The Pippin Prep is a preparative electrophoresis platform that separates and extracts DNA fragments. Using pre-cast and disposable gel cassettes, DNA is automatically collected in buffer according to software-input size ranges. DNA fractions (typically adapter-ligate NGS libraries) are removed using standard pipettes. Workflow requires about 1-2 minutes of hands on time per sample, and cassettes are available for extractions between 50 bp and 8kb. Typical run times (four samples per run) are between 50 and 100 minutes.
Benefits of the Pippin Prep™ DNA size selection system
• Minutes of labor saves time compared to manual gel purification • Reproducible extractions provide more consistent results • Provides narrow fragment size distributions for paired-end sequencing • Minimal low molecular weight contamination reduce wasted reads and ambiguous indel calling • Individual sample channels eliminate sample cross-contamination