|An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis|
Xiaoqin Cheng, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney
A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.
|Acoustophoretic microfluidic device for high throughput DNA sequencing|
V.V Unnikuttan1, H.N Unni 1
Multiphysics modelling for acoustic standing wave technology combined with micro-technology which can be used for manipulation and concentration on typical Lab-on-Chip devices for DNA sequencing.
|Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics|
Shubha Jain, F. Azam, H.N.Unni
We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. It is observed that geometry of channel has significant impact on the mixing performance of channel and also mixing time. This mixer could be used for trapping, sequencing of micro and macro molecules such as cell, DNA and protein samples under flow condition
|Development of a Paper-Based Fluidic Device for Phosphorus Detection|
Patricia K. Rusch and Kyle A. Cissell
This poster displays the successful beginning stages of an innovative way to detect phosphorus in standing bodies of water by minimizing the quantity of chemicals used, reducing the cost of analysis instrumentation, allowing the potential for real-time monitoring, and producing consistent and reliable results.
|Assessment of the efficiency of encapsulation of a fluorescent drug using Nanoparticle Tracking Analysis|
Patrick Hole, Pierre Peotta, Roberto Santoliquido, Bob Carr
This poster outlines an example where the methodology of Nanoparticle Tracking Analysis (NTA) is used to characterise nanoparticles for drug delivery purposes.
|Effective Comparison of Yeast Extracts Using High Resolution GC and GCxGC-HRTOFMS|
David E. Alonso and Joe Binkley
The Pegasus GC-HRT and GC-HRT 4D instruments are effective tools for the comprehensive analysis of yeast extracts.
• Enhanced chromatographic resolution of the HRT 4D system facilitated compound characterization by improving spectral similarity scores
• High quality data resulted in excellent spectral similarity scores when compared to large, well-established databases (NIST, Wiley)
• High resolution, accurate mass data was crucial for determination of fragment, molecular, and adduct
|Performance Characteristics of a Comprehensive Two=Dimensional Gas Chromatography-High Resolution Time-of=Flight Mass Spectrometry System (GCxGC=HRTOFMS) Utilizing Chemical Ionization|
Jonelle Shiel, Mark Merrick, Scott Pugh, Matthew Soyk, and Viatcheslav Artaev
Combining comprehensive two-dimensional gas chromatography (GC×GC) with a High Resolution Time-of-Flight Mass Spectrometer and Chemical Ionization is a powerful tool for compound identification. Performance of the LECO Pegasus GC-HRT 4D using Chemical Ionization (CI) was demonstrated using Benzophenone and Diesel. GC×GC separation results in narrow peaks, therefore, a high speed detection system is necessary.
|Non-Target Analysis of Electronic Waste Samples from China|
Jonathan D. Byer, Ed Sverko, Kurunthachalam Kannan, Qian Wu, Joe Binkley
GC×GC-HRT is a powerful tool for the comprehensive analysis and
chemical characterization of analytes in complex matrices. The combination of high resolution front-end separation with high
resolution time-of-flight mass spectrometry made possible the
identification of compounds previously unknown in these samples.
|Single Cell Whole Genome Amplification|
The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses a technology for amplification of genomic DNA from a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed a method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase.