Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

ARTEL Study Shows that Pipetting Warm and Cold Liquids Causes Error of up to 37 Percent

Published: Tuesday, July 17, 2007
Last Updated: Thursday, July 26, 2007
Bookmark and Share
Data taken during Mission #2 of the ARTEL Extreme Pipetting Expedition shows inaccuracy by 37 percent when handling fluids at temperatures different from the pipettes.

Data taken at Yellowstone National Park during Mission #2 of the ARTEL Extreme Pipetting Expedition show that pipettes deliver volumes inaccurate by up to 37 percent when handling fluids at temperatures different from the pipettes.

Called thermal disequilibrium, this source of error is prevalent in today’s laboratories, as many assays and tests require the addition of liquids which are warmer or colder than the laboratory environment and the actual pipettes.

Yellowstone was selected for Mission #2 of the Expedition because it is emblematic of thermal variation and disequilibrium. The Extreme Pipetting Expedition is a multi-phase, year-long scientific study to investigate and illustrate the impact of laboratory conditions on data integrity.

ARTEL is releasing the results from Mission #2 of the Extreme Pipetting Expedition at the 2007 AACC Annual Meeting & Clinical Lab Expo, San Diego, CA, July 17-19, Booth #4143. The data will also be published at www.artel-usa.com/extreme.

“From our testing at Yellowstone, it is clear that pipettes show a bias in volume delivery when dispensing fluids at different temperatures, and the error is especially significant when handling small liquid quantities,” says George Rodrigues, Ph.D., Senior Scientific Manager at ARTEL and Expedition Leader. “Laboratories must account for this variation to ensure good accuracy and precision in test results.”

Data taken at Yellowstone show that error caused by thermal disequilibrium was significant when using smaller volume pipettes at their minimum volumes. For example, a two-microliter variable-volume pipette set to deliver 0.2 microliters over-delivered cold liquid (3oC) by 37 percent and under-delivered warm liquid (45oC) by 24 percent, compared with liquid at room temperature (21oC). When set to deliver its maximum volume (two microliters), the pipette over-delivered cold liquid by one percent and under-delivered warm liquid by seven percent.

As expected, error was present, but smaller, when working with larger liquid volumes. The 20-microliter pipette set to deliver two microliters over-delivered cold liquid by four percent. When the pipette was set to deliver its maximum volume of 20 microliters, the overdelivery amounted to 0.6 percent.

“There are not many laboratory managers who would knowingly accept error of more than a few percent, let alone error of 37 percent,” says Doreen Rumery, Quality Control Manager at ARTEL and Extreme Pipetting Expedition member. “Yet many technicians frequently pipette warm and cold liquids in critical testing and assay work without accounting for volume variation.”

The aim of the Extreme Pipetting Expedition is to raise awareness about common laboratory extremes that can affect data quality, including the thermal disequilibrium phenomenon.

“Since the magnitude of error is dependent on a number of protocol-specific details, such as pipetting speed and type of sample container, a one-size-fits-all correction factor does not currently exist,” says Rodrigues. “ARTEL will continue to study thermal disequilibrium to help laboratories develop strategies to minimize this source of laboratory error.”

Volumes were measured using the ARTEL PCS® (Pipette Calibration System), a portable measurement technology based on ratiometric photometry.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

International Standards Group Issues Call for Liquid Handling Experts
Artel to host September meeting of global experts in Maine.
Wednesday, July 01, 2015
Artel Acquires Software to Expand its Liquid Handler Performance Verification Capabilities
Artel acquires exclusive rights to the LabGauge product line from Louisville, Kentucky-based StackWave.
Monday, January 27, 2014
Artel Grows Technical Services Team for Liquid Handling Quality Assurance
To support the expanded application of Artel technology and services.
Monday, February 13, 2012
Artel to Lead Workshop on Best Practices in Automated Liquid Handling
Hands-on course speeds up learning process by providing immediate and quantified experiment results.
Thursday, September 23, 2010
Artel Launches First Interactive Web Portal Dedicated to Liquid Handling
Liquid Handling Talk serves as information resource for pipetting and automated liquid handling.
Thursday, January 29, 2009
ARTEL Launches New Website Designed as Information Portal on Liquid Handling Quality Assurance
Features content covering topics from pipette calibration to performance verification of automated liquid handlers and proper pipetting technique.
Friday, April 04, 2008
ARTEL Extreme Pipetting Expedition to Visit Death Valley to Test Impact of Dry Heat on Laboratory Data
At Death Valley, ARTEL will test how dry and hot environmental conditions alter pipetted volumes.
Friday, September 07, 2007
ARTEL Holds Industry’s First Multichannel Pipetting Technique Certification Seminar
To help laboratories improve pipetting technique and reduce laboratory error, ARTEL held a Pipetting Proficiency Training and Certification Seminar.
Wednesday, July 11, 2007
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Immunotherapy Agent Benefits Patients with Drug-Resistant Multiple Myeloma in First Human Trial
Daratumumab proved generally safe in patients, even at the highest doses.
Low-level Arsenic Exposure Before Birth Associated with Early Puberty in Female Mice
Study examine whether low-dose arsenic exposure could have similar health outcomes in humans.
Inciting an Immune Attack On Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
‘Mutation-Tracking’ Blood Test for Breast Cancer
Scientists have developed a blood test for breast cancer able to identify which patients will suffer a relapse after treatment, months before tumours are visible on hospital scans.
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Intensity of Desert Storms May Affect Ocean Phytoplankton
MIT study finds phytoplankton are extremely sensitive to changing levels of desert dust.
Common ‘Heart Attack’ Blood Test May Predict Future Hypertension
Small rises in troponin levels may have value as markers for subclinical heart damage and high blood pressure.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!