|Rapid Identification of Microorganisms by Touch Spray and Paper Spray Ambient Ionization|
Ahmed M. Hamid, Alan K. Jarmusch, Valentina Pirro and R. Graham Cooks
The rapid and accurate identification of microorganisms is necessary in order to improve public health. Molecular-based identification allows for rapid patient treatment and provides more accurate diagnoses, two crucial aspects for successful treatment, yet a great challenge. In this work PS-MS, an existing ambient method, and TS-MS, a newly developed technique, are being utilized in the rapid discrimination of microorganisms (<2 min).
|Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system|
Anne F Hammerstein, Diana Caracino, and Paul Wylie
The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.
|Combining low and high volume liquid handling capabilities for ADME screening|
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
|Inverted Y Approach to Lumbosacral region - Analysis of 525 cases over 10 years|
Dr Krishna Kumar and Dr Renjith Kumar
Traditional approach to lumosacral region utilizes midline incision, which often requires long incision to get sufficient exposure. We describe our experience with inverted Y approach to lumbosacral junction.
|Alternative Cellular Energy Based Therapy of Childhood Diarrhea|
Rafael R. Izaguirre, Miguel R. Guzman, Rafael C. Fuentes, Carlos E. Mena, Emillio Penate, W John Martin
Acute infectious diarrhea is a major cause of morbidity and mortality in the developing world; especially among children less than 5 years of age. As restoring normal cellular function is an energy-requiring process, patients with diarrhea could potentially benefit from receiving an alternative (non-mitochondria) source of cellular energy.
|Effect of cinnamomum tamala leaves extracts on collagen cross-linking and tensile strength in wound healing in diabetic rats|
Rupesh Soni, Amit Vairagi and D. N. Srivastava
Diabetes can result in development of several complications, including delayed wound healing. Diabetic wounds are slow, non-healing wounds that can persist for weeks despite adequate and appropriate care.
|Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?|
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild
Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.
|A Distinctive Analysis of Fluid Flow Behaviour in an AC Electroosmotic Micropump|
Nurul Amziah Md Yunus, Farideh Abhari, Mohd Nizar Hamidon, Zurina Zainal Abidin, Izhal Abdul Halin, Gerard H. Markx and Nicolas G. Green
Microfluidic systems are used for chemical and biological analyses, sensing, drug delivery and molecular separation.
|Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies|
W. John Martin
There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.