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CYTOO’s 2D+ and 3D+ Cell Culture Platform at MipTec 2013

Published: Monday, September 23, 2013
Last Updated: Monday, September 23, 2013
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CYTOO will present the 2D+ and 3D+ Cell Culture Platforms, opening fresh perspectives for cell based assay development in drug discovery.

2D+ Technology is based on CYTOO’s adhesive micropatterns in 96-well plates which guide cell architecture and behaviour. It addresses a major concern with traditional 2D culture, in which cells spread and move in an uncontrolled manner, introducing a considerable but unnoticed variability in cell function. By defining the 2D topology of cell adhesion, the 2D+ Cell Culture Platform enables fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations. This approach results in control of cell contractility, cell polarity, organelle positioning, and cell division axis.

The 3D+ Cell Culture is the new generation of 3D technologies. It enables short and long-term assays: hundreds of identical structures can be grown in an in vivo-like microenvironment and in microplate format, which facilitates the identification and quantification of even rare phenotypes. Micropatterned acini cultures can quickly and conveniently be created with any epithelial immortalized cell line or primary cells that have the capacity to form polarized acini in a 3D matrix.

The 3D+ Cell Culture is a simple method to screen drugs in a polarized and 3D automation context. It opens new perspectives in oncology, in the understanding of lumen initiation and in the approach of several conditions such as ciliopathies, gene mutations and diabetes.

During MipTec, CYTOO will be Gold Sponsor for the Stem Cells in Biomedicine Scientific Forum, held on September 24. Sébastien Degot, Senior R&D Scientist, will then present the “Phenotypic profiling using probabilistic density maps generated from micropatterned cells”.

During the Thermo Scientific Industrial Symposium, held on September 26, he will also present the “advantages of CYTOO’s 2D+/3D+ Cell Culture Platform for the imaging and analysis of complex cell models: assessment using the Thermo Scientific Cellomics CellInsight”.


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