Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Revealing the Role of “Precocious” Dendritic Cells in Inflammatory Response

Published: Monday, July 07, 2014
Last Updated: Monday, July 07, 2014
Bookmark and Share
Discovery makes it possible for researchers to explore how these “precocious” cells escalate the body’s immune response when the body is under attack.

In work published in Nature, scientists at the Broad Institute and Fluidigm Corporation used the C1TM Single-Cell Auto Prep System to prepare cells for single-cell sequencing - enabling the discovery of a subpopulation of “precocious” cells expressing antiviral genes earlier than the majority of dendritic cells exposed to the same antigen. Normally, the ability to detect the actions of these  precocious expresser” cells would be lost due to averaging across many millions of cells in bulk cell studies. 

The Nature paper also highlighted the role of cellular heterogeneity and its importance in cell-to-cell  communication during an immune response. In typical studies, cells are studied together in one environment, allowing the cells to communicate and signal to one another. However, the unique properties of Fluidigm’s C1 Single-Cell Auto Prep System allowed the researchers to isolate the cells from one another to identify the specific cells expressing antiviral genes. Because these cells were isolated, the non-expressing cells did not surmount a response, indicating that cell-to-cell communication plays a critical role in promoting immune response.

In addition to the discovery of a rare subpopulation of cells, this research represented a new benchmark for sheer volume of single-cell data. The 1,774 cells represented in this paper provide one of the largest single-cell genomic datasets published to date and one of the largest mRNA sequencing datasets available anywhere.

In order to identify and characterize these rare “precocious” cells, which make up only 1% of dendritic cell populations, researchers needed to scale up to a high number of cells. To do this, they first needed to solve two key problems: how to prepare single-cell libraries for thousands of cells, and how to sequence such large sample numbers affordably.

To prepare the huge number of single-cell libraries, the researchers relied on the C1 Single-Cell Auto Prep System, which had just been introduced to the marketplace at the time these experiments began. The C1 system’s microfluidic technology enabled the researchers to rapidly and reliably isolate, process, and profile individual cells for genomic analysis. During the study, the group discovered that they could achieve stable data with single cells at less than one million reads (compared to run rates of at least 5 to 10 million reads when conducting bulk mRNA sequencing). The ability to obtain reliable data faster greatly reduced the cost of running the experiment.

The paper, "Single-cell RNA-seq reveals dynamic paracrine control of cellular variation" can be accessed below.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Fluidigm, OpGen Collaborate
Fluidigm and OpGen have announced an agreement to collaborate on instruments and kits to test for multi-drug resistance genes in bacteria, fungi, and viruses.
Tuesday, July 14, 2015
Early Access Program for Fluidigm's New Imaging Mass Cytometry Platform
Prototypes of the product are expected to begin shipping to early access partners by the end of this year.
Sunday, August 03, 2014
Fluidigm to Acquire DVS Sciences
The acquisition consideration of approximately $207.5 million consists of a combination of Fluidigm common stock and cash.
Wednesday, January 29, 2014
Fluidigm Reports Strong Q1 2013 Results
Revenue up 33% and margins increase 300 basis points compared to Q1 2012.
Friday, May 03, 2013
Fluidigm to Develop and Supply a Custom High-throughput Prep System for HLA Typing
Custom system will ultimately enable DKMS Life Science Lab to type several thousand samples per week.
Friday, May 03, 2013
Singapore Single-Cell Research Centre Opens Door for Asian Biological Discoveries
Centre dedicated to diagnosis and treatment derived from single-cell exploration.
Monday, April 29, 2013
Fluidigm Announces $60 Million Public Offering of Common Stock
Company has closed the previously announced underwritten public offering of 4,209,000 shares of its common stock at a price to the public of $14.25 per share for gross proceeds of approximately $60 million.
Wednesday, August 22, 2012
Fluidigm Announces Unrestricted Sales into Prenatal Health
Fluidigm collaboration ends.
Tuesday, June 19, 2012
New Research Center to Focus on Mammalian Single Cell Genomics
New Center will feature a complete suite of Fluidigm single-cell tools, protocols and technologies.
Friday, June 15, 2012
Fluidigm Reports Financial Results for First Quarter 2012
Q1 total revenue up 26% compared to Q1 2011.
Monday, May 14, 2012
Fluidigm China Opens Its Doors
Company expects to rapidly build strong presence in country and improve services to Chinese customer base.
Monday, April 23, 2012
Fluidigm and AH Diagnostics Sign Distribution Deal for Scandinavia
AH diagnostics will offer Fluidigm's complete portfolio, including the complete line up of Dynamic Array(TM), Digital Array(TM) and Access Array IFCs for real-time PCR, digital PCR, single-cell gene expression, sample preparation for next-generation sequencing, and SNP genotyping.
Tuesday, January 31, 2012
Fluidigm and Life Technologies Cross-License a Broad Set of Intellectual Properties
Fluidigm Corporation announces that it has entered into a set of cross-licensing and sub-licensing agreements on a range of intellectual properties with Life Technologies.
Tuesday, July 12, 2011
Fluidigm Reports Financial Results for First Quarter 2011
Total revenue for the first quarter of 2011 was $8.7 million, an increase of 30% from $6.7 million in the first quarter of 2010.
Wednesday, May 11, 2011
Fluidigm Announce Israeli Distribution Deal
Fluidigm has appointed Eisenberg Brothers as distributor in Israel and Eisenberg Brothers offers Fluidigm's complete portfolio of BioMark™, EP1™, and Access Array™ systems.
Tuesday, March 01, 2011
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!