The new illustra Single Cell GenomiPhi DNA amplification kit from GE Healthcare Life Sciences provides a simple method for producing amplified genomic DNA reliably and reproducibly.
Delivering higher genome coverage with fewer mutations than PCR-based whole genome amplification methods, Single Cell GenomiPhi enables a more accurate representation of the original genome, giving researchers a tool that delivers increased confidence in results.
The amount of genomic DNA needed to carry out a full range of testing can represent significant challenges across numerous areas such as cancer, and environmental research. illustra Single Cell GenomiPhi has been optimized to wholly amplify genomic DNA from as little as a single cell, generating micrograms of high quality DNA for use in downstream applications.
Lysis reagents are optimized to fully release genomic DNA from the cell and subsequently denature the DNA to enable optimal amplification and coverage. Background amplification, often associated with multiple displacement amplification (MDA), is suppressed throughout the incubation so that only input DNA is amplified.
High quality reagents and an optional, proprietary enzymatic clean-up step in the protocol ensure that any potential DNA contaminants introduced during set-up are removed before each individual reaction.
In addition, manufacturing processes, including UV and enzymatic reagent cleanup, help to ensure that all kits are free from any detectable DNA contamination and enable sensitivity of amplification down to 1 fg of gDNA.