|Acoustophoretic microfluidic device for high throughput DNA sequencing|
V.V Unnikuttan1, H.N Unni 1
Multiphysics modelling for acoustic standing wave technology combined with micro-technology which can be used for manipulation and concentration on typical Lab-on-Chip devices for DNA sequencing.
|Development of a Paper-Based Fluidic Device for Phosphorus Detection|
Patricia K. Rusch and Kyle A. Cissell
This poster displays the successful beginning stages of an innovative way to detect phosphorus in standing bodies of water by minimizing the quantity of chemicals used, reducing the cost of analysis instrumentation, allowing the potential for real-time monitoring, and producing consistent and reliable results.
|Assessment of the efficiency of encapsulation of a fluorescent drug using Nanoparticle Tracking Analysis|
Patrick Hole, Pierre Peotta, Roberto Santoliquido, Bob Carr
This poster outlines an example where the methodology of Nanoparticle Tracking Analysis (NTA) is used to characterise nanoparticles for drug delivery purposes.
|Single Cell Whole Genome Amplification|
The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses the a technology for amplification of genomic DNA form a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase
|The Prestwick Chemical Library (R), A Valuable Tool for Screening|
Jean-Marie Contreras1, Christophe Morice1, Jean-Marc Simon1, Bruno Didier2, Marie-Louise Jung1 and Thierry Langer3
The Prestwick Chemical Library® (PCL) is Prestwick’s flagship product dedicated to screening. It is a collection of 1280 molecules comprising 100% approved drugs (FDA, EMEA and other agencies) selected for their high chemical and pharmacological diversity. The PCL was designed to reduce the risk of "low quality" hits and therefore the cost of the initial screening, and appears to be an efficient smart library for hit discovery. The PCL comes with an extended fully-annotated database.
|System-Specific Periodicity in qPCR Data and its Impact on Quantitation|
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3
In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.
|Bovine RNA-seq data analysis of liver and pituitary gland|
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.
Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.
|A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction|
Juan Triviño Paredes1, Atilla Biçer1, Arnauld Besson2, Edurne Gallastegui1, Josep Maria Estanyol3, Maria Jesus Pujol1 and Oriol Bachs1
A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction
|Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult|
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1
We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.
|Study Of The Active Bacterial Community In Two Membrane Bioreactors|
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).
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