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An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis
Xiaoqin Cheng, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney

A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.

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Acoustophoretic microfluidic device for high throughput DNA sequencing
V.V Unnikuttan1, H.N Unni 1

Multiphysics modelling for acoustic standing wave technology combined with micro-technology which can be used for manipulation and concentration on typical Lab-on-Chip devices for DNA sequencing.

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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Shubha Jain, F. Azam, H.N.Unni

We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. It is observed that geometry of channel has significant impact on the mixing performance of channel and also mixing time. This mixer could be used for trapping, sequencing of micro and macro molecules such as cell, DNA and protein samples under flow condition

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Development of a Paper-Based Fluidic Device for Phosphorus Detection
Patricia K. Rusch and Kyle A. Cissell

This poster displays the successful beginning stages of an innovative way to detect phosphorus in standing bodies of water by minimizing the quantity of chemicals used, reducing the cost of analysis instrumentation, allowing the potential for real-time monitoring, and producing consistent and reliable results.

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Assessment of the efficiency of encapsulation of a fluorescent drug using Nanoparticle Tracking Analysis
Patrick Hole, Pierre Peotta, Roberto Santoliquido, Bob Carr

This poster outlines an example where the methodology of Nanoparticle Tracking Analysis (NTA) is used to characterise nanoparticles for drug delivery purposes.

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Effective Comparison of Yeast Extracts Using High Resolution GC and GCxGC-HRTOFMS
David E. Alonso and Joe Binkley

The Pegasus GC-HRT and GC-HRT 4D instruments are effective tools for the comprehensive analysis of yeast extracts.
• Enhanced chromatographic resolution of the HRT 4D system facilitated compound characterization by improving spectral similarity scores
• High quality data resulted in excellent spectral similarity scores when compared to large, well-established databases (NIST, Wiley)
• High resolution, accurate mass data was crucial for determination of fragment, molecular, and adduct

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Performance Characteristics of a Comprehensive Two=Dimensional Gas Chromatography-High Resolution Time-of=Flight Mass Spectrometry System (GCxGC=HRTOFMS) Utilizing Chemical Ionization
Jonelle Shiel, Mark Merrick, Scott Pugh, Matthew Soyk, and Viatcheslav Artaev

Combining comprehensive two-dimensional gas chromatography (GC×GC) with a High Resolution Time-of-Flight Mass Spectrometer and Chemical Ionization is a powerful tool for compound identification. Performance of the LECO Pegasus GC-HRT 4D using Chemical Ionization (CI) was demonstrated using Benzophenone and Diesel. GC×GC separation results in narrow peaks, therefore, a high speed detection system is necessary.

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Non-Target Analysis of Electronic Waste Samples from China
Jonathan D. Byer, Ed Sverko, Kurunthachalam Kannan, Qian Wu, Joe Binkley

GC×GC-HRT is a powerful tool for the comprehensive analysis and
chemical characterization of analytes in complex matrices. The combination of high resolution front-end separation with high
resolution time-of-flight mass spectrometry made possible the
identification of compounds previously unknown in these samples.

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Single Cell Whole Genome Amplification
GE Healthcare

The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses a technology for amplification of genomic DNA from a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed a method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase.

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Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Transporting Microscopic Cargo Between Human Cells
Scientists have developed a virus-inspired delivery system for material transport between cells.
Tissue Damage Is Key for Cell Reprogramming
Researchers have shown tissue damage is important for cells to return to an embryonic state for cell reprogramming.
Metabolite Promotes Cancer Cell Transformation
Researchers have identified a metabolite that promotes cancer cell transformation and colorectal cancer spread.
Improving Drug Production with Computer Model
A model has been developed that can be used to improve and accelerate the production of biotherapeutics, cancer drugs, and vaccines.
Bird Flu Confirmed in the Netherlands
An outbreak of H5 avian influenza was confirmed in the Flevoland province of the Netherlands.
Pasteurised Bacterium Reduces Obesity and Diabetes
Researchers have discovered that an intestinal bacterium provides a lasting effect on the intestinal barrier.
Turning Off Asthma Attacks
Researchers discover a critical cellular “off” switch for the inflammatory immune response that causes asthma attacks.
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