Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Lucigen, Thermo Fisher to Release New Mate Pair Library Technology

Published: Wednesday, August 27, 2014
Last Updated: Wednesday, August 27, 2014
Bookmark and Share
The Ion PGM is a fast and cost-effective platform for next generation sequencing applications.

The release of the Ion TrueMate™ Library Kit will expand the platform’s capabilities by allowing long mate pair libraries up to 8 kb to be run on the PGM. Mate pair libraries are critical for de novo genome assembly, chromosomal rearrangement studies, and finishing genomes. Traditional fragment libraries produce large quantities of data but result in sequence gaps, misassembled contigs, and collapsed repeats due to short read lengths.  Combining mate pair libraries with fragment libraries has been proven to achieve more accurate genome assemblies.

Developed at Lucigen using their NxSeq® mate pair technology, the Ion TrueMate Library Kit includes multiple innovations that enable high fidelity mate pair libraries without paired end sequencing on the PGM platform.  The protocol and reagents have been optimized to eliminate chimera formation, with the inclusion of patent-pending ChimeraCode™ sequences allowing confirmation of actual mate pairs via bioinformatics.  The TrueMate libraries demonstrate >90% efficiency of real mate pairs, compared to 30% or less with other platforms.  Lucigen’s new Accura™ high fidelity polymerase is also incorporated into the new product.

“Existing mate pair libraries contain a high percentage of chimeras that result in misassembled genomes. The mate pairs that are obtained from the TrueMate libraries achieve >90% efficiency, and when combined with Ion Fragment libraries, have shown tremendous promise for closing genomes, finishing BAC sequences, and obtaining accurate scaffold assemblies,” states Lucigen CEO and Founder Dr. David Mead.

The TrueMate Library Kit will be sold for the PGM platform exclusively through Thermo Fisher, while Lucigen will perform library creation and sequencing services for customers globally.

The release of the Ion TrueMate library kit is the first of multiple mate pair kits in development by Lucigen.  Future products will include the ability to create longer mate pairs of 20 kb or higher and the application of whole transcriptome sequencing via Full Length RNASeq protocols.

For research use only.  Not for use in diagnostic procedures.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
New Neurodevelopmental Syndrome Identified
Study pinpoints underlying genetic mutations, raising hopes for targeted therapies.
Uncovering Hidden Genomic Alterations that Drive Cancers
Tested on large tumor genomics database, REVEALER method allows researchers to connect genomics to cell function.
Gene Behind Rare Childhood Syndrome Identified
Online activism by one patient’s mother spurred research collaboration which led to the identification of a new genetic syndrome.
Resilience Project Identifies Rare Unaffected Individuals
Researchers from Mount Sinai and Sage Bionetworks report analysis of nearly 600,000 genomes for resilience project.
Rare DNA Will Have Nowhere To Hide
Two National Institutes of Health grants back Rice University effort to develop new diagnostics.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Children With Cancer To Get New Gene Test
Pilot study will sequence 81 cancer genes in children’s tumours to help personalise cancer treatment.
How The Bat Got Its Wings
Finding may provide clues to human limb development and malformations.
Lupus Study Shows Precision Medicine’s Potential to Define the Genetics of Autoimmune Disease
Researchers at UT Southwestern have used next-generation DNA sequencing technology to identify more than 1,000 gene variants that affect susceptibility to SLE.
Illuminating the Broad Spectrum of Disease
PRISM efficiently tests drug compounds in multiple cell lines simultaneously, accelerating discovery of targeted therapies in the service of precision medicine.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!