|Capillary Electrophoresis Coupled with Micro Free Flow Electrophoresis for High Speed Two Dimensional Separations of Biological Samples|
Alexander C. Johnson, Michael T. Bowser
We propose using micro free flow electrophoresis (µFFE) as a solution to the under sampling problem.
|Impact of Molecular Surface Charge on Electrical Impedance Spectroscopy Biosensing|
Y. Ram, T. Yoetz-Kopelman, A. Freeman and Y. Shacham-Diamand
Molecular surface charge was found to be the dominant parameter when monitoring protein binding events by Electrical Impedance Spectroscopy with a charged redox couple. A biosensing device was fabricated, and a physical model was derived to explain the results.
|Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry|
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.
In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.
|Droplet-on-Demand Platform for Biochemical Screening & Drug Discovery|
L.D. van Vliet1*, F. Gielen1, A. Sinha2, B.T. Koprowski3, J.B. Edel4, X.Niu5, A.J. deMello3, F. Hollfelder1, & J. Motschman2
To demonstrate droplet on demand applications towards study of biological entities encapsulated in nanoliter droplets. Interfacing a droplet on demand platform with microfluidic chips allows for merging and dilution of droplets. This feature is applied to encapsulate yeast cells (S. cerevisiae) and multicellular organisms (C. elegans).
|Analysis of Polymers, Additives and Contaminates in Medical Devices using Pyrolysis-GCMS|
Pyrolysis GC-MS is a standard technique used to analyze industrial polymers. This poster will show the use of multiple temperature ramps to analyze for additives and contaminants, in addition to polymer identification, in several medical devices.
|Long-term on-chip storage and release of liquid reagents for diagnostic lab-on-a-chip applications|
Daniel Czurratis*, Yvonne Beyl*, Sven Zinober*, Franz Lärmer*, Roland Zengerle**
We present a concept which enables on-chip storage of liquid reagents at room temperature and allows an easy implementation into pressure driven LoC devices.
|Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes|
Ian J. Horner, Jerod J. Hurst, Nadine D. Kraut, Alyssa A. Rook, Crystal M. Collado, G Ekin-Atilla Gokcumen, and Frank V. Bright
Several commercial multi-purpose solutions (MPS) products contain polyhexamethylene biguanide (PHMB) and/or polyquaternium-1 (PQ-1) as antimicrobial agents. In this poster we report the effects of PHMB and PQ-1 on small unilamellar vesicles (SUV) that we have designed to mimic the average human corneal epithelial cell membrane.
|Direct Targets Identification of a Bioactive Compound|
Sylvain Blanc, Paul Bradley, Marie-Edith Gourdel, Michael Cholay, Gisèle Guimèse, Mike Mckenzie, George Nasi, Jean-Christophe and Barbara Ruggiero
Identifying protein partners of a small bioactive molecule is of great
interest in many aspects of life sciences and specifically in the drug
discovery and development process cycle. It is a support to (i) decipher
the mechanism of action after for example a “High Content” screening,
(ii) study “off-target” effects, (iii) adjust therapeutic indications and
clinical regimens of a drug and (iv) consider drug repositioning.
|3D-Tissue/ Whole-blood Co-culture Models Combined with Multi-Analyte Profile (MAP) Analyses for In-vivo-like Immunopharmacology|
Stein GM, Joos T, Schmolz M
Human Organotypic Test (HOT) Systems aim at in-vivo like substance characterisation of all preparations meant to act on the human immune system.