Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Researchers Identify Gene Involved in Response to Cocaine

Published: Monday, December 23, 2013
Last Updated: Sunday, December 22, 2013
Bookmark and Share
Researchers compared “J” and “N” strains of mice and used their differential responses to cocaine to identify the causative gene.

UT Southwestern neuroscience researchers have identified a gene that controls the response to cocaine by comparing closely related strains of mice often used to study addiction and behavior patterns.

The researchers suspect that the newly identified gene, Cyfip2, determines how mammals respond to cocaine, although it is too soon to tell what the indications are for humans or for addiction, said Dr. Joseph Takahashi, chair of neuroscience and a Howard Hughes Medical Institute investigator at UT Southwestern and the senior author of the study.

The findings, reported in Science, evolved from examining the genetic differences between two substrains of the standard C57BL/6 mouse strain: a “J” strain from the Jackson Laboratory (C57BL/6J) and an “N” strain from the National Institutes of Health (C57BL/6N). Researchers compared the two strains of mice and used their differential responses to cocaine to identify the causative gene.

“We found that the ‘N’ strain has accumulated mutations over time, one of which has a very strong effect on cocaine response,” Dr. Takahashi said. “We propose that CYFIP2 - the protein produced by the Cyfip2 gene - is a key regulator of cocaine response in mammals.”

The Takahashi laboratory has identified about 100 genetic differences that affect protein sequences between the two mouse strains, meaning that there are many genetic differences whose effects are not yet known, he added.

“We identified this gene by first using a forward genetics strategy to search for differences in traits between the two mouse strains. We found a difference in cocaine response between them, with the C57BL/6N strain showing a reduced behavioral response,” Dr. Takahashi said. “We then carried out genetic mapping and whole genome sequencing, which allowed us to pinpoint the Cyfip2 gene as the causative one in a rapid and unambiguous way.”

The C57BL/6J “J” mouse is the gold-standard strain for most research involving the mouse. For example, the reference sequence for the mouse genome, as well as most behavioral and physiological experiments, are based on the “J” strain. However, the International Knockout Mouse Consortium will be shifting emphasis to the “N” strain since they have created 17,000 embryonic stem cell lines with gene mutations that originate from the “N” strain. Thus, identifying genetic differences between these two mouse strains is important, Dr. Takahashi said.

“Although mouse geneticists pay close attention to the specific strains of mice that they use, it has not been generally appreciated that sublines of the same strain of mouse might differ so profoundly. Thus, a ‘C57BL/6’ mouse might appear to be the same, but in fact there are many, many sublines of this laboratory mouse, and it is important to know which exact one you are using. Since the knockout mouse project has produced so many mutations (17,000) derived from the ‘N’ strain, it will be even more important to keep in mind that not all C57BL/6 mice are the same.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More Than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

New Mechanism of Tuberculosis Infection
Researchers at UTSW Medical Center have identified a new way that tuberculosis bacteria get into the body, revealing a potential therapeutic angle to explore.
Friday, July 22, 2016
New Therapeutic Targets For Small Cell Lung Cancer Identified
Researchers at UTSW Medical Center have identified a protein termed ASCL1 that is essential to the development of small cell lung cancer.
Friday, July 22, 2016
New Mechanism of Tuberculosis Infection
Researchers have identified a new infection mechanism of tuberculosis that could lead to a new therapeutic angle.
Friday, July 22, 2016
New Method Detects Telomere Length for Research into Cancer, Aging
UT Southwestern Medical Center cell biologists have identified a new method for determining the length of telomeres, the endcaps of chromosomes, which can influence cancer progression and aging.
Friday, July 01, 2016
3-D Atomic Structure of Cholesterol Transporter
Researchers at UTSW have determined the 3-D atomic structure of a human sterol transporter that helps maintain cholesterol balance.
Tuesday, May 31, 2016
New $17 Million Cryo-Electron Microscope Center Provides Extraordinary Views
Institute has announced opening of a new $17 million cryo-EM facility housing a unique collection of instruments that researchers can use to view 3-D images of objects.
Thursday, May 12, 2016
New Autism Blood Biomarker Identified
Researchers at UT Southwestern Medical Center have identified a blood biomarker that may aid in earlier diagnosis of children with autism spectrum disorder, or ASD.
Friday, May 06, 2016
Enzyme Link Between Excessive Heart Muscle Growth, Cancer Growth
Researchers at UTSW have found that the drugs currently used to inhibit these enzymes in cancer may also be effective in treating enlargement of the heart muscle.
Saturday, April 16, 2016
Treatment of Common Prostate Cancer
Researchers at UTSW have found that the prostate cancer treatments suppress immune response and may promote relapse.
Friday, April 08, 2016
A Metabolic Twist that Drives Cancer Survival
A novel metabolic pathway that helps cancer cells thrive in conditions that are lethal to normal cells has been identified.
Friday, April 08, 2016
Novel Metabolic Twist that Drives Cancer Survival
Researchers at CRI at UT Southwestern have identified a novel metabolic pathway that helps cancer cells thrive in conditions that are lethal to normal cells.
Thursday, April 07, 2016
Structure of Crucial Enzyme Identified
Researchers at UTSW have determined the atomic structure of an enzyme that plays an essential role in cell division and better treatment of cancer.
Thursday, March 31, 2016
Mutation That Causes Rare Disease
A mutation has been discovered that causes a rare systemic disorder known as XLPDR and confirmed a role for nucleic acids in immune function.
Tuesday, March 29, 2016
Promoting Liver Tissue Regeneration
Researchers at CRI have reported that inactivating a certain protein-coding gene promotes liver tissue regeneration in mammals.
Saturday, March 26, 2016
Lupus Study Shows Precision Medicine’s Potential to Define the Genetics of Autoimmune Disease
Researchers at UT Southwestern have used next-generation DNA sequencing technology to identify more than 1,000 gene variants that affect susceptibility to SLE.
Saturday, March 19, 2016
Scientific News
Breakthrough Flu Vaccine Inhibited by Pre-existing Antibodies
Universal truths – how existing antibodies are sabotaging the most promising new human flu vaccines.
Researchers Develop Software That Could Facilitate Drug Development
AptaTRACE can identify aptamers, potentially speed drug advancement.
Gene Therapy for Metabolic Liver Diseases
Researchers have tested gene therapy in pigs from hereditary tyrosinemia type 1, with corrected liver cells being transplanted into the diseased liver.
Zika Vaccine Candidates Show Promise
Two experimental vaccines have shown promise against a major viral strain responsible for the Brazilian Zika outbreak.
New Medication Shows Promise Against Liver Fibrosis in Animal Studies
Liver fibrosis is a gradual scarring of the liver that puts people at risk for progressive liver disease and liver failure.
Raw Eggs Deemed Safe to Eat
A report published today by the Advisory Committee on the Microbiological Safety of Food (ACMSF) into egg safety has shown a major reduction in the risk from salmonella in UK eggs.
Monitoring TTX Toxin in Shellfish
In a number of small studies, mussels and oysters from the eastern and northern part of the Oosterschelde in Holland were found to contain tetrodotoxin (TTX).
Gene Terapy for Muscle Wasting Developed
New gene therapy could save millions of people suffering from muscle wasting disease.
NIH Begins Yellow Fever Vaccine Trial
NIH has initiated an early-stage clinical trial of a vaccine to protect against yellow fever.
Gene-Editing 'Toolbox' Targets Multiple Genes Simultaneously
Researchers have designed a system that modifies, or edits, multiple genes in a genome at once while minimising unintentional effects.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!