Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Crystal Structure of a Human IaB Kinase ß Asymmetric Dimer

Published: Thursday, June 27, 2013
Last Updated: Thursday, June 27, 2013
Bookmark and Share
The observed domain movements in the structures of IKK may represent transphosphorylation steps that accompany IKK activation.

SUMMARY

Phosphorylation of inhibitor of nuclear  transcription factor B (IB) by IB  kinase (IKK) triggers the degradation of  IB and migration of cytoplasmic B to  the nucleus where it promotes the  transcription of its target genes.  Activation of IKK is achieved by phosphorylation of its main subunit,  IKK at the activation loop sites. Here  we report the 2.8 Å resolution crystal  structure of human IKKhIKK which  is partially phosphorylated and bound to the staurosporine analog K252a. The  hIKK protomer adopts a trimodular  structure that closely resembles that from  Xenopus laevis (xIKK: an N-terminal  kinase domain (KD), a central ubiquitinlike domain (ULD), and a C-terminal  dimerization domain (SDD). Although  hIKK and xIKK utilize a similar  dimerization mode, their overall  geometries are distinct. In contrast to the  structure resembling closed shears  reported previously for xIKK hIKK exists as an open asymmetric dimer in  which the two KDs are further apart,  with one in an active and the other in an  inactive conformation. Dimer  interactions are limited to the C-terminal  six-helix bundle that acts as a hinge  between the two subunits. The observed  domain movements in the structures of  IKK may represent transphosphorylation steps that accompany  IKK activation.

This article was published online in The Journal of Biological Chemistry and is free to access.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 4,000+ scientific posters on ePosters
  • More Than 5,300+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Solvent-free, Catalyst-free Peracetylation of Natural Products
In this study, a tunable microwave-assisted method for the solvent-free and catalyst-free peracetylation of natural products was developed.
Tuesday, November 22, 2016
Sonochemical Reactors
A critical analysis of the underlying mechanisms for intensification, available reactor configurations and overview of the different applications exploited successfully are presented.
Tuesday, November 22, 2016
Economical and Scalable Synthesis of 6-amino-2-cyanobenzothiazole
An economical and scalable synthesis of ACBT is presented, along with advantages for scale-up over previously reported routes.
Tuesday, November 22, 2016
Identifying Functional Biomarkers of Traumatic Brain Injury
In this study, prefrontal hemodynamic biomarkers were identified that can distinguish traumatic brain injury patients from healthy subjects.
Friday, November 18, 2016
Enhancing On-line Biological Sample Processing
New techniques are required to meet emerging demands on biopharmaceutical analysis.
Wednesday, November 09, 2016
A Simple Tool for Toxicological Analysis
In this study, GC-MS is used for the determination of clozapine, and five antidepressants in human plasma, serum and whole blood.
Friday, October 28, 2016
Identification of Individual Red Blood Cells by Raman Microspectroscopy
In this study, Raman Microspectroscopy was used to identify individual red blood cells.
Friday, October 28, 2016
Targeting Cannabinoid CB2 Receptors in the CNS
With endogenous cannabinoids considered as a potential target to combat CNS diseases, this article examines the role of CB2R could play in fighting some disorders.
Wednesday, October 19, 2016
Food Analysis Applications of Core-Shell Columns in HPLC
Despite applications of core-shell particles columns in food analysis being at an early stage, articles describing their use for improving separations of several classes of compounds are becoming more frequent.
Wednesday, October 19, 2016
Porphyrins as Catalysts in Scalable Organic Reactions
This review covers the most relevant scalable porphyrin-catalysed procedures, showing how these compounds represent broad applications in chemistry.
Tuesday, July 26, 2016
Determination of 13 Organic Toxicants in Human Blood
Researchers have utilised liquid-liquid extraction coupling HPLC-MS/MS to identify and quantify organic toxicants in human blood.
Friday, July 22, 2016
A Novel Cell Culture Model For Forensic Biology Experiments
Researchers have developed a new cell culture model which provides an efficient research tool in forensic biology.
Friday, July 22, 2016
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Friday, June 24, 2016
Plasma Biomarkers for Breast Cancer Diagnosis
Plasma lipidomics profiling identified lipid biomarkers in distinguishing early-stage breast cancer from benign lesions.
Wednesday, May 11, 2016
A Mechanistic Tool for Studying Fungal Pathogens
This study presents an overview of lipid extraction, chromatographic separation and mass spectrometry employed in qualitative and quantitative sphingolipidomics in fungi.
Wednesday, May 11, 2016
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Soil Carbon Release Might Equal U.S. Emissions
Research suggests 55M tons of carbon will be release from soils by 2050, 17% higher than prjected emissions.
‘NoBody,’ a Microprotein On a Mission
Researchers identify over 400 microproteins encoded in the human genome, one of which clears unneeded genetic material inside cells.
Unexpected Epigenetic Enzymes Role in Cancer
Researchers use epigenetics to identify the role of an enzyme family as regulators of genetic message interpretation in yeast.
Genetic Links to Brain Cancer Cell Growth
Researchers discover clues to tumour behaviour from genetic differences between brain cancer cells and normal tissue cells.
New Form of Autism Found
An international team of researchers have identified a new form of syndromic autism.
Radiation-Free Imaging in the Brain
Scientists create sensors that use proteins to detect particular targets through induced blood flow changes.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!