|The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry|
Xiaoqin Cheng, Kristina Larson, Letitia Kwok, David Mierzejewski, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney, David Kitchen, Amanda Haas, Melissa Kelley, Anja van Brabant Smith
Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.
|Development and Application of Quantitative Immunoassays for Major Milk Allergens Bos d 5 (β-lactoglobulin) and Bos d 11 (β-casein)|
1Ross A. R. Yarham, 1Anna Kuklinska-Pijanka MSc, 1David Gillick, 1Elizabeth Young, 2Karine Adel Patient PhD, 2Hervé Bernard PhD, 1Martin D. Chapman PhD, 1James P. Hindley PhD
In this study, we sought to develop accurate, sensitive and reliable assays that would enable quantification of multiple milk allergens.
|Blood Transcriptomic Diagnosis of Active TB with Five Genes|
JK Roe1*, N Thomas1, E Gil1, K Best1, E Tsaliki1, S Morris-Jones2, S Stafford1, N Simpson1, KD Witt3, B Chain1, R Miller4, A Martineau3, M Noursadeghi1
This study aimed to identify the minimal set of genes that can accurately diagnose active TB, both pulmonary and extrapulmonary, and evaluate their specificity.
|A Novel Benchtop Time-of-Flight GC-MS System for High Performance Analysis of Human Urine|
David E. Alonso, Joseph E. Binkley, Lorne M. Fell
A workflow was developed and implemented for the effective comparison of urine. It involved the preparation of stable compounds through derivatization, data acquisition using a high performance benchtop TOFMS, comprehensive data processing (NonTarget Deconvolution™), and quick retrospective analysis of rich datasets using Target Analyte Finding.
|Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA|
Hidevaldo B. Machado, John A. Schiel, Maren Mayer-Gross, Eldon T. Chou, Melissa L. Kelley, Anja van Brabant Smith. Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Precise genome engineering with CRISPR-Cas9 and single-stranded DNA oligo or double-stranded DNA plasmid donors via homology-directed repair (HDR).
|Magnetically Manipulated Sample Handling System on Digital Microfluidic Platforms|
Debiprasad Chakrabarty1, 2, Susovan Dutta2, Niladri Chakraborty2, Ranjan Ganguly2
Herein we present a proof of concept demonstration of magnetic manipulation of an immiscible, microliter-scale ferrofluid droplet over a thin aqueous film on a solid substrate, using an array of square electromagnets.
|Comparison of Key Signaling Pathways in Lung Cancer Cells Grown in 2D and 3D Formats|
Ekaterina Perminov, Jijun Hao
Evaluation of Wnt/ß-catenin and BMP pathway proteins and gene expression in NSCLC cell lines A549 and H460 in 2D and 3D cultures.
|Touching BACE. How Inhibiting β-Amyloid Production by Steric Hindrance with a Novel Immunotherapy Improves Memory in PDAPP Mice|
Charles Evans1, 2, Rhian Thomas1, Emma Kidd1, Mark Good2
The work presented in this poster aims to assess whether intracerebroventricular (ICV) administration of 2B3 alleviated age dependent memory deficits in PDAPP mice and to determine whether 2B3 altered APP metabolism and NMDA receptor phosphorylation ex vivo.
|Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation|
Lisa Mutschelknaus, Carsten Peters, Klaudia Winkler, Ramesh Yentrapalli, Theresa Heider, Michael J. Atkinson, Simone Moertl
Exosomes are important intercellular communicators in the acute radiation stress-response. These nanometer-sized extracellular vesicles confer protective signals to neighboring cells and therefore promote the tumorigenic and radioresistant phenotype of head and neck cancer cells.
|Multiplexed Cell- and Bead-based Assays in a No-wash Format for Biologics Screening and Characterization|
David Onley, Lucy Chammas, Diana Caracino and Paul G. Wylie
Here we present the results generated from multiplexed no-wash protocols using TTP Labtech’s mirrorball, a laser scanning cytometer.
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