Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Streamlining PCR Assay Design with the IDT PrimerQuest® Design Tool

Published: Wednesday, July 03, 2013
Last Updated: Wednesday, July 03, 2013
Bookmark and Share
Free online tool enhances PCR primer and probe design with new upgrades.

Integrated DNA Technologies (IDT) has launched a new and enhanced version of the open access PrimerQuest® online tool, for efficient PCR primer and probe design.

Selection parameters have been optimized with built-in presets for a diverse range of applications, to produce the very best primers and probes.

Compatibility with multiple sequence entry formats has been expanded and combined with a more intuitive user interface. These features streamline the process of setting up a PCR or qPCR experiment for both new and existing users.

The PrimerQuest design tool is ideal for a variety of sequencing and endpoint PCR applications, with updated default parameter pre-sets (GC clamp, Poly X and Pair max difference Tm), and overall improved parameter criteria for primer/probe size, Tm and GC%.

As the tool of choice for custom design applications, the PrimerQuest design tool, which is based on Primer 3, delivers designs derived from user-specified information such as reaction conditions (e.g., MgCl2 and dNTP concentrations).

Users can designate target locations, exon junctions or existing primer and probe locations to effortlessly obtain designs for specific experimental requirements.

In addition, the PrimerQuest tool can now be used for studies on any scale, supporting larger experimental designs via the batch load setting.

This feature can be used to analyze up to 50 sequences at a time, and primers can now be ordered in plate format for streamlining high-throughput PCR studies.

Integration of the PrimerQuest online tool with other software programs has been improved for maximum compatibility. Sequences can now be uploaded in Excel format, or can even be manually entered and manipulated.

Downloading in FASTA format using GenBank Accession ID allows efficient transfer and analysis, while a link out enables BLAST search of primers to quickly ensure specificity.

These new features are now more easily accessible with the updated user interface, incorporating a modernized look and easy one-click solutions, for intuitive control and selection of parameters.

With the updated PrimerQuest software, users are guaranteed maximum performance in PCR and qPCR experiments.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 4,000+ scientific posters on ePosters
  • More Than 5,300+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

IDT Supports Brazilian Research Team’s Biocontrol Method for Zika Virus
The research team has developed a natural method for controlling mosquito-transmitted diseases, including dengue, Zika, and chikungunya.
Friday, August 05, 2016
IDT Acquires MBiotech
Company has acquired MBiotech to expand global sales distribution net in South Korea.
Friday, June 03, 2016
TBUSA, IDT Announce Collaboration
Collaboration aims to support targeted RNA Sequencing.
Wednesday, April 27, 2016
IDT Acquires AITbiotech
Acquisition increases capacity to deliver high quality, locally manufactured oligos .
Thursday, December 10, 2015
IDT Expands Oligonucleotide Manufacturing in Singapore
Acquisition increases capacity to deliver high quality, locally manufactured oligos.
Wednesday, December 02, 2015
Dr Patricia Tavormina Named Winner of First IDT ISO 14001 Sustainability Award
Biodiversity research on methane sinks and global warming earns $14,001 in oligo credit.
Thursday, September 10, 2015
Untangling the Web of Gene Expression
New technology for understanding gene expression drives research forward.
Wednesday, May 20, 2015
Fast and Focused Sequencing from Single Genes to Entire Exomes
IDT reveals how to increase sequencing success with new and existing enrichment panels.
Monday, March 16, 2015
Discover Affordable Custom NGS Panels
IDT webinar helps NGS researchers bring cost-savings to target capture panels.
Thursday, February 12, 2015
Synthetic Biology Webinar - Genome Modifications Made Easy
IDT reveals how to overcome the challenges of CRISPR-mediated genome editing.
Thursday, January 15, 2015
IDT and Ubiquitome Partner to Develop Mobile Ebola Test
Handheld, battery-powered Freedom4 enables rapid field deployment of gold standard molecular test.
Wednesday, December 03, 2014
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Saturday, November 08, 2014
IDT Shares Tips for Optimizing Target Capture
The article discusses improved target capture methods for increasing reliability of next generation sequencing data.
Friday, October 24, 2014
From Honey Bees to Hearts - Keep Track of the Latest in NGS
Free IDT symposium explores diverse NGS applications.
Wednesday, September 24, 2014
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Tuesday, September 09, 2014
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Biological Link between the Gut Microbiome and Parkinson’s Disease
The findings suggest that targeting the gut microbiome may provide a new approach for diagnosing and treating Parkinson’s disease.
Study Reveals New Role for Hippo Pathway in Suppressing Cancer Immunity
Hippo pathway signaling regulates organ size by moderating cell growth, apoptosis and stem cell renewal, but dysregulation contributes to cancer development.
Genetic Links to Brain Cancer Cell Growth
Researchers discover clues to tumour behaviour from genetic differences between brain cancer cells and normal tissue cells.
RNAi Activated in Response to Influenza
Discovery could lead to better ways of combating serious infections, including Ebola and Zika.
Unexpected Epigenetic Enzymes Role in Cancer
Researchers use epigenetics to identify the role of an enzyme family as regulators of genetic message interpretation in yeast.
Allen Institute Releases Gene Edited Human Stem Cell Lines
The Allen Cell Collection, a publicly available collection of gene edited pluripotent stem cells, has been made available by the Allen Institute.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!