High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam IncheRecent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay. |  | |
Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard HogrefeLigases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation. | | |
Defining off-target cleavage in a pair of Zinc Finger Nucleases
K. Mukherjee, D. CarrollThis study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs. | | |
Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
Wendy Goodrich, Jason Greene, Mary Louise ShaneThe ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities. | | |
DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven OlekWe demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative. | | |
EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
K. Skadhauge, C. Nielsen & U.B. ChristensenThe EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts. | | |
Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
Mary Cornett, Mitch Gordon and Anca RotheIn this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation. | | |
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A Cautionary Tale on Genome-Sequencing Diagnostics for Rare DiseasesStudies in several children have raising new questions about inheritance, genomic sequencing, and diagnostic.
Gene Identified Responsible for Disorders of Bones and Connective TissueResearchers have identified a gene that when mutated is responsible for a spectrum of disorder.
Genetic Study Shows Europeans are One Big FamilyEuropeans are basically one big family, closely related to one another for the past thousand years, according to a new study of the DNA of people from across the continent.
Genome Sequencing Provides Unprecedented Insight into Causes of Pneumococcal DiseaseTechnology will allow better surveillance of bacterial populations, understanding of vaccine effectiveness.
Tick-Borne Lone Star Virus Identified through New Super-Fast Gene SequencingThe tick-borne Lone Star virus has been conclusively identified as part of a family of other tick-borne viruses called bunyaviruses, which often cause fever, respiratory problems and bleeding.
Launch of £90m Initiative in Big Data and Drug Discovery at Oxford University'Big data' to revolutionise healthcare.
Why Does Smallpox Vaccine Shield Some, Not Others? It's in the GenesHow well people are protected by the smallpox vaccine depends on more than the quality of the vaccination: individual genes can alter their response, Mayo Clinic research shows.
Gifts to Boost University of Chicago as Hub for Biomedical 'Big Data'Two major gifts will build momentum behind the University of Chicago’s leadership in biomedical computation.
AD GWAS in African Americans Confirms, Reshuffles AlzGene ListAfrican Americans carrying a variant in a cholesterol-processing gene have double the risk for Alzheimer’s than non-carriers.
New System to Improve DNA SequencingA sensing system developed at Cambridge is being commercialised in the UK for use in rapid, low-cost DNA sequencing.
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