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Exploring The Benefits Of Automated Unattended Sample Derivatization Prior To Gas Chromatography Analysis
A. Caruso, M. Santoro, P. Magni, S. Pelagatti, R. Facchetti

Purpose: Exploring the possibility to perform the sample preparation steps necessary for the derivatization of analytes in a fully automated and reliable way by means of the same robotic sampler used for sample injection into the gas chromatograph.
Methods: As an example of application we screened dairy products for melamine and its derivates presence via GC-MS/MS.
Results: The outcome are good and precise results that show how it is possible to reliably automate sample preparation opera

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Determination of Veterinary Drug Residues in Milk by GC/MS and GC/ECD
Yu Chongtian, Lv Jianxia

Purpose: Develop a rapid, sensitive method for determining amitraz and its metabolites in milk.
Methods: Samples were hydrolyzed in acidic condition and extracted with hexane. After derivativation, the samples were analyzed by GC-ECD or directly by GC-MS.
Results: The results show that GC-MS determination of amitraz and its metabolites is rapid and sensitive.

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Automated, Rapid And Reliable Determination Of Dissolved Gases In Water By Static Headspace – Gas Chromatography
A. Caruso, M. Santoro, F. Bedini, P. Magni, S. Pelagatti

Purpose: Exploring the possibility to perform a quantitative determination of dissolved gases in water in a reliable and accurate way. Methods: The quantification of dissolved gases is performed by building a calibration curve spiking blank water samples with increasing quantities of saturated water followed by headspace sampling and GC/ FID analysis. Results: The results show good correlation factors and limit of quantification lower than 10ppb for the four gas analyzed.

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Coping with BIG DATA Image Formats: Integration of CBF, NeXus and HDF5, A Progress Report
Herbert J. Bernstein,[1] Jonathan M. Sloan,[2] Graeme Winter,[2] Tobias S. Richter,[2] NeXus International Advisory Committee,[3] Committee on the Maintenance of the CIF Standard[4]

The best of CBF/imgCIF (the Crystallographic Binary File, which has a complete description of the experiment), NeXus (a common data framework for neutron, X-ray and muon science, which gracefully handles large data sets) and HDF5 (Hierarchical Data Format, version 5, the high-performance data format used by NeXus) are being combined for the management of BIG DATA at synchrotrons.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.

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Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom

Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.

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Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT

SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.

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Characterization of Antibody-Drug Conjugates by SEC with Combined Light Scattering
Chris C. Broomell

Drug Antibody Ratio (DAR) is a critical factor to the effective design of antibody-drug conjugates (ADC). This poster presents a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.

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Showing Results 31 - 40 of 1004
Scientific News
Detecting and Identifying Candida Species in Blood Samples of Critically Ill Paediatric Patients
The study aimed to develop a multiplex nested PCR method to detect and identify seven Candida species in peripheral blood samples of critically ill paediatric patients.
Cancer-Fighting Drugs Might Also Stop Malaria Early
A number of compounds have been identified which could be used to fight malaria.
MU Researchers Discover Protein's Ability To Inhibit HIV Release
TIM-family proteins have the ability to block the release of HIV and other viruses.
How to Prevent Organic Food Fraud
A new test under development has the potential to authenticate organic tomatoes and other produce.
Bowel Disease Gene Discovery
Genetic changes that occur in patients with the bowel condition Crohn’s disease could hold clues to fighting the illness.
Evolutionary History of Honeybees Revealed by Genomics
Researchers found that glaciation and historic climate change has significantly impacted honeybee populations.
NIH Announces the Launch of 3 Integrated Precision Medicine Trials
ALCHEMIST is for patients with certain types of early-stage lung cancer.
Cancer Leaves a Common Fingerprint on DNA
Chemical alterations to genes appear key to tumor development.
Monthly Blood Transfusions Reduce Sickle Cell Anemia-related Brain Injury in Children
NIH-funded study provides hope for children with disease-related brain damage.
Researchers Obtain Key Insights into How the Internal Body Clock is Tuned
New way to regulate internal body clocks by long non-coding RNA.
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