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Hot Start dNTPs – Pushing the Limits of PCR
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

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RNA Quality Control using the Agilent 2200 TapeStation System –Assessment of the RINe Quality Metric
Arunkumar Padmanaban, Ruediger Salowsky, Charmian Cher

Here, we present a comparative study between the RINe quality score obtained from R6K ScreenTape and High Sensitivity R6K ScreenTape compared to the RIN quality metric obtained from the 2100 Bioanalyzer system.

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Variation in miRNA expression between TRV-infected tobacco plants is correlated with symptom severity and TRV-16K transcripts abundance
Zhimin Yin, Krystyna Michalak, Miroslawa Chrzanowska

In the present work, expression of certain miRNAs and their targets were investigated in three tobacco plants infected with Tobacco rattle virus (TRV) isolate Deb57 collected from Northern Poland in 2008.

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Discovery of Conserved Domains for Development of Functional Markers and Identification of microRNAs from Drought Responsive Expressed Sequence Tags (ESTs) in Chickpea (Cicer arietinum L.)
Mandape M, Saikumar K, Dudhe MY, Kumar J, Meena HP, Chauhan J, Afroz A

This stidy identified that conserved domains or gene families from the ESTs could be utilized for the identification of gene-targeted markers and for development of functional markers to accelerate marker assisted backcross breeding in chickpea.

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A Crystallographic-Based Fragment Screen Against Human BRD4 Bromodomain 1
Hubbard P, Ritchie A, Hughes S, Lipkin M, Blackaby W & MacLeod A

Fragment-based drug discovery has now become firmly established as an important method in the lead discovery process, and is an integral component of drug discovery at BioFocus. Using a selection of compounds from our fragment collection, we have performed a crystallographic-based screen against human BRD4 bromodomain 1.

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Application of the SoftFocus® Design Strategy to Epigenetics Targets
Sheppard D, Macritchie J, Allen V & Hughes S

Epigenetics describes the regulation of gene expression that occurs through DNA methylation and post translational modification of histones. The pharmaceutical industry has shown an explosion of interest in this field with new targets emerging.

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Fragmentor: A New Technique to Facilitate Scaffold Design for Library Generation
Sheppard D, Macritchie J & Allen V

The requirement for novel starting points to ignite drug discovery projects is always high for emerging targets. Over the past 12 years BioFocus has built an impressive track record for the successful and original design of high quality libraries.

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Mohamad Al-shammari wins Poster Award at System Biology Europe 2012
Mohamad Al-Shammari

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012

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Determination and Comparison of Specifics of Nucleus Pulposus Cells of Human Intervertebral Disc in Alginate and Chitosan– Gelatin Scaffolds
Masoud Ghorbani, Hamid Bahramian Renani , Batool Hashemibeni Beni, Zeinab Karimi

In this work we studied specefices of NP cells obtained from nucleus pulposus by collagenase enzymatic hydrolysis obtained from patients undergoing open surgery.

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Showing Results 31 - 40 of 889
Scientific News
Nuvo Research Announces Scientific Presentation at the Annual Meeting of AAI
Data evaluating WF10's therapeutic impact on signs and symptoms of experimentally induced rheumatoid arthritis in mice.
An International Vision for Wheat Improvement
By 2050, a 60% increase in wheat production will be needed to meet the demand of a growing population.
Yale Cancer Center Carves New Path in Immunotherapy
Cancer immunotherapy is showing promise in treating patients with a variety of advanced, metastatic tumors.
UCSF Scientists Use Human Stem Cells to Generate Immune System in Mice
Raising hopes for cell-based therapies, UC San Francisco researchers have created the first functioning human thymus tissue from embryonic stem cells in the laboratory.
App Helps Doctors, Students Learn to Diagnose Neurological Symptoms and Disease
The new app that presents a novel approach to learning the neurological physical exam, a challenging series of assessments aimed at diagnosing neurological disorders in patients.
Preclinical Tests Shows Agent Stops “Slippery” Proteins from Binding, Causing Ewing Sarcoma
Some tumors regressed to the point that cancer cells could not be detected microscopically.
Team Successfully Converts Human Skin Cells into Embryonic Stem Cells
The breakthrough marks the first time human stem cells have been produced via nuclear transfer and follows several unsuccessful attempts by research groups worldwide.
Results of the ROTAVAC Rotavirus Vaccine Study in India
Statement of Anthony S. Fauci, M.D. Director, National Institute of Allergy and Infectious Diseases National Institutes of Health.
Flu in Pregnancy May Quadruple Child’s Risk for Bipolar Disorder
NIH-funded study adds to evidence of overlap with Schizophrenia.
1H NMR Spectroscopic Quantification of Plasma Metabolites in Dialysate during Hemodialysis
Researchers from Tohoku University have examined the feasibility of using TSP as a quantitative standard of dialysate.
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