TATAA Biocenter and MultiD Analyses have released GenEx version 6 for accurate analysis of qPCR data compliant with current Clinical Laboratory Standards Institute (CLSI) guidelines.
The new analyses in GenEx 6 include: Estimating PCR efficiency, testing for outliers, testing linear model, estimating dynamic range, estimating random error, estimating limit of detection, estimating limit of quantification, estimating concentrations of unknowns, evaluation of precision, and verification of precision.
For all estimates confidence intervals are calculated. Additional new features include: transcript distribution for single cell analysis, Survival Analysis, Receiver Operator Characteristics (ROC), Wizard for ProSeek protein analysis, and reader for 3DGene microRNA analysis.
“I have been using GenEx for all my qPCR analyses for the past 5 years. With GenEx I can be sure that my data normalization and analyses will be MIQE compliant. Whether I’m analyzing qPCR arrays, data with a standard curve or determining a relative difference, it takes little time to import the files I need and obtain my final data set” says Greg Shipley, President of Shipley Consulting, LLC.
“We have been using GenEx for many years at Technical University of Munich. With every new released version we are impressed by the many clever functionalities introduced for RT-qPCR analysis, especially the data normalization and visualization tools like PCA or HCA”, says Michael W. Pfaffl.
Pfaffl continued, “The new CLSI compliant analyses are most interesting to us and a natural extension of the MIQE guidelines”.