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TATAA Biocenter Scientists Pioneer 3-Way Expression Profiling

Published: Friday, April 25, 2008
Last Updated: Friday, April 25, 2008
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A TATAA Biocenter researcher has performed a 3-way expression profiling study of the metabolism of Saccharomyces cerevisiae also called baker’s yeast.

Taking real-time PCR one step forward researcher at TATAA Biocenter has performed a 3-way expression profiling study of the metabolism of Saccharomyces cerevisiae (baker’s yeast).

While regular (2-way) expression profiling, where a set of samples are analyzed for many genes, is challenging to most researchers, the TATAA scientists analyzed the expression of many (18) genes at 8 different time points after addition of glucose to yeast grown in ethanol of 4 different strains. The experimental design generates a cube of data.

The company said that using GenEx software the data were analyzed using augmented principal component analysis, which revealed important co-regulations between the metabolic genes, including their sensitivities to genetic modifications. This novel 3-way approach to study expression pathways is expected to become very important in studies of potential drug targets, biological responses, and genetically modified species.

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