Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Life Technologies 400 base-pair Sequencing Kit for Ion PGM™ Sequencer

Published: Wednesday, December 19, 2012
Last Updated: Wednesday, December 19, 2012
Bookmark and Share
Kit delivers reads 60 percent longer than comparable high-throughput benchtop sequencers.

Ion 400 base-pair kit enables world-class bacterial assembly, HLA and 16S ribosomal gene sequencing, capping off another year of market-leading innovation.

Life Technologies Corporation announced a new 400 base-pair sequencing kit for the Ion PGM™ Sequencer. The kit produces reads 60 percent longer than comparable high-throughput benchtop sequencers, generating more complete bacterial de novo assemblies with longer contiguous sequences.

"Ion Torrent's long, accurate 400 base pair reads enable excellent bacterial de novo assembly," said Professor Dag Harmsen, Head of Research, Department of Periodontology, University of Muenster, who used the Ion PGM™ Sequencer to help crack the E. coli outbreak in Germany in 2011. "Paired-end sequencing is not required to get the best results."

The Ion PGM™ sequencing 400 kit is ideal for applications that require capturing all the genetic variation in a long exon on a single read. For instance, long reads are critical for researchers trying to interpret genetic data from human leukocyte antigen (HLA) genes or 16S ribosomal genes.

"Sequencing the V1-V2 region of the bacterial 16s rRNA gene would not be possible on the Ion PGM™ without the longer 400 base pair reads," said Dr. George Watts, Research Assistant Professor and Co-Director, Genomic Shared Service, University of Arizona Cancer Center. "Using 400 base pair reads allows us to increase the information density of the amplicons and better define the constituents of complex microbial populations."

Ion Torrent continues to see rapid increases in accuracy on its Ion PGM™ and Ion Proton™ Sequencers as a result of consistent improvements in its algorithms. The new Torrent Suite v3.4 incorporates algorithmic improvements in both raw accuracy and consensus variant calling. These improvements enable both the Ion PGM™ and Ion Proton™ semiconductor sequencing platforms to routinely achieve greater than 99.996 percent consensus accuracy.

Life Technologies is also introducing two additional products, capping a year of extraordinary innovation that made the Ion PGM™ Sequencer the fastest-selling sequencer in the world:
•    The Ion Total RNA-Seq Kit for AB Library Builder™, which is designed to make cDNA library preparation for the semiconductor sequencing simple, fast and flexible. The Ion Total RNA-Seq Library Kit for AB Library Builder™ System enables the construction of cDNA libraries from both whole transcriptome and purified small RNA (miRNA) populations, allowing researchers to analyze all types of RNA molecules.  
•    The Thermo Scientific® MuSeek™ Library Preparation Kit, provides a fast and simple transposon-based method for preparing high-quality genomic DNA libraries for the Ion PGM™ Sequencer. The kit utilizes MuA transposase enzyme for fragmentation and simultaneous tagging of the target DNA, eliminating the need for separate shearing and adaptor ligation steps. Life Technologies is excited to offer this new kit, which enables library construction in as little as 90 minutes, resulting in significant savings in time and cost.

