Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>Resources>White Papers>This White Paper
  White Papers
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Accelerating the Detection of Foodborne Bacterial Outbreaks
The speed of diagnosis of foodborne bacterial outbreaks could be improved by a new technique developed by researchers at the Georgia Institute of Technology.
Antibiotics Discovered While Sifting Through Human Microbiome
Researchers identify genes in a microbe’s genome that produce antibiotic compounds, then synthesize them without the need for bacterial culture.
A Genome-wide View of Human DNA Viruses
In this study, Duplex sequencing was used to accurately analyse the genome-wide rate of spontaneous mutation of human adenovirus C5 (HAdv5).
Peer Review is in Crisis, But Should be Fixed, Not Abolished
After the time to get the science done, peer review has become the slowest step in the process of sharing studies, and some scientists have had enough.
Rare Immunodeficiency Yields Unexpected Insights
Scientists uncover previously unknown gene mutation revealing the role of a key molecule involved in immune cell development.
Illumina Contributes to ClinVar Database
The contribution includes variants of all classifications, from pathogenic to benign, identified during interpretation of whole genome sequences generated in the CLIA-certified, CAP-accredited Illumina Clinical Services Laboratory.
Agilent Presents Early Career Professor Award to Dr. Roeland Verhaak
JAX professor recognized for the development and implementation of workflows for the analysis of big-data from transcriptomics to next generation sequencing approaches.
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Scroll Up
Scroll Down

Next-generation sequencing of highly multiplexed samples: the NuGEN Encore™ 384 Multiplex System
Bookmark and Share

NuGEN Technologies, Inc.

Advances in DNA sequencing throughput afforded by highly parallel, reversible-terminator sequencing platforms have completely redefined our view of biological inquiry. Crisp, high-resolution, digital views of whole-genome biology provide both profound clarity and significance. As with any disruptive technology, implementation creates turbulence. In the case of NGS, accelerated sequencing throughput has created challenges in upstream sample preparation and downstream data analysis. Initially, sample preparation was particularly problematic because it required individuals with highly specialized molecular biology skills for the hands-on execution of complex protocols. Fortunately, automated solutions for NGS library preparation have arrived, and it is reasonable to expect broad dissemination of turnkey solutions for routine library generation in the very near future. What remains problematic is that some sequencing projects involve large numbers of samples that require relatively shallow sequencing coverage. The sequencing costs of such projects are often prohibitive, calling for methods to multiplex high sample numbers in the same sequencing run. The obvious solution is to combine many samples into a single sequencing lane, and there are now many reports in the literature describing ad hoc methods and strategies for multiplexing samples prior to sequencing. Here we introduce a commercial solution for deep multiplexing of NGS samples that builds on our Encore™ NGS Multiplex System. The Encore 384 Multiplex System consists of a refined set of 384 molecularly “bar-coded” library adaptors that enable deep multiplexing of sequencing samples within a library preparation system that is compatible with high-throughput automation. The version of the product discussed in this White Paper contains adaptor sequences compatible with Illumina sequencing platforms.

Further Information


Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!