|Busted! First Dection of Steroid Hormones in Pacific Walrus Bones|
Charapata P, Horstmann L, and Misarti N
The Pacific walrus (Odobenus rosmarus divergens) is a candidate species for the Endangered Species List because Arctic sea ice has declined significantly in the past decades. This project focuses on validating a method for extracting steroid hormones from archaeological, historical, and modern bone creating a baseline of hormone levels during different climate regimes. The results validate our method of hormone extraction as all steroid hormones were extracted from bones of all time frames.
|Ichthyophonus vs. Proteins: Should we be eating these fish?|
The project, Ichthyophonus vs. Proteins, analyzes the effect of Ichthyophonus on the protein content of Halibut from Homer. This experiment found that Ichthyophonus does not have a significant effect on the protein content of the tested Halibut. The findings confirm the belief that Ichthyophonus is not harmful to humans and the Halibut are still safe to eat.
|Uncertainty estimation of an analytical method for analysis of fumonisin B1 in four types of rice intended for consumption human|
Petrarca, M.H.; Rossi, E.A.; Sylos, C.M.
The expanded measurement uncertainty of a sample preparation method based on QuEChERS procedure for the determination of fumonisin B1 in four types of rice, using HPLC-FLD after precolumn derivatisation with ortho-phthaldialdehyde, was estimated from in-house validation data.
|TFAP2B is Associated with WNT/ß-catenin Pathway in Luminal Breast Cancer|
Benjamin Otto1,2, Kristin Klätschke2, Thomas Streichert3, Christoph Wagener2, Genrich Tolstonog4
Here we aimed to investigate which molecular mechanisms are associated with TFAP2B expression in luminal breast cancer.
|Transcription Factors to Classify Tumor Types and Subtypes|
Benjamin Otto 1,2, Kristin Klätschke 2, Thomas Streichert 3, Christoph Wagener 2, Genrich Tolstonog 4
Here we introduce the use of the unsupervised approach to identify transcription factors (TFs) that are specific for different tumor types.
|Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry|
SC Cribbes, S Brightwell, K Kaji
Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
|Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method|
Sarah Kessel, Eric Sincoff, Olivier Dery
This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.
|Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.|
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer
We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells
|Forced Degradation Study of Expired and Marketed Tablets of Amlodipine by RP-HPLC|
Manisha Puranik*, Monilisha Varma
The objective of the current study was to develop simple, accurate, sensitive and stability indicating RP-HPLC method for the determination of per cent drug remained of Amlodipine besylate in the presence of its degradation products in bulk drug, expired tablets and tablets whose expiry date has not been exceeded.
|LC-MS/MS METHOD FOR THE QUANTIFICATION OF PITOFENONE IN HUMAN PLASMA|
B.M. Gurupadayya, S. Sharfuddin and R.S. Chandan
We developed LCMS/MS bioanalytical method for quantitative estimation of Pitofenone HCl in plasma is simple, sensitive, accurate, precise, and selective. Percentage recovery shows that the method is free from interference of matrix. The analytical method presented here has proved to be useful for investigation of the characteristics of Pitofenone in human plasma in pharmacokinetic and pharmacogenetic studies.
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