|System-Specific Periodicity in qPCR Data and its Impact on Quantitation|
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3
In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.
|Bovine RNA-seq data analysis of liver and pituitary gland|
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.
Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.
|A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction|
Juan Triviño Paredes1, Atilla Biçer1, Arnauld Besson2, Edurne Gallastegui1, Josep Maria Estanyol3, Maria Jesus Pujol1 and Oriol Bachs1
A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction
|Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult|
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1
We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.
|Study Of The Active Bacterial Community In Two Membrane Bioreactors|
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).
|Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing|
Sabrina Shore, Jordana Henderson, Anton McCaffrey, Gerald Zon, Richard Hogrefe
We describe an optimized workflow which suppresses adapter dimers, works with low RNA input and eliminates the need for a gel purification step.
|Rapid Biosensor-Based Detection of Environmental Contamination by Petroleum Hydrocarbons, PAHs and Heavy Metals|
Nigel J Silman*, Jeremy G Thomas & Peter Riggs
In this study, we evaluated different freeze-drying excipients to stabilise the biosensor and remove the requirement to store bacteria at -20??C.
|Quantitative Analysis Of Pesticides In Quechers Extracts Using UPLC- and APGC- MS/MS |
Sara Stead, Simon Hird, Eimear McCall
In this study, we demonstrate sensitive, accurate and repeatable results for the analysis of pesticides in QuEChERS extracts of a selection of
commodities below the regulatory limits.
|Characterization of Protein and Protein Aggregates using Temperature-controlled Hollow-Fiber Flow-FFF|
Trevor Havard, Florian Meier, Evelin Moldenhauer, Soheyl Tadjiki, Thorsten Klein
Reproducibilty Improvements in Field Flow Fractionation can be achieved on two fronts. Instrument design and control, the system used in for this poster is does not require flow controllers or switching valves and there for produces the same conditions in every case. Fractionater design, the design of the cartridges used in this poster maintain excellent conditions to maintain constant pressure at the membrane removing unwanted effects of sale relaxation above the membrane s
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