Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Streamlining PCR Assay Design with the IDT PrimerQuest® Design Tool

Published: Wednesday, July 03, 2013
Last Updated: Wednesday, July 03, 2013
Bookmark and Share
Free online tool enhances PCR primer and probe design with new upgrades.

Integrated DNA Technologies (IDT) has launched a new and enhanced version of the open access PrimerQuest® online tool, for efficient PCR primer and probe design.

Selection parameters have been optimized with built-in presets for a diverse range of applications, to produce the very best primers and probes.

Compatibility with multiple sequence entry formats has been expanded and combined with a more intuitive user interface. These features streamline the process of setting up a PCR or qPCR experiment for both new and existing users.

The PrimerQuest design tool is ideal for a variety of sequencing and endpoint PCR applications, with updated default parameter pre-sets (GC clamp, Poly X and Pair max difference Tm), and overall improved parameter criteria for primer/probe size, Tm and GC%.

As the tool of choice for custom design applications, the PrimerQuest design tool, which is based on Primer 3, delivers designs derived from user-specified information such as reaction conditions (e.g., MgCl2 and dNTP concentrations).

Users can designate target locations, exon junctions or existing primer and probe locations to effortlessly obtain designs for specific experimental requirements.

In addition, the PrimerQuest tool can now be used for studies on any scale, supporting larger experimental designs via the batch load setting.

This feature can be used to analyze up to 50 sequences at a time, and primers can now be ordered in plate format for streamlining high-throughput PCR studies.

Integration of the PrimerQuest online tool with other software programs has been improved for maximum compatibility. Sequences can now be uploaded in Excel format, or can even be manually entered and manipulated.

Downloading in FASTA format using GenBank Accession ID allows efficient transfer and analysis, while a link out enables BLAST search of primers to quickly ensure specificity.

These new features are now more easily accessible with the updated user interface, incorporating a modernized look and easy one-click solutions, for intuitive control and selection of parameters.

With the updated PrimerQuest software, users are guaranteed maximum performance in PCR and qPCR experiments.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Dr Patricia Tavormina Named Winner of First IDT ISO 14001 Sustainability Award
Biodiversity research on methane sinks and global warming earns $14,001 in oligo credit.
Thursday, September 10, 2015
Untangling the Web of Gene Expression
New technology for understanding gene expression drives research forward.
Wednesday, May 20, 2015
Fast and Focused Sequencing from Single Genes to Entire Exomes
IDT reveals how to increase sequencing success with new and existing enrichment panels.
Monday, March 16, 2015
Discover Affordable Custom NGS Panels
IDT webinar helps NGS researchers bring cost-savings to target capture panels.
Thursday, February 12, 2015
Synthetic Biology Webinar - Genome Modifications Made Easy
IDT reveals how to overcome the challenges of CRISPR-mediated genome editing.
Thursday, January 15, 2015
IDT and Ubiquitome Partner to Develop Mobile Ebola Test
Handheld, battery-powered Freedom4 enables rapid field deployment of gold standard molecular test.
Wednesday, December 03, 2014
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Saturday, November 08, 2014
IDT Shares Tips for Optimizing Target Capture
The article discusses improved target capture methods for increasing reliability of next generation sequencing data.
Friday, October 24, 2014
From Honey Bees to Hearts - Keep Track of the Latest in NGS
Free IDT symposium explores diverse NGS applications.
Wednesday, September 24, 2014
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Tuesday, September 09, 2014
IDT Shares Comprehensive qPCR Resources
Special qPCR compendium issue of DECODED now freely available.
Wednesday, August 06, 2014
IDT Acquires SURVEYOR® Nuclease Business from Transgenomic
Addition of SURVEYOR enzyme and kits expands IDT's molecular biology product offerings.
Wednesday, July 02, 2014
World Cup 2014 - with a Scientific Twist
IDT fuses fruit flies, football and fun in a unique global contest with great prizes.
Thursday, June 12, 2014
Summit Partners Makes Growth Equity Investment in IDT
IDT’s founder, Dr. Joseph Walder, will remain the company’s majority owner.
Wednesday, March 19, 2014
Enhanced Molecular Inversion Probes for NGS Target Enrichment
IDT presents latest developments at 15th annual Advances in Genome Biology and Technology meeting.
Tuesday, February 04, 2014
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos