Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

3D Spheroid Assays for Cancer Drug Screening

Published: Thursday, June 27, 2013
Last Updated: Thursday, June 27, 2013
Bookmark and Share
New tool to allow cell-based assays to be carried out in 3D.

AMSBIO has introduced a new range of 96-well format 3D Spheroid Cell Proliferation / Viability Assays, providing a new tool to allow cell-based assays to be carried out in 3D.

The new AMSBIO 3D Spheroid Proliferation/Viability Assay provides a useful tool for modeling tumor response in vitro.

The kit utilizes a 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells.

Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment.

Tumor spheroid expansion is visualized microscopically and can be quantitated through image analysis software for real-time and label free evaluation.

At the conclusion of the assay, cell viability may be assessed by fluorescence using Resazurin.

The 3D Spheroid Fluorometric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitating cell viability within the spheroids in response to pharmacological treatment.

The AMSBIO 3D Spheroid Proliferation/Viability Assay establishes physiological gradients for nutrients, oxygen, catabolites, and pH due to limitations in diffusion through multicellular layers.

These gradients also promote the creation of heterogeneous cell populations with proliferating cells on the spheroid surface, quiescent cells in the intermediate layers, and necrotic cells in the core, similar to an avascular tumor.

Uniform spheroid size and physiology is determined through cell seeding, providing a robust and reproducible assay format for drug screening or pathway analysis.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Ready-to-Use Tagged cDNA Clones
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Monday, June 01, 2015
Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
Saturday, January 31, 2015
AMSBIO Expands US Operations
Company announces opening of a new North American headquarters in Cambridge, MA.
Wednesday, April 23, 2014
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
Webinar Addresses 3D Cell-Based Models for Regenerative Medicine
Dr Elad Katz presents new on-demand webinar.
Friday, October 25, 2013
Webinar Discusses DNA Damage in Individual Cells
New on-demand webinar from AMSBIO.
Monday, September 30, 2013
AMSBIO & Sanguine Biosciences Announce Distribution Agreement
Agreement to distribute and support AMSBIO’s products and services throughout Europe.
Friday, August 23, 2013
AMSBIO Announces Expansion of Tissue Biorepository and Collection Services
Services to include blood products from human donors with auto-immune and neurological conditions.
Wednesday, April 24, 2013
Informative Guide to Gene Silencing & Delivery
AMSBIO’s new guide - 'Top Ten Tips for Gene Silencing & Delivery'.
Tuesday, June 19, 2012
Knockdown Cell Lines for DNA Repair Studies
New range of 20 DNA Repair Knockdown cell lines enables scientists to study the molecular etiology of tumour genomic instability and to exploit it in oncology research.
Wednesday, February 23, 2011
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos