Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
Complete the form below to unlock access to ALL audio articles.
Abstract
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
The article is published online in the journal PLoS ONE and is free to access.