to offer maximum sensitivity and robustness for NanoLC-MS.
Tuesday, June 02, 2015
SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
SCIEX and Hepregen Announce Co-Marketing Agreement
Alliance establishes SCIEX and Hepregen as a one-stop solution provider for drug metabolism investigators.
Friday, May 22, 2015
Sciex, Hepregen Team up on Metabolite Identification Offering
This recently announced collaboration aims to provide practical solutions for drug metabolism researchers.
Thursday, May 21, 2015
Dr. Gyula Vigh wins Arnold O. Beckman Award
SCIEX sponsored award recognizes outstanding contributions to the field of electrodriven separation techniques.
Thursday, May 07, 2015
SCIEX and Mass Consortium Corporation Announce Exclusive Reseller Agreement
SCIEX to provide customers with XCMSplus software solution for simplified and accelerated metabolomics workflows.
Tuesday, March 31, 2015
Scientific News
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Charles River Acquires Agilux
Enhances Charles River’s early-stage capabilities in bioanalytical services.
Scientists Find Lethal Vulnerability in Treatment-Resistant Lung Cancer
The study describes how the drug Selinexor killed lung cancer cells and shrank tumors in mice when used against cancers driven by the aggressive and difficult-to-treat KRAS cancer gene.
How Baby’s Genes Influence Birth Weight And Later Life Disease
The large-scale study could help to target new ways of preventing and treating these diseases.
Genes Underlying Dogs’ Social Ability Revealed
The social ability of dogs is affected by genes that also seem to influence human behaviour, according to a new study from Linköping University in Sweden.
‘Cellbots’ Chase Down Cancer, Deliver Drugs Directly to Tumors
Programmable T cells shown to be versatile, precise, and powerful in lab studies.
Drug to Treat Alcohol Use Disorder Shows Promise Among Drinkers With High Stress
The findings suggest that potential future studies with drugs targeting vasopressin blockade should focus on populations of people with AUD who also report high levels of stress.
C Dots Show Powerful Tumor Killing Effect
Nanoparticles known as Cornell dots, or C dots, have shown great promise as a therapeutic tool in the detection and treatment of cancer.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!