Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Studying Metal-organic Frameworks Using Second Harmonic Generation Microscopy

Published: Tuesday, June 10, 2014
Last Updated: Tuesday, June 10, 2014
Bookmark and Share
Linkam reports on how the THMS600 temperature stage is being used to study metal-organic frameworks.

Linkam Scientific Instruments’ THMS600 temperature controlled stage is being used at the University of Leuven in the study of metal-organic frameworks using Second Harmonic Generation Microscopy.

The techniques most commonly used in microscopy have not changed much since its invention in the 16th century. But with the advancement of technology and the demand for more in-depth information, newer techniques such as Second Harmonic Generation (SHG) Microscopy are being used by scientists worldwide to further their research.

Dr Karen Markey from the University of Leuven, Belgium, is one such researcher who is reaping the benefits of these advancements by using SHG Microscopy to investigate the structure of materials in a manner that cannot be achieved through other, more conventional microscopy techniques.

Predominantly, SHG microscopy is used in the biological field because this technique allows for the imaging of samples, with non-centrosymmetric molecular structures, using contrast produced from the sample itself therefore negating the need to use dyes and colours which can damage living tissue. SHG can also achieve high resolution visualization and even Three Dimensional imaging of the sample without needing to excite the molecules run the risk of it being subjected to photo toxicity - as can happen in techniques such as fluorescence microscopy.

Dr Markey and her colleagues in the university’s molecular electronics and photonics department have been using and fine tuning an SHG technique developed by Assistant Professor Monique van der Veen from the Technical University of Delft that can allow them to study the structure of metal-organic frameworks (MOFs). As part of this set-up, they have generally been using a wide field microscope and, more recently, a laser scanning microscope with an intense frequency of 800 nm. The team take several images of the same sample, but use different polarizations of the laser light. Plotting the variation of SHG intensity as a function of the polarization gives an insight into the crystal structure of the samples.

With such small variations in optical density being of such importance, the control of the environmental conditions in which the sample is being held becomes even more vital. This was possible using a Linkam THMS600 stage.

Dr Markey was particularly impressed by the variety of parameters that can be controlled by the stages, she said:”You can easily set the desired temperature programs and also measure under N2 flow, which is important for the materials we work with. It is an ‘all in one’ solution.”

As these optical techniques evolve with time, more and more researchers in various fields will find a way to adapt them to their studies and therefore help paint a much clearer picture about the microscopic world.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Linkam and Cambridge IVF Collaborate to Develop New Protocols for Sperm Testing
The two companies are working together to develop new instrumentation and protocols to improve the reliability of sperm testing.
Monday, May 18, 2015
Advancing the Knowledge of Pharmaceutical Processes
Linkam Scientific Instruments report on the use of their stages in the study of pharmaceutical processes.
Wednesday, February 26, 2014
Imaging Fat Crystals in Chocolate
Scientists from Loughborough and Nottingham Universities have used the Linkam Linksys32-DV software to visualise and measure the in-situ growth of fat crystals on the surface of chocolate.
Tuesday, October 09, 2012
Understanding Protein Crystallization Growth at the University of Leeds Using a Temperature Stage
LTS350 hot-stage system used to visualize and grow HEW lysozyme crystals.
Wednesday, June 27, 2012
Temperature Controlled Stage Used in Crystallisation Processes
Linkam Scientific Instrument report on the work of Professor Yves Henri Geerts from the Université Libre de Bruxelles where he uses a specially designed temperature stage to study crystallisation processes in opto-electronic thin films.
Friday, December 09, 2011
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
World’s First Therapeutic Venom Database
Open-source library describes nearly 43,000 effects on the human body.
Biologists Induce Flatworms to Grow Heads and Brains of Other Species
Findings shed light on role of a new kind of epigenetic signaling in evolution, could yield clues for understanding birth defects and regeneration.
Fat Cells Originating from Bone Marrow Found in Humans
Cells could contribute to diabetes, heart disease.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos