Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

New Super-Resolution System for 3D Localization Microscopy

Published: Friday, October 18, 2013
Last Updated: Friday, October 18, 2013
Bookmark and Share
Leica SR GSD 3D - Highly precise and reproducible localization of single molecules and cellular structures in three dimensions.

Super-resolution is a major trend in light microscopy. Extending its portfolio of super-resolution microscopy products, Leica has now launched the Leica SR GSD 3D, a widefield system that not only offers 2D, but now also 3D super-resolution imaging of molecules and cellular structures.

Based on GSD (Ground State Depletion) or dSTORM technology, the widefield fluorescence microscope attains resolutions of down to 20 nm in the lateral and 50 nm in the axial direction.

The Leica SR GSD 3D also scores with its unique precision for localizing single molecules, its system stability and its optical performance. These attributes are essential for reproducible, high-quality results in a very short time.

The benefits of the Leica SR GSD 3D are rounded off by the easy operation of the fully automated system and software.

The Leica SR GSD 3D is equipped with a 160x high-performance objective specifically developed for super-resolution microscopy. Its design is optimized for high-power laser emissions.

The extremely low autofluorescence ensures a high signal-to-noise ratio, which is ideal for single molecule detection applications.

To enable the localization of molecules in z direction, i.e. above and below the focal plane, the Leica SR GSD 3D uses the astigmatism effect. Astigmatism is actually an image aberration, but is created deliberately here with a cylindrical lens.

Corresponding images allow determination of the z position of a detected molecule, thereby enabling 3D reconstruction. To ensure reproducibility of results, the cylindrical lens is precisely positioned into the beam path as soon as the system is switched from 2D to 3D recording - not by hand, but automatically by a single mouse click through software control.

The Leica SR GSD 3D has been optimized in several ways for reliable localization results. All the optical components are apochromatically corrected to high Leica Microsystems standards.

Building on this, the software enables precise calibration of the system for the localization of molecules in x, y and z. Besides the standard calibration with gold beads, color-specific calibration with fluorescence beads or dye molecules of the specimen is available. The special SuMo technology (SUpressed MOtion) ensures minimum drift and maximum stability during detection.

“3D visualization of cellular structures with nanometer resolution allows scientists in biomedical research to gain new insights,” explains Sebastian Tille, Director Widefield Imaging at Leica Microsystems. “The new Leica SR GSD 3D is an excellent tool for gaining a better understanding of molecular structures or cellular protein transport processes, for example. Experts are already confirming the outstanding performance and reliability of the new system after first tests.”

The Leica SR GSD 3D is based on a fully automated TIRF system (Total Internal Reflection Fluo-rescence), allowing super-resolution to be combined with TIRF microscopy. The system can also be used for a wide variety of applications in all fields of live cell or advanced fluorescence microscopy.

As a multi-functional system, the Leica SR GSD 3D gives researchers the freedom to tailor the system exactly to their needs. Another key advantage of GSD technology is that it can be used with standard fluorescence labels.

The Leica SR GSD 3D is the successor to the Leica SR GSD, which was recognized by international expert commitees many times, such as with the prestigious R&D100 award.

Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More Than 5,200+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Leica, BMC in Munich Open Core Facility Bioimaging for Applied Cell Research
Leica Microsystems and the Biomedical Center (BMC) of the Ludwig-Maximilians-Universität (LMU) Munich, Germany, will inaugurate the new core facility Bioimaging.
Thursday, February 04, 2016
Markus Lusser is New President of Leica Microsystems
Appointment became effective July 1, 2015.
Wednesday, August 12, 2015
Yale University and Leica Microsystems Partner
Yale welcomes scientists to participate in core facility opening and super-resolution workshops October 20 through 31, 2014.
Tuesday, September 30, 2014
Leica Microsystems and Leica Biosystems Strengthen Market Position in Brazil
Long-standing Leica distributors Aotec have been acquired.
Tuesday, April 23, 2013
Leica Microsystems and Bausch + Lomb Sign Global Agreement
Bausch + Lomb to distribute Leica Microsystems’ medical products to ophthalmic customers in select global markets.
Friday, April 19, 2013
R&D 100 Award for Super-Resolution Microscope from Leica Microsystems
Leica SR GSD convinces jury with unprecedented performance in fluorescence microscopy.
Monday, August 06, 2012
Leica Microsystems Acquires Labindia’s Microscopy and Histopathology Divisions
High-tech market leader is set to boost growth with a local presence in India.
Thursday, November 17, 2011
License Agreement Between Leica Microsystems, the Max Planck Society and DKFZ
Developing the next Stimulated Emission Depletion microscopy generation.
Monday, November 14, 2011
Leica Announces new Chief Financial Officer
Martin Geisel has been announced as new CFO.
Monday, October 02, 2006
Scientific News
Unravelling the Role of Key Genes and DNA Methylation in Blood Cell Malignancies
Researchers from the University of Nebraska Medical Center have demonstrated the role of Dnmt3a in safeguarding normal haematopoiesis.
Salford Lung Study - The First Real World Clinical Trial
In this podcast, we learn about the Salford Lung Study and its potential to revolutionize the way we assess new drugs and treatments around the world.
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Editing Gene Mutations in Anemia
Researchers successfully use a new gene editing strategy to correct mutations that cause a form of anemia.
Genes Help Track Odd Migrations of Zika Mosquitoes
Study shows that mosquitoes carrying Zika virus or Dengue fever a genetically distinct around the world.
Nanomedicine Aims to Improve HIV Drug Therapies
New research aims to improve the administration and availability of drug therapies to HIV patients using nanotechnology.
Tumor Markers Reveal Lethality Of Bladder Cancers
Researchers found that detection of certain tumor cells in early stage cancers helps identify high-risk cancers.
Gene Editing Corrects Sickle Cell Mutation
Researchers demonstrate a potential pathway to developing gene-editing treatments for sickle cell disease.
Driving Mosquito Evolution to Fight Malaria
Researchers propose insect repellent in conjunction with insecticides to extend current insecticide lifetime.
Lab-on-a-Chip to Help Detect Cancer
In this podcast, we speak to Gustavo Stolovitsky to learn about his career and the work he is doing at IBM Research.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos