|AUTOMATED WORKFLOW FOR THE DETERMINATION OF FATTY ACID METHYL ESTERS (FAME)|
Beat Schilling1, Reto Bolliger2, Guenter Boehm2
Transesterification of fatty acid esters with Na-methoxide is a fast, efficient and very robust method for fat analysis in food samples. With the use of three ISs the completeness of the transesterification as well as the extent of undesired saponification can be checked.
The PALworkstation allows to fully automate the FAME preparation, including injection into the GC.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
|New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples|
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji
We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.
|Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies|
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom
Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.
|Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer|
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT
SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.
|Characterization of Antibody-Drug Conjugates by SEC with Combined Light Scattering |
Chris C. Broomell
Drug Antibody Ratio (DAR) is a critical factor to the effective design of antibody-drug conjugates (ADC). This poster presents a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.
|High-throughput analysis of protein formulations by DLS|
Sophia Kenrick and Daniel Some
High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1
|GC-MS Comparison of Lavindin Grosso Oil Obtained by Steam Distillation and SFE|
Claudia Worley, Lisa Zona, Susan Sonchik Marine
Oil was extracted from Lavindin Grosso using steam distillation and using supercritical fluid extraction (SFE). Multiple analyses of the same extract demonstrated repeatability of the analytical method. The variability between batches of distillate bracketed the composition obtained by SFE. The differences among the oils could be attributed more to where the lavender was grown than to the extraction method.
|Digital microphotographic atlas of the adult zebrafish cerebellum|
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.