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New NEXTflex Rapid DNA-Seq Kit Offers Two-Hour NGS Library Construction from 1 ng of DNA

Published: Monday, June 24, 2013
Last Updated: Monday, June 24, 2013
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Bioo Scientific’s NEXTflex™ Rapid DNA-Seq Kit allows researchers to build sequencing -ready libraries for sequencing on Illumina’s sequencing platforms.

This kit is ideal for use with genomic DNA, as well as FFPE samples, ChIP DNA and clinical samples.

The NEXTflex Rapid DNA-Seq Kit offers the flexibility to use between 1 ng - 1 µg of input DNA. A bead-based, gel-free size selection protocol eliminates the need for agarose gels in size selection, and the availability of up to 96 unique adapter barcodes facilitates high-throughput applications. The NEXTflex Rapid DNA-Seq Kit incorporates “Enhanced Adapter Ligation Technology”, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientific’s NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance.

According to Dr. Masoud Toloue, Director of Genomic Research at Bioo Scientific, “The NEXTflex Rapid DNA-Seq Kit offers the perfect balance of simplicity and speed without sacrificing library fidelity. This is achieved by optimizing the enzymatic reactions to allow multiple steps to be run simultaneously while avoiding bias that is inherent with enzymatic fragmentation and transposases.”

Bioo Scientific is an industry leader in the design and development of technology that minimizes bias and allows high levels of sample multiplexing to increase the efficiency and reduce the costs of NGS. The NEXTflex Rapid DNA-Seq Kit is the latest addition to Bioo Scientific’s line of innovative kits for next generation sequencing library preparation.

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