|Transforming Analytical Data into Knowledge with Software for Metabolite Studies|
Tara Sinclair, Graham A. McGibbon and Susan Ling
ACD/Labs has been developing specialized software for chemical and pharmaceutical research for over 16 years. Their expertise includes multi-technique, multi-instrument data handling, knowledge management of analytical and separations data, chemical drawing, and prediction software for a range of chemical properties as well as spectra and chromatograms.
|Repurposing Drugs for the Treatment of Multi-Drug Resistant Breast Cance|
David Monaghan, Rachel Griffin, Amie Regan, Enda O’Connell, Howard Fearnhead
In this study, the Johns Hopkins Clinical Compound Library, containing approximately 1,500 FDA and foreign-approved clinical compounds, was used to screen a multi-drug resistant, triple negative breast cancer cell line for drug sensitivity.
|Production of Naturally Compressed Screening Arrays|
Steven A Trim.
Animal venoms and toxins are a rich source of novel biologics with several making the progression from tool to therapeutic such as FDA approved Integrilintm (Eptifibatide) (Millennium pharmaceuticals)1 derived from Rattlesnake venom for unstable angina.
|Optical Microchip Sensors for Multiplexed Detection of Biological Pathogens|
D. Bhatta, A. Michel, M. Marti Villalba, G. D. Emmerson, I. J. G Sparrow, M. B. McDonnell, E. A. Perkins , R. W. Ely and G. A. Cartwright
SpectroSens, a multi-channel optical microchip sensor system suitable for rapid, label-free multiplexed detection of a wide range of bio-hazardous agents is presented. Optical chips containing multiple high-precision planar Bragg gratings are exploited as low-cost, robust refractive index sensors.
|Low cost direct optical structuring of lab-on-a-chip devices using stamps photopolymer|
German Comina, Jose Solis, Walter Estrada
In this work the development of microfluidics devices using a simple, fast and low cost fabrication method is shown. The devices were made using a photopolymer designed for making stamps. The obtained results make this concept a convenient technology for configuring compact service areas of specialized microfluidic and detection microstructures compatible with autonomous LOC devices.
|Intronic polymorphisms in Daucus carota AOX2b generate putative genotype specific miRNA|
Hélia G. Cardoso, Maria Doroteia Campos, Birgit Arnholdt-Schmitt
A study in the carrot alternative oxidase gene DcAOX2b from several individual plant genotypes of D. carota cv. Rotin revealed the frequent occurrence of intron length polymorphisms (ILPs). Here we will present an in silico analysis performed in order to identify putative miRNA sites at three different sizes of intron 1. The overall research approach aims to develop functional marker candidates for carrot plant breeding.
|A fast and fully automated solution for Lipidic Cubic Phase (LCP) screening using mosquito LCP|
Joby Jenkins1, Patricia Edwards2, Rob Lewis1and Joanne Franklin1
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
|Expansion of mesenchymal stem cells from frozen UCB|
Christophe NP Madsen and Christian Clausen
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
|A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3|
Andrew Goulter and Jason Mundin
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.