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Thursday, July 10, 2014
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Use of Nextgen to Study Circulating Biom...Next Generation Crop Genomics...MagSi-NGSPREP - NGS Library Preparation ...

Featured Products
Featured ProjectSX-8G IP-Star® Compact Automated System
SX-8G IP-Star® Compact Automated System

Webcasts
Use of Nextgen to Study Circulating BiomarkersUse of Nextgen to Study Circulating Biomarkers
Mostafa Ronaghi, Illumina Inc, speaking at Exosomes and Circulating Biomarkers Summit 2013.
Unravelling Pathogen Virulence Strategies through the Application of Transcriptomics and Real-time ImagingUnravelling Pathogen Virulence Strategies through the Application of Transcriptomics and Real-time Imaging
Professor Murray Grant, University of Exeter, speaking at AgriGenomics World Congress 2013.

Market Reports
DNA Sequencing
RI Technologies

This report presents an in depth analysis of DNA sequencing market. The study emphasizes on both, the methods and instruments/software of DNA sequencing in medical research, agriculture and animal husbandry and DNA testing and forensics application areas.
Big Data Market in India 2013
Netscribes

The report highlights the current as well as the future big data market scenario in India. The report furnishes explicit details on the crucial aspects of the overall market to provide a holistic view, with the aide of crisp and insightful representation of market data.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
DNA Sequencing 2013: Market Developments, Growth Areas and Opportunities
Biopharm Reports

DNA sequencing is important in all areas of biological research and increasing demand for this technique is driving advances in this field, in the form of next-generation techniques. Today, laboratories use a number of different sequencing methods, however common goals include increasing throughputs and speeds, while reducing costs.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
Benchtop Automation Trends 2013
HTStec

This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
Next-Gen Sequencing Trends 2012
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on next-generation sequencing (NGS) carried out in November 2012.
High Throughput qPCR Assay Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on high throughput real-time quantitative PCR (HT qPCR) assays carried out in January 2012.
The Future of Molecular Diagnostics: Innovative technologies driving market opportunities in personalized medicine
Business Insights

This report analyzes the current and potential world markets for molecular diagnostics, including advances in the field. This report generally forecasts future growth to 2015. The report breaks down market forecasts by type of test and by geography. It indicates which companies have key products in the market place. It also analyzes key drivers of this market.
Scientific News
Sequencing Efforts Miss DNA Crucial to Bacteria’s Disease Causing Power
New research suggests that current sequencing protocols overlook crucial bits of information: isolated pieces of DNA floating outside the bacterial chromosome.
Blueprint for the Affordable Genome
Stampede supercomputer powers innovations in DNA sequencing technologies.
Ubiquitous Protein Controls Copying of Resistant DNA
Researchers have demonstrated how the protein could put antibiotic-resistant bugs in handcuffs.
Common Bean Critically Important to the Global Food Supply
The bean genome sequence provides powerful tools to improve critical food crop.
Pocket Science: New Mobile Application Enables DNA Analysis On The Go
The application analyses ab1(1) DNA sequencing files, enabling science professionals and amateur enthusiasts to engage in scientific analysis from the convenience of their mobile phones.
Gene that Causes Obesity-Related Metabolic Syndrome Identified
Yale-led research has identified a genetic mutation responsible for the cluster of cardiovascular risk factors that comprise the obesity-related “metabolic syndrome.”
The 2014 Annual MO BIO Microbiome Awards, Offering More Than $10,000 of Prizes
The Microbiome Awards aim to provide young, extraordinary scientists with funding and recognition to carry out scientific work in the field of microbiome research.
Software Could Help Predict and Eliminate Allergens
It’s well known that allergies and asthma are on the rise around the globe, with food allergies in children alone rising 50% from 1997 to 2011.
Researchers Observe Overlap in Altered Genes Found in Schizophrenia, Autism and ID
Evidence supports theory that some cases of schizophrenia, autism and intellectual disability, malfunctions in some of the same genes are contributing to pathology.
Collaboration Unravels Novel Mechanism for Neurological Disorder
The novel gene (CLP1) associated with a neurological disorder affecting both the peripheral and central nervous systems.
Business News
NIH Funds $24M into Alzheimer’s Disease Genome Research
Scientists will analyze genome sequence data to identify gene risk, protective factors.
CSHL Receives $50M to Establish Simons Center for Quantitative Biology
Center will support research and education programs at one of the world’s leading independent biomedical research institutions.
BGI, University of Birmingham Create UK Environmental Omics Centre
The Centre will seek to protect environment, health and global biodiversity by analysing the toxicity of compounds more efficiently than has been achieved before.
Appistry Announces Pipeline Challenge Competition to Reward Creative Solutions for NGS Analysis
Winner will receive $70,000 worth of bioinformatics tools, software, hardware, and support to develop and build a production-grade pipeline.
Source BioScience Announces Appointment to the Board
Company announces that Pam Liversidge, OBE will join the Board as a Non-Executive Director with immediate effect.
BGI's NGS Products Receive CFDA Approval
These are the first next generation sequencing diagnostic products approved by CFDA.
Illumina Agreements to Expand Access for Non-Invasive Prenatal Testing Across Europe
Biomnis, Genoma, and the Center for Human Genetics and Laboratory Diagnostics Martinsried to use the HiSeq 2500 and consumables from Illumina to develop and perform non-invasive prenatal testing.
Roche Invests in Stratos Genomics
Investment by Roche will advance Stratos Genomics chemistry for nanopore sequencing.
TGAC at the Forefront of Next Generation Sequencing Capability
The Genome Analysis Centre adds two Illumina HiSeq 2500 machines to its platform suite.
TTP Labtech Revenues up 43%
The company has reported an impressive 43% increase in revenues and an associated increase in profits over 2013.

Events
NGS Workshop Sheffield 2014
16 Jul 2014
NGS Workshop Sheffield 2014 ...
More Information
5th EpiCongress Boston
22 Jul 2014
5th EpiCongress Boston ...
More Information
Enabling Genomic Technologies
11 Sep 2014
Enabling Genomic Technologies ...
More Information
Plant Genomics USA
11 Sep 2014
Plant Genomics USA ...
More Information

ePosters
Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
Institute of Progressive Medicine

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Agilent Technologies

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.
Improved Ligation Specificity with Chemically Modified Ligation Components
TriLink BioTechnologies, Inc.

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
Defining off-target cleavage in a pair of Zinc Finger Nucleases
University of Utah

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.
Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
BioTek Instruments

The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.
DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Epiontis GmbH and Genzyme Biosurgery

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.
EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
PentaBase

The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.
Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
Innovadyne Technologies, Inc

In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.
Application Notes
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Hamilton Robotics

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.
Molecular indexing for improved RNA-Seq analysis
Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.
An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.
Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Hamilton

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.
Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation
Bioo Scientific

The generation of high quality next generation sequencing data begins with libraries that have the desired insert size and proper adapter ligation.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.
Rapid and Reproducible DNA Isolation from 1 ml of Whole Blood with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

This application note shows the benefits of KingFisher Flex by using genomic DNA isolation from blood as an example.
Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software
Thermo Fisher Scientific

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.
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