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Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
Stefanie Buesch1 , John Langer2 , Sabine Schaepermeier1, Lubna Hussain2, Jeffrey Bergeron3, Volker Vogel1, Jenny Schroeder1

This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay.

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Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System
Cecile Villemant1 , Sabine Schäpermeier2 , Stefanie Büsch2 , John Langer3 , Theresa D’Souza3 , Lubna Hussain3 , Grant Cameron1 , Volker Vogel2 , Jenny Schroeder2

This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures.

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Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System
John Langer1 , Jenny Schroeder2 , Lubna Hussain1 , Claudia Schwartz2 and Theresa D’Souza1

The RAFT™ 3D cell culture system provides a valuable tool to investigate different cell types singularly or in co-cultures in an in-vivo like collagen based microenvironment.

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Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in Clonetics™ HUVEC using the Lonza 4D-Nucleofector™ System
Srinivasan Kokatam1 , Kanchan Tiwari1 , Jenny Schroeder2 , Andrea Toell2 , Lubna Hussain3, Preeti Kapoor1

In the current study we have used siRNA targeting VEGFR2 as an example to study knockdown of VEGFR2 and subsequent inhibition of tube formation by HUVECs on Growth Factor Reduced Matrigel™ in a 96-well plate format. The same strategy can be used for screening and validating siRNA based inhibitors of the angiogenic process in vitro and thus could be of utility in anti-cancer screening strategies.

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Pharmacological Responses in Cultured Human iPSC-Derived Cortical Neurons Using Multi-Electrode Array
Aoi Odawara (1,2), Hiroki Katoh (1), Naoki Matsuda (1), Karolina Szczesna (3), Yichen Shi (3), Ryan Arant (4), Hideyasu Jiko (4), Ikuro Suzuki (1)

The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs).

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Clinical Applications of “Liquid biopsy” in the Colorectal Cancer Treatmen
Koichi Suzuki, Yuji Takayama, Kosuke Ichida, Taro Fukui, Nao Kakizawa, Fumiaki Watanabe, Fumi Hasegawa, Rina Kikugawa, Shingo Tsujinaka, Yasuyuki Miyakura, Toshiki Rikiyama

Liquid biopsy provides a circulating biomarker not only for treatment response but decision making of a sequential strategy.

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Targeting Acute Pancreatitis by Small Molecule Inhibitors of Cyclophilin D
M. Awais, E. Shore, R. Gibson, N. Kershaw, D. Latawiec, S. Pandalaneni, M.A. Javed, L. Wen, D.N. Criddle, N. Berry, L-Y. Lian, P. O’Neill, R. Sutton

Cyclophilin D (CypD) promotes opening of the mitochondrial permeability transition pore, a major contributor to acute pancreatitis. We are developing small molecule inhibitors of CypD as a possible treatment for AP and other conditions where the MPTP plays a role.

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Instrumental Investigations: A Laboratory Manual of Forensic Analytical Chemistry
Robert Q. Thompson

A laboratory manual for undergraduate analytical and forensic chemistry courses

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The Pathogen Box: A Catalyst for Neglected Disease Drug Discovery
Benoît Laleu*, Thomas Spangenberg, Wesley Van Voorhis, Angelique Doy, Dylan Pillai, Andreas Verras, Joie Garfunkle, Jeremy Burrows, Timothy Wells, Paul Willis

Modelled after the Malaria Box, the Pathogen Box contains ~400 diverse drug-like molecules active against neglected diseases of interest such as Chagas disease, cryptosporidiosis, hookworm, sleeping sickness, leishmaniasis, lymphatic filariasis, malaria, onchocerciasis, schistosomiasis, trichuriasis, tuberculosis.

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Rapid electrochemical impedance spectroscopy for protein detection in Lab-on-a-Chip devices
T. Pardy(a); N. Sleptsuk(a); M. Min(a); J. Ojarand(a); T. Lakspere(a,b); K. Palm(b)

We present results in rapid solution impedance spectroscopy to detect protein interactions (antibody-antigen) in human serum. Two different serum-antibody mixtures are measured in cuvettes, interfaced with screen-printed electrodes to determine whether the experimental setup can detect differences in composition.

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Scientific News
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Genetic Markers Influence Addiction
Differences in vulnerability to cocaine addiction and relapse linked to both inherited traits and epigenetics, U-M researchers find.
Lab-on-a-Chip for Detecting Glucose
By integrating microfluidic chips with fiber optic biosensors, researchers in China are creating ultrasensitive lab-on-a-chip devices to detect glucose levels.
A lncRNA Regulates Repair of DNA Breaks in Breast Cancer Cells
Findings give "new insight" into biology of tough-to-treat breast cancer.
COPD Linked to Increased Bacterial Invasion
Persistent inflammation in COPD may result from a defect in the immune system that allows airway bacteria to invade deeper into the lung.
Detection of HPV in First-Void Urine
Similar sensitivity of HPV test on first void urine sample compared to cervical smear.
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