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World of Technology & Science 2014...Recent Advancement in Mendelian Genomics...Next Generation Diagnostics...

Featured Products
Featured ProjectSentosa SX101
Sentosa SX101
Featured ProjectNEXTflex™ Rapid Directional qRNA-Seq™ Kit
NEXTflex™ Rapid Directional qRNA-Seq™ Kit

Webcasts
Characterizing the Early Drug Development Outsourcing Landscape Characterizing the Early Drug Development Outsourcing Landscape
Stella Stergiopoulos, Tufts Center for the Study of Drug Design, speaking at Drug Discovery and Development 2014
Recent Advancement in Mendelian Genomics and Data Management/Analysis Challenges at the Yale Center for Genome Analysis.Recent Advancement in Mendelian Genomics and Data Management/Analysis Challenges at the Yale Center for Genome Analysis.
Shrikant Mane, Yale Center for Genome Analysis, speaking at Advances in NGS & Big Data 2014

Market Reports
Gene Expression Analysis Market by Technology, Consumables, Services & Applications - Global Forecast to 2018
MarketsandMarkets

Over the years, the gene expression analysis market, comprising of instruments, consumables, and services, has witnessed various technological advancements. These advancements have led to a growth in the number of gene expression applications.
DNA Sequencing
RI Technologies

This report presents an in depth analysis of DNA sequencing market. The study emphasizes on both, the methods and instruments/software of DNA sequencing in medical research, agriculture and animal husbandry and DNA testing and forensics application areas.
Big Data Market in India 2013
Netscribes

The report highlights the current as well as the future big data market scenario in India. The report furnishes explicit details on the crucial aspects of the overall market to provide a holistic view, with the aide of crisp and insightful representation of market data.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
DNA Sequencing 2013: Market Developments, Growth Areas and Opportunities
Biopharm Reports

DNA sequencing is important in all areas of biological research and increasing demand for this technique is driving advances in this field, in the form of next-generation techniques. Today, laboratories use a number of different sequencing methods, however common goals include increasing throughputs and speeds, while reducing costs.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
Benchtop Automation Trends 2013
HTStec

This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
Next-Gen Sequencing Trends 2012
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on next-generation sequencing (NGS) carried out in November 2012.
High Throughput qPCR Assay Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on high throughput real-time quantitative PCR (HT qPCR) assays carried out in January 2012.
The Future of Molecular Diagnostics: Innovative technologies driving market opportunities in personalized medicine
Business Insights

