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Microextraction techniques combined with X-ray fluorescence spectrometry for determination of different metal ions in water
Karina Kocot (a), Beata Zawisza (a), Eva Marguí (b), Ignasi Queralt (c), Rafal Sitko (a)

In present work new preconcentration procedures combining microextraction techniques and X-ray fluorescence spectroscopy were developed. Proposed methodologies are promising tools for multielemental analysis enabling detection of natural pollutants in different water samples.

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HOMOGENEOUS ADCs BEARING TWO DIFFERENT PAYLOADS SHOW STRONG SYNERGY IN TUMOR KILLING
Lisha Allen, Yi Mi, Muhammad Abbas, Wolfgang Richter, and Sean Hu

We presented a one-pot reaction to prepare site-specific ADCs using available mABs on hand without re-engineering. We have identified a novel non-cleavable linker, which increases not only ADC stability, but also potency. We also demonstrate synergy in cancer cell killing of hybrid ADC loaded with two different toxins

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Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.

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Toward a Comprehensive Bottom-up and Top-down Analysis of the NIST Reference Monoclonal Antibody
Chris Becker1, Eric Carlson1, Wilfred Tang1, Yong Kil1, Marshall Bern1, John Schiel2, Lisa Kilpatrick2, Trina Formolo2

Development of ADC and other therapeutic proteins require careful characterization of sequence, post-translational modifications, sequence variants, and degradants. In this study, a NIST mAb interim reference material was analyzed by new bioinformatics tools (Byonic™, Byologic®, and Byomap™) allowing one to efficiently identify and quantify modifications down to trace levels.

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Application of Central Composite Design (CCD) for the Optimization of Ultrasound-assisted Extraction (UAE) of Phenolics from Corn (Zea mays)
Widiastuti Setyaningsih, 1,2 Erwan Duros, 3 Ceferino Carrera, 4 Miguel Palma, 2 Carmelo G. Barroso 2

A new Ultrasound-assisted Extraction (UAE) of phenolic compounds from corn (Zea mays) has been optimized by means of chemometrics tool. The proposed method was validated and successfully applied to the analysis of phenolics content in several corn cultivars.

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Metabolism of Eight Model Pharmaceutical Compounds in Rat and Human HepatoPac Versus Liver Microsomes and Suspension Hepatocyte Platforms
Julius O. Enoru, William DeMaio, Adiba Watanyar, Kenneth Draper, Amanda Moore & Okey Ukairo

This poster presents our comparative metabolite profiling analysis of eight model pharmaceutical compounds with various biotransformation reactions using a functionally stable model of primary hepatocytes [micropatterned co-cultures (MPCCs)] in parallel with the traditional liver microsomal and suspension hepatocyte systems.

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Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)

We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.

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High Titre BacMam viruses improve transduction efficiency of mammalian cells
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.

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Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke

Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.

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Showing Results 151 - 160 of 1238
Scientific News
Starting Antiretroviral Treatment Early Improves Outcomes for HIV-infected Individuals
NIH-funded trial results likely will impact global treatment guidelines.
Scientists are Designing Decoy Drugs to Fool Cancer
Study shows potential of drugs that can target the Notch switch with reduced toxicity.
Viral Immunotherapy For Skin Cancer Shows Patient Benefit
A genetically engineered herpes virus can halt the progression of skin cancer by killing cancer cells and sparking the immune system into action against tumours, a landmark clinical trial has shown.
Seeing the Action
Researchers have developed a way to observe both the forces present and the behavior that occurs during cell hemifusion; the merging of the lipid bi-layers of two cells.
Non-Aqueous Solvent Supports DNA Nanotech
Researchers at the Georgia Institute of Technology have now shown that they can assemble DNA nano-structures in a solvent containing no water.
‘Rosetta Stone’ for Prostate Cancer Mutations
Scientists have produced a comprehensive genetic map of the mutations in prostate cancer that have spread across the body.
From Worker to Queen at the Drop of a Gene
Biologists have found that the hierarchical fate of one of nature's most important pollinators comes down to which genes are "turned on or off".
First Step Towards Global Attack on Potato Blight
European researchers and companies concerned with the potato disease phytophthora will work more closely with parties in other parts of the world.
Bioresorbable Electronic Stent Could Provide Feedback, Therapy
Researchers have developed and tested a drug releasing electronic stent which could significantly reduce the risk associated with traditional mesh tube stents.
Tiny Heart, Big Promise
Researchers have discovered a new source of cells that can develop into coronary vessels.
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