Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Join | Sign in
Home>Resources>Posters

  Posters

Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2

Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.

More Information
Low Cost EIS Methodology to Characterize Microelectrode Arrays used in Biological Applications
C. A. Santos *, M. A. Flores*, O. Pilloni* , J. Mireles Jr**., A. Falcón ***, T. Fiordelisio ***, L. Oropeza-Ramos*

Diagnostics of electrochemical impedance in micro electrodes for biological potentials measurements is essential. A low cost EIS methodology to characterize microelectrode arrays in contact with cellular medium is presented, and results are validated by comparing them to a commercial system.

More Information
Micro array of Ag-AgCl electrodes for cellular stimulation and sensing
O. Pilloni*, M. A. Flores*, C. A. Santos*, A. Torres**, J. García**, H. González**, L. Oropeza-Ramos*.

Silver-Silver Chloride is investigated as a fabrication material of planar microelectrodes for sensing and stimulation of excitable cells. A theoretical design method is presented. The platform was tested for different excitable tissue confirming its feasibility.

More Information
Automated Method to Determine Infectious Dose (TCID50)
Andrea Murphy, PhD and Catherine Swingler, PhD

Settings for each cell type and virus can be saved and re-used to assure objective results. Additional information such as cell health, cell cycle status can be determined from the same plates using Nexcelom designed applications. Time course studies can be carried out by scanning plates over several days/weeks allowing for additional information than may be generated by carrying out end-point assays alone.

More Information
Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients
1Kettenhofen R, 1Duenbostell A, 2Niedereichholz T, 3D’Angelo JM, 4Horai H, 5Schwengberg S, 1Bohlen H, 6Licher T"

Introduction of selected, pure human cardiomyocytes derived from induced pluripotent stem cell (hiPSCM) into a calcium transient imaging high throughput screening (HTS) assay to assess cardiotoxicity and drug efficacies.

More Information
Developing Functional Monoclonal Antibodies for Beta-3 Adrenergic Receptor
Miao Tan and Helena Mancebo

Our preliminary data showed that several mAb clones specifically bound to the receptor while increasing the receptor function by acting as agonists.

More Information
Early Immunogenicity Risk Mitigation
Chloé Ackaert, Brecht Hoedemaekers, Sofie Pattijn and Philippe Stas

The ability of a drug candidate to induce an unwanted immune response can be a significant hurdle during drug development and a reason for product failures. Unwanted immunogenicity can cause loss of efficacy, altered pharmacodynamics and stability problems. .

More Information
Mimetix® Electrospun Scaffolds for 3D Culture of Upcyte® and Primary Hepatocytes
E. Heister, R. McKean (1) S. D. Ramachandran, B. Munst (2) B. Burkhardt, V. Haussling, A. Niissler (3)

This study investigates the Mimetix® electrospun scaffold, which consists of highly uniform electrospun PLLA microfibres supporting the three-dimensional growth of cells, as a cell-based assay system for upcyte® and primary hepatocytes.

More Information
Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In Functional Assays
Daniel Brown (1), Niklas Larsson (2), Ola Fjellström (3), Anders Johansson (3), Sara Lundqvist (2), Johan Brengdahl (2), and Richard J. Isaacs (1)

We describe the application of back-scattering interferometry (BSI) to the characterization of small molecule ligand binding to human GPR39 (a GPCR targeted for type-2 diabetes therapy) overexpressed in crude membrane fractions in free solution, including how BSI-derived affinity and functional assay-derived potency correlate for compounds of varying scaffolds.

More Information
<< 8 9 10 11 12 13 14 >>
Showing Results 101 - 110 of 1149
Scientific News
URI Researchers Invent Lab-On-Paper
New paper-based platform for conducting a wide range of complex medical diagnostics.
TSRI Scientists Find Clues to Cancer Drug Failure
A pair of studies show how the primary protein responsible for multidrug chemotherapy resistance changes shape.
MR Spectroscopy Shows Precancerous Breast Changes in Women with BRCA Gene
A technique that monitors biochemical changes in tissue could improve the management of women at risk of breast cancer.
Adults Only Really Catch Flu About Twice a Decade, Suggests Study
Researchers developed a mathematical model of how our immunity to flu changes over a lifetime as we encounter different strains of the virus.
Water In Smog May Reveal Pollution Sources
Vapor variations in SLC inversions tied to cars, furnaces.
New Nanodevice Defeats Drug Resistance
Tiny particles embedded in gel can turn off drug-resistance genes, then release cancer drugs.
Genetically Speaking, Mammals Are More Like Their Fathers
A first of its kind study shows that who we inherit genetic variants from – our mother or father – is crucial for the development of diseases and for research studies aimed at finding causes and potential treatments.
‘Stem Cell’ Test Could Identify Most Aggressive Breast Cancers
Testing breast cancer cells for how closely they resemble stem cells could identify women with the most aggressive disease, a new study suggests.
DNA Reveals Surprise Connections Between Britain and Europe 8,000 Years Ago
New evidence shows wheat reached Britain 2,000 years before the arrival of wheat farming.
Hookworm Genome Sequenced
UMMS sequences hookworm genome; may lead to new treatments for tropical disease.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters