Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

ChIP Sequencing Kit for Next Generation Sequencing

Published: Tuesday, July 22, 2014
Last Updated: Tuesday, July 22, 2014
Bookmark and Share
The assay kit from Porvair Sciences extends the benefits of their proprietary Chromatrap® solid state ChIP technology.

Porvair Sciences has developed a new ChIP sequencing assay kit extending the benefits of their proprietary Chromatrap® solid state ChIP technology to preparation of purified Chromatin for the generation of sequencing libraries.

Chromatin immunoprecipitation (ChIP) is a technique used to detect interactions between proteins and DNA, and is based on the enrichment of DNA associated with a specific protein of interest. Combining ChIP with massively parallel sequencing (ChIP-seq) allows the accurate survey of interactions between proteins and DNA, enabling the study of epigenetic marks which is essential for a full understanding of transcriptional regulation of genes. A precise and detailed map of protein interactions with DNA is vital for interpreting regulatory networks that underlie many biological processes and disease states, including cancer. Recent advances have identified that that the interplay between chromatin and transcription is much more dynamic and complex than initially anticipated. Previous technology using microarrays were limited by the number of unique species-specific oligonucleotide probes that could be hybridised to the chip surface. Next generation sequencing has the ability to sequence tens or hundreds of millions of short DNA fragments simultaneously, without being hybridised to an array. With single-base pair resolution, fewer artefacts, greater coverage and a larger dynamic range, ChIP-seq offers significant advantages over past technology and experiments which could only be imagined a few years ago are now becoming a reality.

Chromatrap® is a new quicker, easier and more efficient way of performing ChIP-seq assays (Patent No. GB2482209). It uses discs of an inert, porous polymer to which Protein A or Protein G has been covalently attached to maximise the capture efficiency of the target chromatin/antibody complex. Chromatrap utilises the solid state technology in parallel with high throughput sequencing to deliver a precise ChIP-seq protocol from small cell numbers and chromatin concentrations. Specifically adapted for greater chromatin concentrations, Chromatrap ChIP-seq now combines the dynamic range of Chromatrap with the downstream analysis power of deep sequencing, allowing the genome wide identification of transcription factor binding sites and specific DNA associated protein modifications with no limitation in scale and resolution.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Porvair Filtration Group Receives Queen’s Award for Enterprise in International Trade
This award had been made in recognition of Porvair’s substantial growth in overseas business, achieving increased year-on-year growth in sales of exported products over the past three years.
Tuesday, April 21, 2015
Study Investigates Inherent Contamination in Deep Well Microplates
Study gives data on microplates from numerous manufacturers based in Europe, USA and China.
Tuesday, October 21, 2014
Reference Guide to Microplate Chemical Compatibility
Porvair’s new version of chemical compatibility chart.
Wednesday, August 14, 2013
Biomedical Researcher wins Microplate Catalogue Prize Draw
A scientist at the Genomics CoreLab of the NIHR Biomedical Research Centre at Addenbrooke's Hospital (Cambridge, UK) has won Porvair Sciences' latest free prize draw for people registering online for the company's 2010-2011 Microplate Catalogue.
Wednesday, January 26, 2011
Porvair Filters Ensure Quality of Polymer Melt Process
The Company has announced a major order worth in excess of £1 million to supply leaf disc filters to Lindauer Dornier GmbH.
Wednesday, September 29, 2010
Porvair Sciences Launches www.microplates.com
New website designed to offer a wide variety of useful information about microplates or microplate technologies.
Thursday, July 16, 2009
Porvair Sciences Invests in new Microplate Production Facility
Porvair Sciences has announced the opening of a new Class 10000 cleanroom at their Wrexham, UK facility.
Tuesday, October 09, 2007
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!