|Molecular dynamics of POPC and POPE lipid membrane bilayers enforced by an intercalated single-wall carbon nanotube|
Sergey Shityakov, Thomas Dandekar
In this study, we used high temperature molecular dynamics simulation (400 K), because it is known that critical fluctuations of lipid membranes can even occur at 313 K and especially above 343 K the interlamellar water layer thickness starts to increase non-linearly due to "hydration force". We implemented this method to emphasize POPC and POPE (palmitoyl-oleoyl-phosphatidylethanolamine) membrane bilayer stability enhanced with single-wall carbon nanotube.
|Three-dimensional quantitative structure-activity relationship analysis and ADME predictions of guanylhydrazone coactivator binding inhibitors of estrogen receptors|
Sergey Shityakov, Thomas Dandekar
The estrogen receptors (ER) refer to a group of the nuclear hormone receptor superfamily of ligand-mediated transcriptional factors. Over expression of this type of receptors leads to a breast cancer progression. Hormone-responsive breast cancer develops resistance to conventional anti-cancer therapy, and this becomes a major problem in a breast cancer therapy.
|Attempts of facilitated DelF508-CFTR trafficking to the plasma membrane|
Sergey Shityakov, Massimo Micaroni, Alexander A. Mironov, Alberto Luini
Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations of the CF transmembrane conductance regulator protein (CFTR), a cAMP-regulated chloride channel. One of the most common CFTR mutations is the deletion of phenylalanine in 508 position (DelF508-CFTR). This mutation induces small conformational change hence the CFTR trafficking is no more effective. The main idea is to find a molecule to facilitate the DelF508-CFTR trafficking to the plasma membrane.
|Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis|
Joanne E. Lee and John F. Golz
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
|Novel Serological Assay Using HIgh-Throughput Chromogenic Microarray Technology for Screening SPF Non-human Primates|
Justin Brink, Audra Hachey, Keith Mansfield, Kim Luke
Routine surveillance programs to detect microbial agents in non-human primate colonies are critical to maintaining colony health and safety, as well as research integrity. Serological testing is a crucial element of maintaining colony health, but current methods rely on slow, expensive, and low-throughput ELISA-based serological assays. Here we describe the development of a sensitive, specific, robust, and easily automated method for colony health screening programs.
|Streamlined QbD HPLC Method Development with Automated Data Processing|
Gang Xue1, Jeff Harwood1, Charles Cheng1, David Fortin1, Jian Wang1, George Reid1, Mike McBrien2, and Andrey Vazhentsev2
Quality by Design (QbD) has been widely applied to pharmaceutical manufacturing to enhance process robustness, and improve product quality and manufacturing productivity. Recently, there arose tremendous interests from both the pharmaceutical industry and regulatory agents to expand it to analytical methodology.
|Drought Induced Increase in Quercetin Glycosides is Associated with Drought Resistance in a Novel White Clover Population|
Wouter L. Ballizany, Rainer W. Hofmann, Brent A. Barrett, Zulfi Jahufer
White clover is an important forage legume in temperate pastures, but does not have sufficient resistance against drought stress. Quercetin is a flavonol conferring plant sunscreen and anti-oxidant properties under UV-B stress with a trade-off of reduced biomass. An F1 full-sib cross between stress tolerant and stress sensitive white clover genotypes showed the ability to increase Q accumulation associated to maintenance of dry matter yield under a near wilting drought.
|Biopharmaceuticals Hiding in the Woods|
Prof. Rodney Savidge
Suggestions are provided of how efficient industrial bioprocessing can be accomplished, independently of highly expensive molecular biology approaches, in order to recover natural products of medicinal value from wild plant species.
|Characterizing Small Molecules in Biological Extracts using IntelliXtract Data Processing on High Resolution Accurate Mass Time-of-Flight Data|
Margaret Antler, Graham A. McGibbon, Vitaly Lashin, Jeffrey S. Patick and Kevin Siek
To detect small molecules in biological extracts, LC/MS performed using high resolution time of flight with accurate mass analysis is a powerful technology. Together with advanced data processing algorithms, accurate mass and relative isotope abundance measurements, molecular formulae can be determined and compounds identified.