Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Olympus Introduces 40x Silicone Oil Microscope Objective

Published: Friday, September 13, 2013
Last Updated: Friday, September 13, 2013
Bookmark and Share
Offers brighter, high-resolution live cell and time-lapse imaging.

Olympus has introduced a 40x silicone oil microscope objective, its latest innovation in silicone immersion microscope optics.

The new objective fills out the company’s full line of silicone oil optics, which already includes popular 30x and 60x objectives designed for live cell and time-lapse imaging.

Olympus silicone oil objectives can markedly improve optical performance for live cell confocal, widefield fluorescence, multiphoton, differential interference contrast (DIC) and other applications.

Both the new intermediate-magnification UPLSAPO 40x and the existing UPLSAPO 60x objective are designed specifically to work with the Olympus Zero Drift continuous autofocus system for extended time-lapse imaging.

In addition, all three of the objectives are compatible with protocols that use repeat-single-shot autofocus.

In contrast to water immersion objectives, silicone oil optics are useful in long-term imaging experiments where water evaporation is a pervasive issue.

While water immersion lenses are traditionally used to reduce refraction index mismatch, they are not practical for long-term, time-lapse imaging because of moisture loss and low viscosity.

Compared to conventional oil immersion objectives, the new 40x, 1.25 numericalaperture (NA), 0.3mm working distance objective improves resolution and reduces loss of contrast due to spherical aberration.

By reducing the mismatch between the refractive index of the specimen and that of the immersion medium into which the tip of the objective is dipped, silicone oil provides higher resolution and brightness, especially when using the microscope to image into thick samples.

Glycerol immersion optics are another option, but glycerol is sometimes not ideal because it tends to draw moisture from the air, resulting in changes in refractive index over time.

Silicone oil is very stable and does not have any of these issues; in addition, it more closely matches the refractive index (N=1.404) of intracellular components, making the new objective useful for imaging into cells during for long-term, time-lapse experiments.

The silicon objective has a correction collar, so users can correct for the spherical aberration that is present when imaging into a specimen beyond a cover slip.

Image resolution and contrast, along with fluorescence performance, are optimized and maintained by adjusting this collar.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Imaging Companies in Europe Shoulder-to-Shoulder with Life Scientists
Imaging companies in Europe get closer to scientists by founding an open Board linked to the upcoming European research infrastructure Euro-BioImaging.
Saturday, November 15, 2014
Olympus VS120 Virtual Slide Scanning System Earns Three Awards at International Scanner Contest
Unrivalled performance in fluorescence slide scanning.
Tuesday, November 13, 2012
Judges Named for 2012 Olympus BioScapes Digital Imaging Competition®
Competition carries a top prize valued at $5,000.
Thursday, October 11, 2012
Olympus America Inc. and Caris Diagnostics Partner to Unveil New Pathology Reporting Tool
Olympus EndoWorks is a robust, web-based information management solution that spans all stages of GI and pulmonary patient care.
Monday, December 19, 2011
Photo of Water Creature Resembling a Mouse Earns First Prize
At last, a mouse that says ‘Cheese’. A photo of a curious underwater life form that bears a striking resemblance to a cartoon mouse has earned first prize in the 2011 Olympus BioScapes Digital Imaging Competition®.
Friday, November 18, 2011
Olympus Canada to Distribute the new Hitachi TM3000 Table Top SEM
New partnership makes Olympus the exclusive distributor of the new TM3000 Table Top Scanning Electron Microscope in Canada.
Monday, April 19, 2010
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!