This report analyzes the current and potential world markets for molecular diagnostics, including advances in the field. This report generally forecasts future growth to 2015. The report breaks down market forecasts by type of test and by geography. It indicates which companies have key products in the market place. It also analyzes key drivers of this market.
Scientific News
Brain-dwelling Worm in UK Man's Head Sequenced
Tapeworm removed from UK resident's brain reveals genetic secrets of an elusive Far East parasite.
Marching to Our Own Sequences
Study finds DNA replication timing varies among people.
New Test Developed at Dana-Farber/Brigham and Women's Cancer Center
Rapid Heme Panel - A high-tech genetic test that speeds treatment decisions for blood cancer patients.
Urgent Need for Fast Dx to Help Tackle Antibiotic Resistance
£10 million Longitude Prize for an accurate, rapid, cost-effective and easy-to-use point-of-need diagnostic test for bacterial infections.
Almac Announces Launch of CLIA Validated Next Generation Sequencing Assay
P53 considered important biomarker for cancer drug discovery.
Light Shed On Genetic Architecture Of Kidney Cancer
Research reveals link between renal cell carcinoma and exposure to aristolochic acid.
Large-scale Study Raises Hopes For Development Of E. coli Vaccine
Global comparison reveals surprisingly close relation between E. coli strains that cause traveller's diarrhoea.
DNA Sequencing Helps Identify Genetic Defects In Glaucoma
Scientists from the University of Liverpool have sequenced the mitochondrial genome in glaucoma patients to help further understanding into the genetic basis for the disease.
New Genetic Cause of Epilepsy Identified
San Diego girl improves after discovery of DNA mutation tied to potassium regulation.
Environmental Carcinogens Leave Distinctive Genetic Imprints in Tumors
Chemically induced tumors bear ‘smoking gun’ traces that sharply differentiate them from genetically engineered cancers.
Business News
Genotyping by Sequencing Training School
TGAC ran a four-day workshop aimed at introducing early stage scientists to genotyping by sequencing.
USAID, Broad Institute and Illumina Form a Public-Private Partnership
Partnership aims to combat the Ebola epidemic in West Africa.
QIAGEN Signs Ninth Master Collaboration Agreement for Companion Diagnostics
Master collaboration agreement is a framework for developing and commercializing QIAGEN companion diagnostics, paired with new or existing products.
Horizon Discovery Group plc Establishes Scientific Advisory Board for Horizon Diagnostics Business Unit
The Scientific Advisory Board will recommend improvements needed in laboratories adopting Companion Diagnostic assays.
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Pieces of the Puzzle - A Multi-method Approach to Tumour Profiling
OGT workshop provides insight on using complementary genomic technologies.
InVentis Technologies to Distribute TriLink Products in India
InVentis Technologies will market TriLink’s complete line of nucleic acid-based products.
Cartagenia Consortium Lungcadia Wins $1.76 M Grant
Cartagenia teams with BIOBASE and Hannover Medical School in Lungcadia project for cancer diagnostics and personalized therapy support.
Takara Bio Selects Genedata Software to Automate and Standardize NGS Data Analysis
Genedata Expressionist® for Genomic Profiling helps Takara Bio to manage, process, and analyze large NGS datasets.
Beckman, U of Birmingham Form Technology Collaboration
The partnership is aimed at advancing automation techniques for regulatory science research.

Events
2014 World Stem Cell Summit
03 Dec 2014
2014 World Stem Cell Summit ...
More Information
Prognostic, Predictive & Point
15 Jan 2015
Prognostic, Predictive & Point ...
More Information
RNA-Seq 2015
20 Jan 2015
RNA-Seq 2015 ...
More Information
Lab-on-a-Chip & Microfluidics
17 Mar 2015
Lab-on-a-Chip & Microfluidics ...
More Information

ePosters
Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks
Duke University

We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.

NEXTflex™ qRNA-Seq™ Molecular Indexing for ChIP-Seq and RNA-Seq
Bioo Scientific

Most Next Generation Sequencing (NGS) library prep methods introduce sequence bias with the use of enzyme processing and fragmentation steps can introduce errors in the form of incorrect sequence and misrepresented copy number. With molecular indexed libraries, each molecule is tagged with a molecular index randomly chosen from ~10,000 combinations so that any two identical molecules become distinguishable (with odds of 10,000/1), and can be independently evaluated in later data analysis.
A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Agilent Technologies

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
Institute of Progressive Medicine

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Agilent Technologies

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.
Improved Ligation Specificity with Chemically Modified Ligation Components
TriLink BioTechnologies, Inc.

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
Defining off-target cleavage in a pair of Zinc Finger Nucleases
University of Utah

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.
Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
BioTek Instruments

The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.
Application Notes
Automated Illumina TruSeq® Nano DNA Sample Preparation
Tecan Group Ltd.

The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. This low-input method generates excellent quality data from as little at 100 ng of DNA, allowing the study of samples with restricted DNA availability, for example from tumors, and helping to preserve precious sample material for use in future studies.
Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Hamilton Robotics

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.
Molecular indexing for improved RNA-Seq analysis
Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.
An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.
Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Hamilton

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.
Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation
Bioo Scientific

The generation of high quality next generation sequencing data begins with libraries that have the desired insert size and proper adapter ligation.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.
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