Corporate Banner
Satellite Banner
Technology Networks Header
Friday, August 22, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Using Next-Gen Sequencing to Uncover Patterns of Allele-Specific Expression
  Videos

Return

Using Next-Gen Sequencing to Uncover Patterns of Allele-Specific Expression
University of California

Allelic imbalance refers to the unequal expression from maternal and paternal chromosomes of non-imprinted genes and has been shown to be a genome-wide phenomenon. Allelic imbalance can now be accurately measured by analyzing abundance of coding heterozygous SNPs from RNA sequencing and can range from 1:2 to more than 1:64; yet the mechanisms involved are not completely understood. In my presentation I will discuss data from next generation sequencing of CD4+ T cells from a pair of monozygotic twins discordant for the autoimmune disorder multiple sclerosis showing differential allelic expression in several genes. In many cases, the index variants are close to transcription factor binding sites in heavily methylated areas of the genome. However, the correlation with differential methylation patterns is not straightforward, thus suggesting a more complex regulatory mechanism is involved in allelic expression.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Sequencing Identifies Gene Variant Responsible for Lupus
Research demonstrates it is feasible to identify the individual causes of lupus in patients by using DNA sequencing, allowing doctors to target specific treatments to individual patients.
New Material Could Enhance Fast and Accurate DNA Sequencing
Nanopores in the material MoS2 sequence DNA more accurately, quickly and inexpensively.
ChIP Sequencing Kit for Next Generation Sequencing
The assay kit from Porvair Sciences extends the benefits of their proprietary Chromatrap® solid state ChIP technology.
Illumina's HiSeq X Ten Sequencing Technology for Population Studies
Next-generation sequencing technologies to be implemented at new High-Throughput Genomics Center for the MENA region.
Foodborne Pathogen Detection Speeds Up Dramatically
Next-generation sequencing techniques allow rapidly identification of strains of salmonella, quickening responses to potential outbreaks.
Sequencing Efforts Miss DNA Crucial to Bacteria’s Disease Causing Power
New research suggests that current sequencing protocols overlook crucial bits of information: isolated pieces of DNA floating outside the bacterial chromosome.
Blueprint for the Affordable Genome
Stampede supercomputer powers innovations in DNA sequencing technologies.
Ubiquitous Protein Controls Copying of Resistant DNA
Researchers have demonstrated how the protein could put antibiotic-resistant bugs in handcuffs.
Common Bean Critically Important to the Global Food Supply
The bean genome sequence provides powerful tools to improve critical food crop.
Pocket Science: New Mobile Application Enables DNA Analysis On The Go
The application analyses ab1(1) DNA sequencing files, enabling science professionals and amateur enthusiasts to engage in scientific analysis from the convenience of their mobile phones.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv