|EU-OPENSCREEN - The European Research Infrastructure of Open Screening Platforms for Chemical Biology|
EU-OPENSCREEN (www.eu-openscreen.eu) is the largest emerging academic chemical biology research infrastructure initiative in Europe with the aim to collaboratively develop novel research tool compounds with external scientists. As a joint effort of national networks in 16 European countries, EU-OPENSCREEN offers access to high-throughput screening platforms, chemistry services, an open-access database, a large compound collection and an open-access database.
|Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve Crystallization|
Michel Terray, John Stenson
The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7.4 and Citrate buffer pH 4.1 were used, which revealed interesting and contrasting data
|Reducing Biases in Small RNA Sequencing|
Adam Morris Ph.D., Dawn Obermoeller, Masoud Toloue Ph.D.
Small RNA-Seq libraries utilizing randomized adapters demonstrated significantly less bias and more even coverage due to reductions in ligase bias.
|Amplicon Based 16S Ribosomal RNA Sequencing and Genus Identification|
*Jan Risinger, *Lindsey Renken, +Josh Hill, +Noushin Ghaffari, PhD, +Richard P. Metz, PhD, +Charles D. Johnson, PhD, *Masoud M. Toloue, Ph
Rapid determination of highly complex bacterial populations through targeted amplification of 16S rRNA V1 - V3 hypervariable regions can provide an accurate gauge of diversity at taxonomic hierarchies as low as the genus level.
|Fighting Blindness with 3D-NET "Drug Discovery & Development of Novel Eye Therapeutics"|
Pilar Ventosa-Andrés, Nils Ohnesorge, Yolanda Fernández, Yolanda Alvarez and Breandán Kennedy
3D-NET, “Drug Discovery & Development of Novel Eye Therapeutics”, is a new European research consortium of industry and academic partners focusing efforts to enhance the discovery and development of drugs targeting ocular pathologies that lead to blindness.
|Direct flow synthesis of H2O2 catalysed by palladium supported on sulfonated polystyrene resins|
Aurélie Plas, Eduard Dolušic and Steve Lanners
Development of a continuous microfluidic process for the production of hydrogen peroxide by direct synthesis with a minimalised ecological footprint.
|Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells|
Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.
|Pressure-Based Volume Measurement Technology for In-process Measurement of Microplate Contents|
John Thomas Bradshaw, Bill Gigante, Christoph Schwedes
Modern drug discovery and research labs are utilizing complex automation systems to assist in
high throughput screening of novel drug candidates. A large portion of these laboratories are
using ANSI-SBS-standard 96- and 384-well plates to achieve the necessary throughput. The volume
of sample transferred into and out of these plates can be critical to the success of assays or
validation of procedures.
|Environmental Studies Using the Tecan QC Kit and an Automated Liquid Handling System|
George Rodrigues, Emily Avis, Alf Price, Verena Gerber
The study illustrates the effect of two environmental factors (air temperature and humidity) on the volumetric performance of a Tecan Freedom EVO ® pipetting robot, equipped with a liquid LiHa (Liquid Handling Arm). Tecan recommends that the EVO be installed in a controlled environment. However, some users choose to operate in less controlled conditions.