|A multiplexed amplicon sequencing technology for FFPE and circulating, cell-free DNA|
Laurie Kurihara, Catherine Couture, Julie Laliberte, Sukhinder Sandhu, Jonathan Irish, Tim Harkins and Vladimir Makarov
A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.
|Reducing Biases in Small RNA Sequencing|
Adam Morris Ph.D., Dawn Obermoeller, Masoud Toloue Ph.D.
Small RNA-Seq libraries utilizing randomized adapters demonstrated significantly less bias and more even coverage due to reductions in ligase bias.
|Amplicon Based 16S Ribosomal RNA Sequencing and Genus Identification|
*Jan Risinger, *Lindsey Renken, +Josh Hill, +Noushin Ghaffari, PhD, +Richard P. Metz, PhD, +Charles D. Johnson, PhD, *Masoud M. Toloue, Ph
Rapid determination of highly complex bacterial populations through targeted amplification of 16S rRNA V1 - V3 hypervariable regions can provide an accurate gauge of diversity at taxonomic hierarchies as low as the genus level.
|Stable Chloroplast: Myth or Reality?|
Shailesh Joshi and Dyfed Evans
Chloroplasts principally encode the photosynthetic machinery in Viridiplantae. It has long been accepted that in photosynthetic plants chloroplast genomic structure is uniquely stable as it is maternally and clonally inherited. The first chloroplast genomes sequenced supported this view. The current study was undertaken to address the potential issue of global chloroplast(in)stability.
|Geneious R8: A Powerful and Comprehensive Suite of Molecular Biology Tools
Christian Olsen, Kashef Qaadri, Richard Moir, Matt Kearse, Simon Buxton, Matthew Cheung, Hengjie Wang, Jonas Kuhn, Steven Stones-Havas, Chris Duran
Geneious R8: A powerful and comprehensive suite of molecular biology tools.
|Putative Genes Identified on Two Growth Conditions of G. boninense|
Jayanthi N, Abrizah O, Low ETL, O-Abdullah M, Hogan M, Cuomo CA, Desjardins C, Abdul Manaf MA, Rajinder S, Birren B and Ravigadevi S
Putative genes identified on two growth conditions of Ganoderma boninense.
|Contrasting Patterns of Neutral and Adaptive Genetic Variation of Chilean Blue Mussel (Mytilus chilensis) Due to Local Adaptation and Aquaculture|
Cristian Araneda1 , M. Angelica Larrain2, Benjamin Hecht3, Shawn Narum4
This study was designed to investigate patterns of neutral and adaptive genetic variation within Chilean blue mussel populations in order to identify a subset of putatively adaptive genetic markers to investigate the population structure and to improve the ability to trace individuals to their geographical origin, especially in the area with strong aquaculture activities
|Transcription Factors to Classify Tumor Types and Subtypes|
Benjamin Otto 1,2, Kristin Klätschke 2, Thomas Streichert 3, Christoph Wagener 2, Genrich Tolstonog 4
Here we introduce the use of the unsupervised approach to identify transcription factors (TFs) that are specific for different tumor types.
|Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks|
Vishwa Nellore, Chris Dwyer
We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.
|NEXTflex™ qRNA-Seq™ Molecular Indexing for ChIP-Seq and RNA-Seq|
Jan Risinger, Masoud Toloue Ph.D.
Most Next Generation Sequencing (NGS) library prep methods introduce sequence bias with the use of enzyme processing and fragmentation steps can introduce errors in the form of incorrect sequence and misrepresented copy number. With molecular indexed libraries, each molecule is tagged with a molecular index randomly chosen from ~10,000 combinations so that any two identical molecules become distinguishable (with odds of 10,000/1), and can be independently evaluated in later data analysis.
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