|Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize|
Rodrigues, M.1, Chegão, A. 1, Costa, A. 1, Fernandes, C.1, Quedas, F. 2, de Andrade, E.1
We demonstrate that plant improvement by means of transgenesis causse alterations in the expression profiles of endogenous non-target genes.
|Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult|
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1
We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.
|Study Of The Active Bacterial Community In Two Membrane Bioreactors|
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).
|Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing|
Sabrina Shore, Jordana Henderson, Anton McCaffrey, Gerald Zon, Richard Hogrefe
We describe an optimized workflow which suppresses adapter dimers, works with low RNA input and eliminates the need for a gel purification step.
|Mortality after Motor Vehicle Accidents; A Forensic and Pathological Analysis from a Regional Collaboration|
Mr G Bessant, Mr N Misra, Mr J Hollingsworth
We have formed a collaborative approach to data collection between Merseyside major trauma collaboration, Merseyside police force, and the regional coroner's offices with the aim of identifying areas within the Haddon matrix for targeted local intervention.
|Characterization of Protein and Protein Aggregates using Temperature-controlled Hollow-Fiber Flow-FFF|
Trevor Havard, Florian Meier, Evelin Moldenhauer, Soheyl Tadjiki, Thorsten Klein
Reproducibilty Improvements in Field Flow Fractionation can be achieved on two fronts. Instrument design and control, the system used in for this poster is does not require flow controllers or switching valves and there for produces the same conditions in every case. Fractionater design, the design of the cartridges used in this poster maintain excellent conditions to maintain constant pressure at the membrane removing unwanted effects of sale relaxation above the membrane s
|Rapid Biosensor-Based Detection of Environmental Contamination by Petroleum Hydrocarbons, PAHs and Heavy Metals|
Nigel J Silman*, Jeremy G Thomas & Peter Riggs
In this study, we evaluated different freeze-drying excipients to stabilise the biosensor and remove the requirement to store bacteria at -20??C.
|Quantitative Analysis Of Pesticides In Quechers Extracts Using UPLC- and APGC- MS/MS |
Sara Stead, Simon Hird, Eimear McCall
In this study, we demonstrate sensitive, accurate and repeatable results for the analysis of pesticides in QuEChERS extracts of a selection of
commodities below the regulatory limits.
|Innovative technology that enables RNAi in difficult to transfect cells|
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake
Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.