|Quantitative Cell-Based Bioassays for Individual and Combination Immune Checkpoint Immunotherapy Targets|
Zhi-jie Jey Cheng, Jamison Grailer, Pete Stecha, Jun Wang, Jim Hartnett, Frank Fan, and Mei Cong
Immune checkpoint receptors are promising new immunotherapy
targets for the treatment of a variety of diseases including cancer and
autoimmune-mediated disorders. We developed a suite of cell-based
bioluminescent reporter bioassays for individual and combination
immune checkpoint immunotherapy targets including: PD-1 (PD-L1 or PD-L2), CTLA-4, LAG-3, TIGIT, PD-1+TIGIT, GITR, 4-1BB, CD40, and OX40.
|A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources|
Mira Genser-Nir, Sharon Daniliuc, Marina Tevrovsky, Roni Hazan Brill, Yuliya-Yael Miropolski, David Fiorentini.
An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications
|A Synthetic CRISPR-Cas9 System for Homology-directed Repair|
John A. Schiel, Maren M. Gross, Emily M. Anderson*, Eldon T. Chou, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Synthetic, dual-RNA-encoded Cas9 is used for precise homology-directed repair (HDR) gene engineering. Both short and long (GFP) inserts are covered.
|Phenotypic Cell-Based Screening of a High Content Imaging Cell Health Assay using the IN Cell Analyzer 2000|
Zaynab Neetoo-Isseljee, Chido Mpamhanga, David Tickle, Debra Taylor and Janet Brownlees
A High Content Imaging Cell Health assay has been established in-house for phenotypic High Content Screening using the IN Cell Analyzer 2000 and Genedata Screener analysis software. We describe the assay development and initial screening of the FDA set in HepG2 cells for validation of the assay.
|CRISPR-Cas9 genome editing utilizing chemically synthesized RNA|
Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
CRISPR-Cas9 gene editing using synthetic crRNA:tracrRNA or sgRNA is highly efficient and easy to use. Synthetic crRNA:tracrRNA is uniquely suited to in vitro and in vivo applications, in particular, DNA-free approach with Cas9 mRNA. Chemical synthesis of guide RNAs allows accurate and rapid production of arrayed crRNA libraries for high-confidence, loss-of-function screens.
|Deep Phenotyping - Harnessing Data Richness for Unsupervised High-Content Analysis|
Huang Dong, Wang Yi, Maciej Hermanowicz, Ke Yiping, Maja Choma, Lee Kee Khoon, Frederic Bard
Recognising the key challenges, we develop an end-to-end computational framework for HCA dubbed “Deep Phenotyping” that perform unsupervised analysis to leverage on the data richness for the discovery of unknown sub-phenotypes with minimal labeling cost.
|Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay|
Stefanie Buesch1 , John Langer2 , Sabine Schaepermeier1, Lubna Hussain2, Jeffrey Bergeron3, Volker Vogel1, Jenny Schroeder1
This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay.
|Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System|
Cecile Villemant1 , Sabine Schäpermeier2 , Stefanie Büsch2 , John Langer3 , Theresa D’Souza3 , Lubna Hussain3 , Grant Cameron1 , Volker Vogel2 , Jenny Schroeder2
This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures.
|Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System|
John Langer1 , Jenny Schroeder2 , Lubna Hussain1 , Claudia Schwartz2 and Theresa D’Souza1
The RAFT™ 3D cell culture system provides a valuable tool to investigate different cell types singularly or in co-cultures in an in-vivo like collagen based microenvironment.
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