Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Discussing the Science of Big DNA

Published: Monday, September 23, 2013
Last Updated: Monday, September 23, 2013
Bookmark and Share
Read about the most recent advances in synthetic biology in the latest issue of DECODED.

In the latest issue of DECODED, the quarterly newsletter from Integrated DNA Technologies (IDT), Dr Dan Gibson discusses the science of big DNA. Dr Gibson spent most of the last decade working at the J Craig Venter Institute, as well as Synthetic Genomics Inc. (SGI), to develop, establish, and commercialize methods to take synthetic biology beyond its current limitations. In 2010 Dr Gibson and his team produced a DNA construct of 1.1 Mb, the largest synthetic DNA sequence to date, something which had not been achievable using traditional restriction/ligation methodologies. Instead, the team developed the Gibson Assembly™ Method, which uses a unique enzyme mix to assemble DNA elements with 20–80 overlapping bases. Once reamplified, the population, enriched with the correct sequence, is much faster to screen. The technology makes possible the creation of 10–30 kb sequences—the size of an entire biological pathway—in less than a week, and without cloning into E. coli. Dr Gibson and his team are currently looking at ways in which they can refine DNA assembly methods; improve large gene delivery to host cells; and develop methods that rely less on host organisms for copying their synthetic constructs.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Untangling the Web of Gene Expression
New technology for understanding gene expression drives research forward.
Wednesday, May 20, 2015
Fast and Focused Sequencing from Single Genes to Entire Exomes
IDT reveals how to increase sequencing success with new and existing enrichment panels.
Monday, March 16, 2015
Discover Affordable Custom NGS Panels
IDT webinar helps NGS researchers bring cost-savings to target capture panels.
Thursday, February 12, 2015
Synthetic Biology Webinar - Genome Modifications Made Easy
IDT reveals how to overcome the challenges of CRISPR-mediated genome editing.
Thursday, January 15, 2015
IDT and Ubiquitome Partner to Develop Mobile Ebola Test
Handheld, battery-powered Freedom4 enables rapid field deployment of gold standard molecular test.
Wednesday, December 03, 2014
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Saturday, November 08, 2014
IDT Shares Tips for Optimizing Target Capture
The article discusses improved target capture methods for increasing reliability of next generation sequencing data.
Friday, October 24, 2014
From Honey Bees to Hearts - Keep Track of the Latest in NGS
Free IDT symposium explores diverse NGS applications.
Wednesday, September 24, 2014
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Tuesday, September 09, 2014
IDT Shares Comprehensive qPCR Resources
Special qPCR compendium issue of DECODED now freely available.
Wednesday, August 06, 2014
IDT Acquires SURVEYOR® Nuclease Business from Transgenomic
Addition of SURVEYOR enzyme and kits expands IDT's molecular biology product offerings.
Wednesday, July 02, 2014
World Cup 2014 - with a Scientific Twist
IDT fuses fruit flies, football and fun in a unique global contest with great prizes.
Thursday, June 12, 2014
Summit Partners Makes Growth Equity Investment in IDT
IDT’s founder, Dr. Joseph Walder, will remain the company’s majority owner.
Wednesday, March 19, 2014
Enhanced Molecular Inversion Probes for NGS Target Enrichment
IDT presents latest developments at 15th annual Advances in Genome Biology and Technology meeting.
Tuesday, February 04, 2014
Not a Constant Value: Understanding Tm
IDT explains how to optimize oligo hybridization-based protocols.
Friday, December 20, 2013
Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
How a Kernel Got Naked and Corn Became King
Ten thousand years ago, a golden grain got naked, brought people together and grew to become one of the top agricultural commodities on the planet.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!