The Ion Torrent products discussed in this press release are for Research Use Only, not for use in diagnostic procedures.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Life Technologies' Ion AmpliSeq™ Exome Program Adds 12 New Members
With more than 30 members the program is the largest network of next generation-based exome sequencing providers in the world.
Friday, December 20, 2013
KACST Partners with Life Tech in The Saudi Human Genome Program
Program aims to analyze 100,000 human genomes to identify the causes of disease in the population of the Kingdom of Saudi Arabia and the Arab world.
Monday, December 09, 2013
BGI Partners with Life Technologies
World's largest genome center to purchase 50 Ion Proton™ Sequencers from Life Technologies.
Tuesday, October 22, 2013
Life Technologies Expand Ion AmpliSeq™ Exome Certified Service Provider Program
Due to increased demand the program is expanding to include eleven new members.
Wednesday, October 09, 2013
TriCore Reference Laboratories Named First NGS Center of Excellence by Life Technologies
Collaborative partnership designed to accelerate clinical research in the Southwest using advanced NGS oncology panels.
Thursday, September 19, 2013
Life Technologies Signs Five-Year Agreement with the FDA
The agreement aims to accelerate and advance food safety testing of E. coli and Salmonella, two foodborne contaminants commonly associated with outbreaks and/or recalls.
Tuesday, July 30, 2013
Life Technologies to Collaborate with Merck Serono
Long-term collaboration is aimed at developing, obtaining regulatory approval and commercializing companion diagnostics for selected Merck Serono drug development programs.
Wednesday, July 17, 2013
Life Technologies Acquires Life Science Korea
The acquisition strengthens Life Tech's go-direct strategy in South Korea.
Friday, June 14, 2013
Roswell Park Cancer Institute Purchases Six Ion Torrent Sequencers
Life Technologies Corporation announced that the new Center for Personalized Medicine at Roswell Park Cancer Institute has purchased four Ion Proton™ sequencers and two Ion Personal Genome Machine™ sequencers.
Thursday, February 21, 2013
Life Technologies Bolsters its 60 Percent Market Share Lead in Benchtop Sequencers
More than 90 peer-reviewed papers and the world's largest online sequencing community reflects broad adoption of Ion Torrent™ technology.
Wednesday, February 20, 2013
Life Technologies Receives FDA 510(k) Clearance for Diagnostic Use of Products
Life's Applied Biosystems Dx Genetic Analyzer and SeCore® kits constitute first sequence-based system cleared for HLA typing.
Tuesday, February 12, 2013
Boston Children's Hospital and Life Technologies Launch Claritas Genomics, a Clinical Genomics Company
Spinoff venture will develop genomic sequencing solutions utilizing Ion Torrent technology for clinical applications.
Thursday, January 10, 2013
Life Technologies Announces Third Quarter 2012 Results
Revenue for the third quarter was $911 million, a decrease of 1.9 percent over the $929 million reported for the third quarter of 2011. Excluding the impact of currency, revenue growth for the quarter was 1.4 percent compared to the same period of the prior year.
Monday, November 05, 2012
VelaDx Signs Agreement with Life Technologies to Develop IVD Tests on Ion PGM Sequencer
Semiconductor sequencing brings unprecedented power to diagnostic tests to transform personalized medicine.
Wednesday, October 10, 2012
Life Technologies Acquires Compendia Bioscience
Bioinformatics expertise will enable deeper engagement with established customer base of leading pharmaceutical companies.
Tuesday, October 09, 2012
Scientific News
Decoding the Genome of the Olive Tree
A team of scientists from three Spanish centers has sequenced, for the first time ever, the complete genome of the olive tree. This work will facilitate genetic improvement for production of olives and olive oil, two key products in the Spanish economy and diet.
Four Newly-Identified Genes Could Improve Rice
A Japanese research team have applied a method used in human genetic analysis to rice and rapidly discovered four new genes that are potentially significant for agriculture. These findings could influence crop breeding and help combat food shortages caused by a growing population.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Biomarkers That Could Help Give Cancer Patients Better Survival Estimates Discovered
UCLA research may also help scientists suppress dangerous genetic sequences.
Mobile Laboratories Help Track Zika Spread Across Brazil
Researchers from the University of Birmingham are working with health partners in Brazil to combat the spread of Zika virus by deploying a pair of mobile DNA sequencing laboratories on a medical ‘road trip’ through the worst-hit areas of the country.
How “Silent” Genetic Changes Drive Cancer
The researchers found that EXOSC2 expression is enhanced in metastatic tumors because their cells have increased levels of a tRNA called GluUUC.
‘Jumping Gene’ Took Peppered Moths To The Dark Side
Researchers from the University of Liverpool have identified and dated the genetic mutation that gave rise to the black form of the peppered moth, which spread rapidly during Britain’s Industrial Revolution.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
How Did The Giraffe Get Its Long Neck?
Clues about the evolution of the giraffe’s long neck have now been revealed by new genome sequencing.
Big Data Can Save Lives
The sharing of genetic information from millions of cancer patients around the world could be key to revolutionising cancer prevention and care, according to a leading cancer expert from Queen's University Belfast.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!