Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Wisconsin Scientists Find Genetic Recipe to Turn Stem Cells to Blood

Published: Monday, July 14, 2014
Last Updated: Tuesday, July 15, 2014
Bookmark and Share
The ability to reliably and safely make in the laboratory all of the different types of cells in human blood is one key step closer to reality.

Writing today in the journal Nature Communications, a group led by University of Wisconsin-Madison stem cell researcher Igor Slukvin reports the discovery of two genetic programs responsible for taking blank-slate stem cells and turning them into both red and the array of white cells that make up human blood.

The research is important because it identifies how nature itself makes blood products at the earliest stages of development. The discovery gives scientists the tools to make the cells themselves, investigate how blood cells develop and produce clinically relevant blood products.

“This is the first demonstration of the production of different kinds of cells from human pluripotent stem cells using transcription factors,” explains Slukvin, referencing the proteins that bind to DNA and control the flow of genetic information, which ultimately determines the developmental fate of undifferentiated stem cells.

During development, blood cells emerge in the aorta, a major blood vessel in the embryo. There, blood cells, including hematopoietic stem cells, are generated by budding from a unique population of what scientists call hemogenic endothelial cells. The new report identifies two distinct groups of transcription factors that can directly convert human stem cells into the hemogenic endothelial cells, which subsequently develop into various types of blood cells.

The factors identified by Slukvin’s group were capable of making the range of human blood cells, including white blood cells, red blood cells and megakaryocytes, commonly used blood products.

 “By overexpressing just two transcription factors, we can, in the laboratory dish, reproduce the sequence of events we see in the embryo” where blood is made, says Slukvin of the Department of Pathology and Laboratory Medicine in the UW School of Medicine and Public Health and the Wisconsin National Primate Research Center.

The method developed by Slukvin’s group was shown to produce blood cells in abundance. For every million stem cells, the researchers were able to produce 30 million blood cells.

A critical aspect of the work is the use of modified messenger RNA to direct stem cells toward particular developmental fates. The new approach makes it possible to induce cells without introducing any genetic artifacts. By co-opting nature’s method of making cells and avoiding all potential genetic artifacts, cells for therapy can be made safer.

“You can do it without a virus, and genome integrity is not affected,” Slukvin notes.

Moreover, while the new work shows that blood can be made by manipulating genetic mechanisms, the approach is likely to be true as well for making other types of cells with therapeutic potential, including cells of the pancreas and heart.

An unfulfilled aspiration, says Slukvin, is to make hematopoietic stem cells, multipotent stem cells found in bone marrow. Hematopoietic stem cells are used to treat some cancers, including leukemia and multiple myeloma. Devising a method for producing them in the lab remains a significant challenge.

“We still don’t know how to do that,” Slukvin notes, “but our new approach to making blood cells will give us an opportunity to model their development in a dish and identify novel hematopoietic stem cell factors.”




Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Antibody Targets Key Cancer Marker
University of Wisconsin-Madison researchers have created a molecular structure that attaches to a molecule on highly aggressive brain cancer and causes tumors to light up in a scanning machine.
Tuesday, November 10, 2015
Petri Dish Tumor Test
In a highly successful, first-of-its-kind endeavour, researchers have created a "tumor in a dish:" an ex vivo microenvironment that can accurately anticipate a multiple myeloma patient's response to a drug.
Monday, June 15, 2015
3D for Top-Down Proteomics: Extra Dimension for More Proteins
Researchers from the University of Wisconsin- Madison have tested the possible applications of adding a "third dimension" to chromatography.
Tuesday, May 26, 2015
New Process Transforms Wood, Crop Waste into Valuable Chemicals
Scientists disclosed a new method to convert lignin, a biomass waste product, into simple chemicals.
Thursday, November 06, 2014
In Directing Stem Cells, Context Matters
The surface cells are grown on has a profound effect on differentiation.
Tuesday, September 09, 2014
New Gene Repair Technique Promises Advances in Regenerative Medicine
Using human iPSC’s and DNA-cutting protein from meningitis bacteria, researchers have created an efficient way to target and repair defective genes.
Thursday, August 15, 2013
Adult Cells Transformed into Early-Stage Nerve Cells, Bypassing the Pluripotent Stem Cell Stage
A UW-Madison research group has converted skin cells from people and monkeys into a cell that can form a wide variety of nervous-system cells.
Tuesday, May 07, 2013
Cells from Skin Create Model of Blinding Eye Disease
For the first time, Wisconsin researchers have taken skin from patients and, using induced pluripotent stem cell (iPSC) technology, turned them into a laboratory model for an inherited type of macular degeneration.
Monday, November 12, 2012
At Smallest Scale, Liquid Crystal Behaviour Portends New Materials
Latest research has shown that liquid crystals may have some new technological tricks in store.
Saturday, May 05, 2012
Stem Cell Symposium to Address Brain, Nervous System
Seventh Symposium will focus on the mechanisms of neural development, modeling neural disorders, and harnessing the potential of neural regeneration.
Thursday, April 12, 2012
Mouse Genome Sequences Reveal Variability, Complex Evolutionary History
Genomes of 17 different strains of mice will aid studies ranging from human disease to evolution.
Saturday, September 17, 2011
Study Reveals Critical Similarity Between Two Types of Do-it-All Stem Cells
Researchers at the University of Wisconsin-Madison report the first full measurement of the proteins made by both types of stem cells.
Monday, September 12, 2011
Researchers Solve Membrane Protein Mystery
UW-Madison researchers solves 25-year mystery that may lead to better treatments for people with learning deficits and mental retardation.
Friday, July 29, 2011
UW-Madison Scientists Played Role in Potato Genome Project
Scientists are part of an international consortium that has successfully sequenced and analyzed the potato genome.
Monday, July 11, 2011
Study Shows Patient's Own Cells May Hold Therapeutic Promise After Reprogramming, Gene Correction
Scientists from the Morgridge Institute for Research, the University of Wisconsin-Madison, the University of California and the WiCell Research Institute moved gene therapy one step closer to reality.
Tuesday, April 26, 2011
Scientific News
Open Source Seed Initiative – A Welcome Boost to Global Crop Breeding
A team of plant breeders, farmers, non-profit agencies, seed advocates, and policymakers have created the Open Source Seed Initiative.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
A New Way Out for Stem Cells
Researchers at North Carolina State University have discovered that therapeutic stem cells exit the bloodstream in a different manner than was previously thought.
One Giant Leap for the Future of Safe Drug Delivery
Sheffield engineers make major breakthrough in developing silk ‘micro-rockets’ that can be used safely in biological environments.
Designing Potential AIDS Vaccine Candidates
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anticancer Drug Stops Ebola Virus Molecule in its Tracks
A team of scientists from the University of Oxford have successfully mapped the structure of the Ebola virus molecule that drives the attack strategy and leads to fatal infections in humans.
Assessing the Effectiveness of Genome-Editing Technologies
Researchers have developed a cost-effective and rapid method for assessing edits generated by CRISPR-Cas9 and other genome-editing technologies.
Anthrax Proteins Might Help Treat Cancerous Tumors
Studies in mice reveal novel treatment regimen.
New Cancer Drug Target Found in Dual-Function Protein
Findings from a study from TSRI have shown that targeting a protein called GlyRS might help to halt cancer growth.
Key to Chronic Fatigue Syndrome is in Your Gut, Not Head
Researchers report they have identified biological markers of the disease in gut bacteria and inflammatory microbial agents in the blood.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!