|DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population|
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1
This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.
|IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION|
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik
The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <
|Automated detection and control of controlled substances|
Joe Bradley, Ian Johns
Scitegrity, in conjunction with the Pistoia Alliance and a number of pharmaceutical companies, have developed Controlled Substances Squared to scan large chemical libraries (millions of compounds) and determine whether a compound is considered as controlled in a selected country.
|Apoplastic Venom Allergen‐like Proteins of Cyst Nematodes Modulate the Activation of Basal Plant Innate Immunity by Cell Surface Receptors|
Jose L. Lozano-Torresa, Ruud H.P. Wilbersa, SonjaWarmerdama, Anna Finkers-Tomczaka, Amalia Diaz-Granadosa, Casper C. van Schaika, Johannes Heldera, Jaap Bakkera,b, Aska Goversea,b, Arjen Schotsa and Geert Smanta,b
We conclude that parasitic nematodes most likely utilize VAPs to suppress the activation of defenses by immunogenic breakdown products in damaged host tissue.
|A pico-liter semi-automated screening method for morphological identification of microorganisms from environmental samples|
Ms. Charmi Chande, Dr. Ravindra Phadke
An economically feasible microfabrication protocol was developed for fabricating micro features used for bacterial analysis from environmental samples like soil and green sulfur bacteria sample. Organisms were classified in 11 morphotypes by using open source Image analysis software named CMEIAS and the standardized protocol was confirmed by viable count and pure culture study
|Busted! First Detection of Steroid Hormones in Pacific Walrus Bones|
Charapata P, Horstmann L, and Misarti N
The Pacific walrus (Odobenus rosmarus divergens) is a candidate species for the Endangered Species List because Arctic sea ice has declined significantly in the past decades. This project focuses on validating a method for extracting steroid hormones from archaeological, historical, and modern bone creating a baseline of hormone levels during different climate regimes.
|Population characterization of Brazilian isolates of Ceratocystis spp. using microsatellites|
Edson Luiz Furtado, Ana Carolina Firmino, Michael Mbenoun, Denise Nakada Nosaki, Ariska Van der Nest, Jolanda Roux, Irene Bernes, Mike Wingfield
The genus Ceratocystis includes several species of economically important plant pathogens and has a global distribution. In Brazil, species in the genus cause disease and death of hosts such as cacao, eucalypts and mango. This study aimed to characterize the population structure and diversity of isolates of Ceratocystis fimbriata sensu lato collected from diseased Eucalyptus species and to compare these to isolates from cacao, mango, teak, fig, rubber and atemoya.
|Geneious R8: A Powerful and Comprehensive Suite of Molecular Biology Tools
Christian Olsen, Kashef Qaadri, Richard Moir, Matt Kearse, Simon Buxton, Matthew Cheung, Hengjie Wang, Jonas Kuhn, Steven Stones-Havas, Chris Duran
Geneious R8: A powerful and comprehensive suite of molecular biology tools.
|Putative Genes Identified on Two Growth Conditions of G. boninense|
Jayanthi N, Abrizah O, Low ETL, O-Abdullah M, Hogan M, Cuomo CA, Desjardins C, Abdul Manaf MA, Rajinder S, Birren B and Ravigadevi S
Putative genes identified on two growth conditions of Ganoderma boninense.