|Automated Solid-Phase Extraction of Oraganochlorine Pesticides from Drinking Water|
Frans Schoustsen and Pranathi R Perati
A new method was developed to automate the capture and elution of Organochlorine pesticides from drinking water. The concentrated analytes were then analyzed by GC with Electron Capture Detection (ECD).
|GC-MS/MS Analysis of Pesticide Residue in Green Tea Extracted by QuEChERS with Acetonitrile as Final Solvent|
Cojocariu C., Morgan P., Silcock P., Godula M
QuEChERS involves an initial step when a few grams of the sample are extracted with acetonitrile followed by a clean-up step (with dispersive-SPE) used to remove, to a certain extent, unwanted matrix compound (such as pigments, sugars, organic acids). With QuEChERS, the final extract ends up with the pesticides in acetonitrile, which, being polar solvent, can be
problematic in GC-MS.
|Increasing Extraction Efficiency of Pesticides and Dioxins from Wet Samples Using a Novel Polymer During Accelerated Solvent Extraction|
Katerina Bousova, Pranathi Perati, Rahmat Ullah, Kannan Srinivasan
Thermo Scientific™ Dionex™ ASE™ Prep MAP is a proprietary polymer designed to remove moisture and increase extraction efficiencies from wet samples including soils, tissues and food products. This unique formulation allows moisture removal under a variety of ionic strength conditions and accelerated solvent extraction conditions.
|A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing|
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe
Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.
|Mutation Induction in Sucrose Synthase 1 to Study Cold Acclimation in Winter Wheat|
Rita Armoniene, Gintaras Brazauskas
Sucrose synthase (Ss) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose being one of the main enzymes of carbohydrate metabolism. The objectives of this study were: to create a TILLING population in winter wheat; to identify new alleles of Ss1 gene; to compare relative expression of Ss1 in leaves and crowns of mutant versus wild type plants during cold acclimation.
|Evolutionary Changes in Proneural Gene Expression - atonal and ASH in Daphnia magna|
Marleen Klann & Angelika Stollewerk
The presented in this poster investigates the morphological and molecular development of sensory organs in Daphnia magna.
|Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)|
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1
Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance
|Genetic progress in the Romanian triticale breeding program|
Ittu Gheorghe*, Saulescu Nicolae, Ittu Mariana, Mustatea Pompiliu and Marinciu Cristina
Since 1984 yield raised up to 43 kg / ha¹ and year¹ or 0.74 % /year¹ this progress being achieved mainly by improving fertility of spikes, plumpness of kernel, the test weight and introduction of short straw RhtB1b (Rht1) and Ddw1 (Hl) genes. In order to improve the yield stability, under predictions of global climatic changes, improvement of genetic diversity to powdery mildew, leaf and yellow rusts, virus and spike diseases and also for pre-harvest sprouting are still demanded.
|Automatic Characterization of Lipids Using Charge Remote Fragmentation Ions and Peaks Characteristic of Fatty Acid Fragmentation From MALDI MS/MS Data|
Ningombam Sanjib Meitei*(1); Arun Apte(2); Dietmar Waidelich(3); Fadi Abdi(4); Matthias Glueckmann(3)
Automated identification of lipids using CRF ions and high energy MS/MS fragment ions and novel software.