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Stable N-phosphorylated triazol-5-ylidenes
Kirilchuk,A. A.; Marchenko,A. P.; Koidan, H. G.; Zarudnitskii, E. V.; Huryeva, A. N.; Yurchenko, A. A.; Kostyuk, A. N.

Considering probable generality of “carbene” mechanism for 1,3-azoles, investigation of intermediates of phosphorylation of 1,2,4-triazoles is of interest.

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Reaxys Xcelerate – Less Searching. More Discovery
Keshav Dutt Sharma

Reaxys Xcelerate is a new premium version of Reaxys. Reaxys is a web based workflow tool for synthetic chemists. It is designed to support the optimization of synthetic processes and offers a valuable resource for accurate & validated experimental reaction & substance data.

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Automated Genomic DNA QC Ensures High Quality Data from Downstream Workflows
Arunkumar Padmanaban, Ruediger Salowsky, Delphine Rabiller and Donna McDade Walker

The success of several genomics study depends primarily on the quality of starting material, which in most cases is the genomic DNA. The quality and quantity of the extracted genomic DNA affects the downstream applications like microarray studies, library constructions and gene expression studies.

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High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam Inche

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

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FDG PET/CT Utility in Gynecologic Malignancies: A comprehensive review of anatomy, pathways of metastatic spread and scan findings
Nicholas Plaxton, MD, Aruna Polsani, MD, Raghuveer Halkar, MD, Karen Godette, MD, Barron, Bruce, MD

Our objective was to review the five major gynaecologic cancers (cervical, ovarian, uterine, vaginal, and vulvar) and demonstrate the role of FDG PET/CT in diagnosis, surveillance, FIGO staging and treatment strategy.

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FDG PET/CT Characteristics of Adrenal Benign and Malignant Lesions
Nicholas Plaxton, MD, Raghuveer Halkar, MD, Bruce Barron, MD

We selected FDG PET/CT cases with strong key representative findings to help illustrate benign and malignant adrenal lesions. Tabular review of PET SUV values, Hounsfield units and lesion size in the different cases will be discussed.

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Hot Start dNTPs – Pushing the Limits of PCR
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

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RNA Quality Control using the Agilent 2200 TapeStation System –Assessment of the RINe Quality Metric
Arunkumar Padmanaban, Ruediger Salowsky, Charmian Cher

Here, we present a comparative study between the RINe quality score obtained from R6K ScreenTape and High Sensitivity R6K ScreenTape compared to the RIN quality metric obtained from the 2100 Bioanalyzer system.

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Variation in miRNA expression between TRV-infected tobacco plants is correlated with symptom severity and TRV-16K transcripts abundance
Zhimin Yin, Krystyna Michalak, Miroslawa Chrzanowska

In the present work, expression of certain miRNAs and their targets were investigated in three tobacco plants infected with Tobacco rattle virus (TRV) isolate Deb57 collected from Northern Poland in 2008.

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Scientific News
Early Treatment Benefits Infants with Severe Combined Immunodeficiency
NIH-funded study identifies factors contributing to successful stem cell transplants.
Stem Cells from Nerves Forming Teeth
Findings published in the scientific journal Nature.
UTSW Cancer Researchers Identify Irreversible Inhibitor for KRAS Gene Mutation
Irreversible inhibitor for KRAS gene mutation involved in lung, colon, and pancreatic cancers.
NIH Scientists Find Six New Genetic Risk Factors for Parkinson’s
Study shows power of combining big data analysis with cutting-edge genomic techniques.
UT Southwestern Researchers Uncover New Brain Pathways
New therapies for treating Type 2 diabetes and obesity.
Mount Sinai Scientists and International Team Shed New Light on Schizophrenia
Genes and pathways identified could inform new approaches to treatment and address acute need for drug development for this disorder.
Mass Spec-Based Metabolomics Workflows to Evaluate Plant Immunity
This article reviews metabolomics studies used to evaluate plant immunity with an emphasis on mass spectrometry in its many forms.
Schizophrenia’s Genetic Skyline Rising
Suspect common variants soar from 30 to 108 - NIH-funded study.
UK Government Must Do More to Reduce Risk of vCJD Infection
Report recommends that UK Government commission a full assessment of the risks currently faced by the UK blood supply.
Future Directions of Forensic DNA Databases
With the great success of the use of forensic DNA databases, new challenges are emerging.
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