Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Zetasizer APS Analyzes Sticky Proteins with No Cross-Contamination

Published: Monday, October 14, 2013
Last Updated: Sunday, October 13, 2013
Bookmark and Share
New application note published on Malvern Instruments website.

A new application note published on the Malvern Instruments website demonstrates the absence of any cross contamination when using the company’s Zetasizer APS (Auto Plate Sampler) to analyze known ‘sticky’ proteins.

The Zetasizer APS dynamic light scattering (DLS) system enables the automated measurement of multiple samples and has wide application in the rapid screening of proteins.

The risk of cross-contamination is a concern to many people when considering automated analysis in a multiwell format and the experiment was designed to thoroughly test the instrument’s capabilities. The application note can be downloaded at

Setup and operation of the Zetasizer APS is straightforward and its stringent cleaning protocols are designed to ensure that no cross contamination occurs.

The work described in the new application note used familiar protein samples to demonstrate the lack of detectable cross-contamination.

Samples included insulin, in both “monomeric” (the insulin hexamer) and “oligomeric” forms, and Abeta, the amyloid beta peptide known for its stickiness and tendency to adhere to plastic. The Abeta sample was present in two forms, fibrillar and oligomeric.

The order in which samples were loaded onto the system was chosen so as to maximize the risk of cross-contamination.

The oligomeric insulin was dispensed first using a standard pipette, followed by the small and pure insulin, and then the larger polydisperse Abeta samples.

Since large protein aggregates scatter much more light than small pure protein samples, if cross-contamination were to occur the polydisperse Abeta sample would be detected within the small homogenous protein sample. The results show that after running multiple tests, there was no detectable cross contamination.

The Zetasizer APS delivers the same high sensitivity, high specification DLS measurements as other systems in Malvern’s established Zetasizer family.

The automated processing of samples in multiwell plates enables the generation of high quality DLS data with no user intervention, a major benefit in maximizing productivity.

The system’s highly practical data display capability allows easy retrieval of results of interest, eliminating the need to manually scan through all measurements. In addition, the Zetasizer APS plate navigator feature acts as a data mapping tool, for rapid screening of results to retrieve the information of most interest and subsequent in-depth investigation of selected data sets.

The separate temperature controls of the plate holder and the measurement cell allow the Zetasizer APS to maintain the protein samples in optimal condition until measurement. In addition, thermal trend measurements between 2°C to 90°C with 0.1°C degree precision can be defined.

The plate scheduler allows the user to graphically set up size and thermal trend measurements of various samples from the same plate using a variety of different SOPs.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

New Partnership Between Malvern Instruments and RheoSense
Distribution agreement brings m-VROCi to industrial markets.
Tuesday, March 04, 2014
Component-Specific Particle Characterization of Pharmaceutical Cream
Publication shows use of Morphologi G3-ID for component-specific particle characterization of pharmaceutical cream.
Wednesday, January 29, 2014
Malvern Signs Exclusive Agreement with Affinity Biosensors
Agreement to distribute Affinity Biosensors’ Archimedes system extends Malvern Instruments’ biopharma solutions.
Tuesday, March 05, 2013
Malvern Team Presents Poster at ACS Colloids 2012 Symposium
Dr Ana Morfesis and Dr Ulf Nobbmann will present a poster on ‘Nanoparticle characterization by light scattering’.
Friday, May 18, 2012
Malvern takes ACHEMA Visitors from R&D Through to Product QC
Visitors to the Malvern Instruments stand at ACHEMA 2012 will see how the company’s range of complementary materials characterization technologies and instrumentation support the needs of manufacturing industry.
Wednesday, March 28, 2012
Malvern Instruments Opens New Multipurpose Facility in Houston
At 22,000 sq. ft. the new building is double the size of Malvern’s previous premises in Houston and houses state-of-the-art laboratories, product development, manufacturing and commercial operations.
Tuesday, March 27, 2012
Malvern Instruments and Postnova Analytics Co-operate
Both companies co-operate to deliver combined solution for characterizing challenging particulate systems.
Friday, February 17, 2012
Malvern Rheology Expert Contributes Chapter to Handbook of Nutraceuticals
The Handbook of Nutraceuticals Vol 2 is now available on-line as an e book.
Thursday, January 19, 2012
Extreme droplet sizing! Malvern Spraytec excels in zero gravity testing
ADA Technologies has confirmed that both NASA’s new space-bound fire extinguisher and the Spraytec laser diffraction spray particle size analyzer from Malvern Instruments function in zero gravity just as well as they do on Earth.
Tuesday, December 06, 2011
Malvern Scientists will lead Protein Characterization Workshop at PEGS Summit
Drs Kevin Mattison, Ulf Nobbmann and Jason Sanchez will lead a workshop at the 7th Annual PEGS.
Tuesday, September 13, 2011
Malvern Instruments and Purdue University Present Joint Symposium on Light Scattering Techniques for Nanoparticle Characterization
The symposium will take place on the Purdue Campus at West Lafayette, Indiana.
Thursday, September 08, 2011
Malvern Instruments and Paraytec Enter into Development and Technology Licensing Agreement
Paraytec’s ActiPix® technology being added to Malvern’s materials characterization portfolio.
Thursday, July 21, 2011
‘Two in a row’ as Malvern Instruments wins Queen’s Award for Second Year in Succession
Malvern Instruments is celebrating winning the Queen’s Award for Enterprise in the category of International Trade in two successive years.
Tuesday, May 17, 2011
Malvern Specialist Discusses Light Scattering Techniques at BioProcess International Europe 2011
Dr Hanna Jankevics will present a poster at the 7th annual BioProcess International Europe conference in Nice (France).
Thursday, April 14, 2011
Malvern Instruments Joins SoftComp Consortium and ESMI Project
Aim is to promote excellence in cross-disciplinary research and technique development in soft matter composites and complex fluids.
Monday, March 21, 2011
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
World’s First Therapeutic Venom Database
Open-source library describes nearly 43,000 effects on the human body.
Biologists Induce Flatworms to Grow Heads and Brains of Other Species
Findings shed light on role of a new kind of epigenetic signaling in evolution, could yield clues for understanding birth defects and regeneration.
Fat Cells Originating from Bone Marrow Found in Humans
Cells could contribute to diabetes, heart disease.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos