|One Pot Method for the Simultaneous Synthesis and Anchoring of Organic Monolith Inside COC Microchip Channel|
Y. Ladner, A. Bruchet, V. Dugas, J. Randon, G. Crétier, K. Faure
Study shows monolith synthesis in COC microchip, including: the influence of BME concentration on performances; the separations of primary amines.
| Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)|
Niaz Mahmood, Salma Sultana Alam, Rifit Ara Najnin, Nurun Nahar Fancy, Haseena Khan.
Study aims include identification and characterisation of all NBS containing genes in jute using degenerate primers designed from the conserved NBS domain, by using two species of jute; susceptible and wild type resistant varieties.
|Effect of Liquid Handling Quality Control on Biological Assay Performance|
Nathaniel Hentz, Ph.D and Tanya Knaide
High quality assays are required in order to take forward potential drugs, post screening. Therefore the liquid handling must be accurate and precise, as demonstrated in this experiment.
|Nanoliter Volume Pin Tool Transfers as Measured by a Dual-Dye Absorbance Method|
Duong T. Chau; Patrick H. Cleveland, Ph.D.; John Thomas Bradshaw, Ph.D.
Increasing costs of chemical compounds and commonly used solvents has pushed high throughput screening labs towards lower working volumes, specifically in the nanoliter range. The ability to controllably dispense “known” nanoliter aliquots of samples is desired, which can readily be achieved using Pin Tools.
|Why Is My Assay Failing? An Approach to Assay Equipment Optimization|
Tanya R. Knaide, John Thomas Bradshaw, Kevin Khovananth, Keith Albert
Assays can produce unexpected or failing results for a multitude of reasons. Variability may be introduced at any point within the assay process.
|A Simple, Robust Automated Multiplexed Cell-Based Assay Process for the Assessment of Mitochondrial Dysfunction and Cytotoxicity|
Brad Larson, Peter Banks, Tracy Worzella, Andrew Niles and Timothy Moeller
Recent studies have shown that an increasing number of drugs no longer on the market have negative effects on mitochondrial function in key organs such as the liver and heart. Therefore it is increasingly important to monitor the effects of lead compounds on mitochondrial function in relevant cell systems. The ability to incorporate a simple, rapid, multiplexed, predictive assay can make the detection of potential toxic effects easier to perform early on in the drug discovery process.
|An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators|
Brad Larson, Bruce Sherf (INDIGO Biosciences), and Peter Banks
The Androgen Receptor (AR) is a member of the family of nuclear receptors responsive to steroid hormones. This poster aims to devise, validate and perform a preliminary automated HTS screening campaign to identify novel modulators of AR activity.
|Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility|
Brad Larson, Peter Banks , Nicolas Pierre, Stéphane Martinez, and Francois Degorce
Since the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells.
|Validation of an Automated Cell-Based Bioluminescent TNFa Blocker Bioassay|
Brad Larson, Tracy Worzella, Rich Moravec, Neal Cosby, Frank Fan, Teresa Surowy and Peter Banks
TNFa blocker biopharmaceuticals represent an important and successful class of protein drugs used in the treatment of several autoimmune diseases. Bioassays are indispensible tools in biopharmaceutical drug development and commercialization that are used to quantify biological activity and stability of drugs or drug candidates. The automation of these assays can serve to create an accurate, robust process which can allow the researcher to perform other more important functions.