|Measuring Antitumor Effect of c-Myr-Max heterodimerization inhibitor 100258-F4 on Ovarian Cancer Cells using Cellometer Imaging Cytometry|
Leo L. Chan, Jiandong Wang, Xiaoli Ma, Hannah M. Jones, Fang Song, Weiyuan Zhang, Victoria L. Bae-Jump, Chunxiao Zhou
The effect from the c-Mycinhibitor 100258-F4 was clearly observed from the G0/G1 cell cycle arrest and the increase in early and late apoptotic cell. The Cellometer method can measure fluorescent cell-based assays such as cell cycle, apoptosis, protein expression, autophagy and viability. The ability to export the data to FCS Express facilitates simple data analysis and reporting to generate results for publications.
|Quantification of Natural Killer Cell-Mediated Cytotixicity using Celigo Imaging Cytometry|
Leo L. Chan, Srinivas S. Somanchi, Kelsey Rosbach, Dean A. Lee
Time-course tracking of % lysis eliminates multiple controls & the effect of non-uniform cell seeding in the final cytotoxicity calculation. The # of cells used is less than the cells needed for Release assays & Flow Cytometry. Flow cytometry & Release assays require a seeding density of 100K target cells increasing the number of effector cells to the millions. The visual observation of ADCC or CDC on the images can be convincing to conclude the functionality of antibodies or complements.
|A rapid 3D tumor spheroid analysis method using the Celigo Imaging Cytometry|
Leo L. Chan, Scott Cribbes, Sarah Kessel, Olivier Dery, Catherine Swingler, Dmitry Kuksin, Tim Smith, Jean Qiu, Maria Vinci, Lisa Patterson, Sue Eccles
• Celigo Imaging Cytometer is a versatile and powerful tool for 3D tumorspheroid analysis
• Assays such as measuring optimal seeding density for forming tumorspheroids and viability measurement have been demonstrated
• Advanced assays such size measurement, growth inhibition, invasion into matrigel®, migration on to collagen and HUVEC, and tissue invasion that have been performed by manual microscope observation can now be easily quantified using the automated imaging cytometry method
|Scale up of Atorvastatin Delayed Release Nanoparticles for Treatment of Hyperlipidemia: Quality by Design (QbD) Approach|
Gite S. M., Mirani A. G., Patravale V.B.
The studies describes the implementation of QbD approach in the systematic development of optimized Atorvastatin calcium delayed release nanoparticles (ACDRNPs) employing simple, efficient and cost-effectual technique.
|Analytical Quality by Design (AQbD) for Developing a Validated High-Performance Thin Layer Densitometry Method for Estimating Mangiferin in Human Plasma |
Rajneet Kaur Khurana, Atul Jain, OP Katare, Bhupinder Singh
Analytical methods are highly vital at every stage of the product development starting from characterization of drug substance to its
estimation in dosage form, biological samples and stability studies. Development of analytical methods require complete understanding of the method variables to attain the superior method performance
|A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications|
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren
This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.
|THe AtSCL26 transcription factor controls cross-talk between GA and N root architecture in Arabidopsis thaliana roots|
Beatriz Lagunas, Anthony D. Carter, Dafyd Jenkins and Miriam Gifford
Phenotypic and molecular evidence supports the hypothesis that developmental program enabling nodule formation arose during evolution from a lateral root ‘blueprint’ pre-existing in all higher plants . We reasoned that analyzing Arabidopsis genes orthologous to regulators of nodulation could shed insight on control of lateral root development. This led us to the discovery that an Arabidopsis GRAS family transcription factor controls lateral root development under specific nitrogen conditions.
|LOHA Comprehensive Assay for Single Nucleotide Polymorphism, Copy Number Variants and Loss of Heterozygosity Using SureSelect Target Enrichment|
Kyeong Soo Jeong, Arjun Vadapalli, Ashutosh Ashutosh, Paula Costa, Brian Peter, Stephanie Fulmer-Smentek, Magnus Isaksson, Jayati Ghosh, Douglas Roberts, Holly Hogrefe
Here we describe a comprehensive assay that enables researchers to identify SNP, INDEL, CNV, and LOH using SureSelect target enrichment. This design can be employed as a standalone entity or in concert with other bait designs for SNP and INDEL detection. We also describe methods for data analysis and visualization.
|An examination of specific cellular organelle-targeting nanotags using combined 3D Raman and SERS imaging |
Katherine Lau, Sarah McAughtrie, Karen Faulds, Duncan Graham
We investigated the specific targeting of endoplasmic reticulum and trans-Golgi network in Chinese hamster ovarian cells using functionalised nanotags. The targeting was examined using the combined 3D SERS and Raman imaging method.