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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Shubha Jain, F. Azam, Dr. H. N. Unni

We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. Numerical simulation has been performed to analyse the study of mixing performance of micro-fluidics channel.

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A combined cell placement and migration assay device for cancer cell anti-migration drug screening
Colin L. Hisey1,2, Oihane Mitxelena-Iribarren2,3, Miguel Martinez-Calderon2, Sergio Arana2,3, Maite Mujika2,3, S.M. Olaizola2, Derek J. Hansford1

We have developed a microfluidic device capable of hydrodynamically trapping cells over polymer lines which encourage 1D migration based on topographical cues. These lines have been fabricated with both femtosecond laser ablation and dewetting/stamping. This type of precise control will allow precise microfluidic assessment of potential drug targets to limit cancer cell migration.

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DNA-free CRISPR-Cas9 genome engineering in zebrafish
Amanda Haas, Alex J. Blasky*, Rytis Prekeris*, John A. Schiel, Melissa L. Kelley, and Anja van Brabant Smith | Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite 100, Lafayette, CO 80026, USA *University of Colorado - Anschutz Medical Campus, Department of Cell & Developmental Biology, Denver, CO, USA

Poster describing the advantages of a DNA-free gene editing system and the application of this system in zebrafish.

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Analysis of Terpenes Using Gas Chromatography with Vacuum Ultraviolet
Changling Qiu, Jonathan Smuts, Phillip Walsh, and Kevin A. Schug

The VUV absorption spectra for different terpenes were distinctive and differentiable. GC-VUV demonstrated the capabilities for qualitative and quantitative analysis of terpenes in turpertine mixtures. Chromatographic coeluting signals can be deconvolved by the VUV data analysis software.

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Mobility of Aeroallergens in Home: Effect of Location of Air Sampling and Implication for Evaluation of Patient Exposure
Julian Gordon1, Paul Detjen, Andrea Wachter & Prasanthi Gandhi1

The Inspirotec sampler permitted the easy testing of multiple locations within a household. Air sampling simultaneously at 12 locations by other technologies would have been technically challenging. These were run by an untrained operator.

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Lutjanus jocu (Dog snapper) as a bio-indicator of emerging contaminants and changes in environmental condition.
M. Sánchez-García1, S. J. Zottoli2 & L.Roberson-Murillo1

Our research assesses the use of Lutjanus jocu as a bioindicator of changes in environmental condition and water contaminated with toxic substances in estuarine ecosystems.

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Selective Debenzylation of N-Benzyloxypyrazinones in Flow
Anh Hung MAI - Cedrick VERYSER - Wim DE BORGGRAEVE

Selective and reproducible debenzylation of benzyloxypyrazinones by using catalytic transfer hydrogenation in flow chemistry to yield N-hydroxypyrazinones. The flow methodology enabled us to avoid overreduction of the compounds to pyrazin-2(1H)-ones.

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600 base reads on the Ion S5™ Next-Generation Sequencing System enables accurate HLA typing of 96 samples on one 530™ chip
Peter B. Vander Horn, Cisilya Duncan, Jamsheed Ghadiri, Amneet Gulati, Diana Jeon, April Jung, Mindy Landes, Tommie Lincecum, Geoffrey Lowman, Vadim Mozhayskiy, Linus Ong, Xinzhan Peng, Maryam Shenasa, Prasanna Thwar

We have demonstrated that by combining improvements in templating and sequencing biochemistry we are able to sequence templates longer than 600 bases with high accuracy on an Ion S5 530 chip.
These improvements open the S5 use space to include haplotyping applications that require longer reads. As a demonstration of that, we accurately typed 96 HLA samples on one 530 chip.

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Differences in the Neurophysiology of Infants with Down Syndrome May Predict Protective/Risk Markers for Subsequent Alzheimer’s Disease
Esha Massand1,2, Hana D’Souza1,2, George Ball1,2, Maria Eriksson1, Charlotte Dennison1, Joanna Ball1 & Annette Karmiloff-Smith1,2

Our work set out to understand early individual differences in memory abilities of infants with DS that may be predictive of subsequent cognitive phenotypes of AD.

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Scientific News
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Blood Pressure Drug May Boost Effectiveness of Lung Cancer Treatment
Researchers at Imperial College London have suggested that the blood pressure drug may make a type of lung cancer treatment more effective.
Insight into Eye Diseases
Scientists recreate zebrafish cell regeneration from retinal stem cells in mice.
New Discovery May Benefit Farmers Worldwide
Scientists have shown how a crop-microbe 'team' protect against fungal infection.
Antibodies Paving the Way to HIV Vaccine
Researchers uncover factors responsible for the formation of broadly neutralizing HIV antibodies in humans.
Designing Drugs with a Whole New Toolbox
Researchers develop methods to design small, targeted proteins with shapes not found in nature.
Protein Studies Discover Molecular Secrets
Two protein studies have mapped proteins that reveal the secrets to recycling carbon and healing cells.
Tapping Evolution to Improve Biotech Products
Researchers show how 'ancestral sequence reconstruction' can be used to guide engineering of a blood clotting protein.
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