Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Automating Deep Characterization of Biotherapeutic Proteins

Published: Thursday, June 19, 2014
Last Updated: Tuesday, July 01, 2014
Bookmark and Share
Thermo's new PepFinder software streamlines relative and absolute quantitation, and identification of proteins from biological samples using LC-MS.

As protein-based drugs become more important in treating major diseases, producers face the critical challenge of deeply characterizing these highly-complex molecules for efficacy and safety, as well as to meet quality requirements. Now there’s a new software tool designed to increase the speed, quality and confidence in comprehensive biotherapeutic protein characterization useful for drug development and production quality control.

Thermo Scientific PepFinder software is designed to streamline relative and absolute quantitation, and identification of proteins from biological samples using liquid chromatography-mass spectrometry.

Therapeutic proteins such as monoclonal antibodies, interferons, and insulin must be produced in biologically-active forms that  have proper folding and have specific post-translational modificiations (PTM). Traditional analysis of these complex biological molecules is highly complex and time-consuming. PepFinder software uses multidimensional dynamic search capabilities to automatically integrate complex data into concise reports.

“This is a new paradigm for peptide mapping data analysis,” said Ken Miller, vice president, marketing mass spectrometry for Thermo Fisher Scientific. “Customers can expect to reduce analysis time from weeks to hours in some cases, while generating high-confidence, comprehensive characterization reports, including known and unknown modifications.”

“Using PepFinder, a complex protein was analyzed with great details (including PTMs), and with great ease and speed!” said Shiaw-Lin (Billy) Wu, Head of Analytical Sciences at BioAnalytix and Research Associate Professor at Northeastern University.

PepFinder software, based on Mass Analyzer software licensed from Amgen, is compatible with Thermo Scientific LC-MS instruments, including the industry-leading high resolution, accurate-mass Orbitrap-based systems. This software is designed for fast, high-quality PTM profiles and peptide identifications based on a novel prediction algorithm. The software displays sequence coverage maps, automates relative changes in site specific PTMs for rapid monitoring of modifications in a single sample or across sample comparison. The software is written to provide an automated workflow for glycopeptides ID, disulfide bond mapping and other common PTM’s, including oxidation, deamidation and phosphorylation.  This can eliminate time consuming and error-prone manual PTM mapping.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Advancing Lithium-Ion Battery Research
The increasing number of components in a lithium-ion battery makes it essential to use a variety of analytical methods to attain complete characterization.
Wednesday, October 28, 2015
Thermo Scientific Chromatography Columns/Consumables Catalog Goes Mobile
2014-15 Chromatography Columns and Consumables Catalog now available in print, pdf and interactive mobile versions.
Thursday, February 27, 2014
Thermo Collaborates with Leading EU and UK Pesticide Testing Labs
The company's Pesticide Analysis Center of Excellence provides technology, consultation and support.
Tuesday, November 19, 2013
Thermo Scientific at ASMS 2013
Learn how scientists and researchers are transforming science by applying innovative solutions to a wide range of qualitative and quantitative applications.
Monday, May 13, 2013
Thermo Fisher Scientific Reports First Quarter 2013 Results
Adjusted earnings per share grew 17% to a record $1.37 with revenue increased 4% to a first quarter record of $3.19 billion.
Thursday, April 25, 2013
Thermo Fisher Scientific to Acquire Life Technologies
Transaction expected to create an unrivaled leader in serving research, specialty diagnostics and applied markets.
Wednesday, April 17, 2013
Thermo Fisher Scientific to Acquire Life Technologies Corporation
The definitive agreement will see Thermo Fisher will acquire Life Technologies for $76.00 in cash per fully diluted common share or approximately $13.6 billion.
Monday, April 15, 2013
Thermo Fisher Scientific Facilities Achieve ISO 13485 Certification
The facilities in San Jose, CA and Franklin, MA have achieved the internationally recognized standard of quality management for the design and manufacture of medical devices.
Thursday, April 04, 2013
Thermo Fisher Scientific to Acquire picoSpin
Thermo Fisher Scientific Inc. has completed a purchase of picoSpin, LLC, creators of the world’s first miniature NMR spectrometer.
Friday, December 21, 2012
Hyperion Succesfully Completes Laboratory Infrastructure Project for Hellenic Petroleum
Hyperion and the Hellenic Petroleum Group have completed a major upgrade and extension project for the LIMS at three Greek petroleum refineries of the Group.
Wednesday, November 21, 2012
Thermo Fisher Scientific Reports Q3 Revenue and EPS Results
Revenue increased 5% to a record $3.09 billion for the third quarter with adjusted operating income rising 5% to $576 million.
Wednesday, October 24, 2012
Thermo Fisher Scientific and Immundiagnostik Collaborate
The collaboration aims to develop novel mass spectrometry-based assays for qualitative and quantitative analysis of proteins and peptides.
Wednesday, October 17, 2012
Thermo Fisher Scientific and bioMérieux Renew Partnership
The companies have renewed their non-exclusive, long-term, royalty-bearing agreement for Procalcitonin testing.
Wednesday, October 03, 2012
Seminars on Advanced Solutions for Nutraceutical Analysis
Seminar sessions will discuss Company’s chromatography and elemental analysis techniques to optimize nutraceutical processes.
Tuesday, September 25, 2012
Thermo Scientific Opens Molecular Biology Center of Excellence in Lithuania
New facility expands R&D and manufacturing capabilities in eastern Europe for molecular, protein and cellular biology products.
Friday, September 21, 2012
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos