|Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis|
Wendy Goodrich, Peter Banks, Ron Osmond, Antony Sheehan
Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states. Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states.
|Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup|
Wendy Goodrich, Jason Greene, Mary Louise Shane
The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.
|Improving Cell-Mediated Cytotoxicity Assessment through the Use of an Automated Luminescent ADCC Assay|
Brad Larson, Sumant Dhawan, Shalini Wadwani, and Peter Banks
Assays that can assess the ability of a biosimilar to act in a manner similar to the original biologic have seen increased interest. This poster describes the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality.
|The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation|
Mosca, RC., Isaac, C., Mathor, MB
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
|Modular Bench Top Automation|
Paul Held, Dean Mulyk, Lenore Buehrer and Grace Mangialardi
ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.
|Screening for Optimal Algal Cell Growth and Neutral Lipid Production Conditions in Microplates|
Paul Held, Xavier Amouretti, Wayne Patton and Peter Banks
Algae based products offer many potential advantages over food based sources of energy, however significant research is still required in order to make them an economically viable option. To date, algal research has not extensively utilized the tool of high throughput screening using microplates that has been successfully employed in fields such as drug discovery.
|Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells|
T Anand, D Kumar, M S Chauhan, and P Palta
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
|Antigen Determination in Autoimmune Hepatitis Type1|
Naveen L Gupta, S Nayak, S Shakeyavar
Objectives of this project were to exploit the database in indian setting to determine nuclear antigens as target for antinuclear antibodies (ANA) in patients of autoimmune hapatitis (AIH) type1.
|Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos|
D Kumar, T Anand, P Palta and M.S. Chauhan
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.