|Improved Ligation Specificity with Chemically Modified Ligation Components|
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe
Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
|Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA|
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi
Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.
|A Dynamic Glucose-Sensitive Insulin Delivery System: poly(NIPAm/MAA) Nanoparticle Membrane|
It can be stated that there exists a spatial and temporal differential paradox of dynamic glucose levels in the body. Conventional insulin injections are unable to resolve this paradox.
|Novel Antimalarial Drug Candidates Generated In Silico by Analysis of Public HTS Data|
Robert Fraczkiewicz, Michael S. Lawless, Robert D. Clark, and Walter S. Woltosz
This research aims to provide proof-in-principle that in silico tools could be applied to public data so as to efficiently identify active chemistry with good ADMET properties.
| Finally, a User-Friendly Way of Computing and Presenting Individual Group Contributions to Polyprotic Ionization of Drugs|
Robert Fraczkiewicz, Marvin Waldman, Robert D. Clark
It is tempting to “assign” the macroscopic ionization constants (apparent pKa‘s obtained from titration experiments) of molecules to specific ionizable groups; however, this is strictly appropriate only in the case of monoprotic molecules.
|Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation|
Wu-Bo Li and Michael Goldblatt
Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.
|Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs|
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele
Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.
|Advanced Copy Number Variant Analysis with qbasePLUS 2|
Barbara D’haene, Jo Vandesompele and Jan Hellemans
Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.
|ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer|
Barbara D’haene, Pieter Mestdagh, Daniel Muth, Frank Westerman, Frank Speleman and Jo Vandesompele
This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.